Biomedical subjects

D Huang

Publications and source records attributed to D Huang.

At least 127 records · Page 7Linked to original sources

PubMed1998

[The examination of EBV-DNase gene fragment in the paraffin embedded NPC, pre-cancerous and high risk population nasopharyngeal tissues].

OBJECTIVE: To examine if EB virus enters nasopharyngeal epithelial cells in the course of the tumorigenesis in nasopharyngeal carcinoma (NPC). METHODS: Based on the annual investigation of Si-Hui county, a cancer-prevention base of the Sun Yet-sen University of Medical Sciences and Tumour Hospital outpatient clinic, more than 450 paraffin-embedded tissues with NPC, and with pre-cancerous lesions in high risk populations were collected for the detection of EBV-DNase gene fragment by an optimized PCR method. RESULTS: In 145 of the 149(97.3%) cases with invasive NPC, and 2 of 4 cases with in situ NPC, EBV-DNase gene was detected. However, in Sihui, Canton, a high-risk county for NPC, only 2 of the 155 subjects with nasopharyngeal precancerous state was the gene positive, and none of the 47 cases examined in the out-patient department of the Tumor Hospital with precancerous lesions in the nasopharynx was positive. CONCLUSION: EBV is seldomly detected in the non-malignant and precancerous lesions in the nasopharynx in high-risk populations. It implies that EBV is probably not etiologically involved in the genesis of NPC.

Deoxyribonucleases↗

PubMed1998

[Rapid determination of active constituents in compound ibuprofen tablets by capillary zone electrophoresis].

A rapid capillary zone electrophoresis (CZE) method for the determination of ibuprofen and pseudoephedrine hydrochloride in compound ibuprofen tablets has been developed. CZE was performed in 0.025 mol/L phosphate buffer solution (pH 8.1). The two compounds were completely separated within 3 minutes, and quantified with a UV-detector at 210 nm by means of external standard method. Relative standard deviations of 2.9% for pseudoephedrine hydrochloride and 1.9% for ibuprofen were achieved for 11 determinations of the sample solution containing 9.5 mg/L pseudoephedrine hydrochloride and 66.7 mg/L ibuprofen, and recoveries of 103.1% and 97.6% were obtained respectively for the spiked pseudoephedrine hydrochloride and ibuprofen. The proposed method has been applied for the analysis of compound ibuprofen tablets, and the analytical results are in good agreement with those obtained by HPLC method.

Drug Compounding↗

PubMed1998

[Clinical study of jiuxin fumai injection in treatment of cardiogenic shock].

OBJECTIVE: To increase the cure and improvement rates and to decrease the mortality rate in the treatment of cardiogenic shock. METHODS: Thirty-one patients with cardiogenic shock were studied, and received Jiuxin Fumai injection (JXFM) in research group (n = 20) or dopamine (DA) in control group (n = 11). RESULTS: Eighteen effective cases (90.0%) and 2 ineffective cases (10.0%) in JXFM group, 9 effective cases (81.8%) and 2 ineffective cases (18.2%) in DA group. There was significant difference statistically, P > 0.05. CONCLUSIONS: JXFM and DA had the same effect in elevating blood pressure, enhancing pump function of the heart and ameliorating collapse syndrome. They are safe, effective, convenient for use and prompt in responding. However, JXFM showed raising blood pressure faster, higher as well as more stable, and the dual phase phenomenon in adjusting heart rate, which could slow down tachycardia as well as speed up bradycardia were observed.

Adolescent↗

PubMed1998

[Jugular bulb anomaly].

OBJECTIVE: To differentiate and treat correctly the jugular bulb anomalies, as well as to avoid the unnecessarily enlarged operations. METHODS: Four cases of jugular bulb anunalies were reported and analysed. RESULTS: Two of 4 cases were misdiognosed as jugular glomus tumors; One was considered to be jugular bulb anomaly, but was done with an enlarged operation; another one was still accompanied with benign intracranial hypertension syndrome, was inappropriately treated. CONCLUSION: The jugular bulb anomaly was often misdiagnosed, therefore the anomaly should be differentiated from the jugular glomus tumor carefully.

Adult↗

PubMed1998

[Chemical constituents of Rheum wittrochii Lundstr(II)].

