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Biomedical subjects

D Huisingh

Publications and source records attributed to D Huisingh.

4 recordsLinked to original sources

Improved method for immunoelectrophoretic research.

A rapid, semiquantitative method for immunoelectrophoretic research is described. It combines the great resolving capacity of polyacrylamide gel disc electrophoresis with rapid immunoprecipitation in a thin agar film surrounding the acrylamide gel.

Journal Article↗

Murine toxicit of Agrobacterium tumefaciens.

Eleven strains of the crown gall organism, Agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. Five other species had some lethal strains. The lethal effect of A. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-killed cultures were lethal. The murine toxin disappeared when A. tumefaciens was grown at 36 C and reappeared when the organism was subsequently incubated below 30 C. The murine toxin itself was not inactivated by exposure to 100 C for 30 min. The toxin was associated with the cells and was not excreted into the medium. Centrifugal fractionation revealed that the toxin was associated with the smaller cells in 3-day stationary-phase cultures. These data suggested a possible relationship between toxin production and the production of the agents responsible for the initiation of plant tumors.

Animals↗