OBJECTIVE: To study the chemical constituents in the root of Rheum wittrochii. METHOD: Chromatography and spectroscopic analysis to isolate and elucidate the chemical constituents in the plant. RESULT: Six compounds, namely 3, 5-dihydroxy-4'-methoxystilbene; 3, 5, 4'-tridroxystilbene; piceatannol; desoxyrhaponticin; piceatannol-4'-O-beta-D-glucopyranoside; 2, 5-dimethyl-7-hydroxychromone, have been isolated from ethyl ether and ethyl acetate fractions. CONCLUSION: These compounds were isolated from the title plant for the first time.

Drugs, Chinese Herbal↗

PubMed1998

[Chemical constituents of Rheum wittrochii Lundstr(I)].

OBJECTIVE: To study the chemical constituents in the root of Rheum wittrochii. METHOD: Chromatography and spectroscopic analysis were used to isolate and elucidate the chemical constituents in the plant. RESULT: Six compounds, namely chrysophanol, physcion, emodin, chrysophanol-8-O-beta-D-glucopyranoside, physcion-8-O-beta-D-glucopyranoside and beta-sitosterol, have been isolated from petroleum ether and ethyl acetate fractions. CONCLUSION: All the above-cited compounds were isolated from the title plant for the first time, and anthraquinone compounds are some of the major constituents of R. wittrochii.

Anthraquinones↗

PubMed1998

[Clinical analysis of Doppler waveform in arterial occlusive disease in the lower limbs].

OBJECTIVE: In order increase the diagnostic standard of Doppler ultrasound to arterial occlusive disease in the lower limbs. METHODS: 120 normal lower limbs and 66 lower limbs with arterial occlusive disease were measured by directional Doppler blood velocity detector in different segments. The peak forward velocity(PFV), peak reverse velocity(PRV), acceleration time(AT), and deceleration time(DT) were determined and compared. RESULTS: The blood flow velocity of the lower limb arteries was gradually slowed down from the proximal to the distal in the normal group. The ratio of distal to proximal PFA, PRV, AT and DT was less than 1.0. PFV and PRV in the disease group were slower than in the normal group(P < 0.01). AT and DT in the disease group were longer than in the normal group(P < 0.01). It was discovered that the ratio of distal to proximal DT was greater than 1.0 in arterial stenosis(> 50%) or occlusion (collateral artery development). CONCLUSION: The results were compared those of arteriography. The diagnostic coincidence ratio of Doppler ultrasound for localization was 91%(60/66).

Adult↗

PubMed1998

[Nasal neuroendocrine carcinoma].

OBJECTIVE: To recognize and understand further nasal neuroendocrine carcinoma (NNC), improve the diagnosis and therapeutic effect. METHODS: Eight cases of the nasal neuroendocrine carcinomas (7 males, 1 female) were analyzed. Among them, 6 cases had tumors in left side of nose, 2 in right side. The average course was only 2.8 months, but the lesions were often extent. RESULTS: The patients were pathologically confirmed to be neuroendocrine carcinomas. 5 cases were of small cell type, 2 undifferentiated, 1 poorly differentiated. One patient died 14 months after treatment. 2 cases were lost for follow-up. The others were still in treatment. CONCLUSION: The nasal neuroendocrine carcinoma has rapid progress and poor prognosis. Clinically, combined treatment should be adopted, the effect of simple operation was poor.

Adolescent↗

PubMed1998

[Stripping operation for auricular angioma].

OBJECTIVE: To improve the treatment of auricular angioma. METHOD: Twelve cases of auricular angioma were clinically analyzed and several treatments compared. RESULT: Eight of 12 cases were cured by the stripping operation, in which 7 cases had histories of recurrences with other methods. The lesions in 3 of 12 cases were controlled with the current treatments. The auricular in one case was totally excised due to the auricular fester, infection and necrosis of the cartilage. The cervico-facial trunk of facial nerve in one case during the stripping operation was surgically injured and sutured immediately by the end to end anastomosis. Facial paralysis disappeared half year after the operation. These cases were followed up from 8 months to 4 years. Only one case had local recurrence. CONCLUSION: Stripping operation can provide radical cure and is recommended for the treatment of auricular angioma.

Adolescent↗

PubMed1998

[Expressions of fibronectin and laminin in the middle ear mucosa with experimental middle ear effusions: immunohistochemical study].

OBJECTIVE: To understand the role of two components of extracellular matrix: fibronectin (FN) and laminin (LN) in the proliferated mucosa of otitis media with effusions. METHODS: A model of otitis media with effusions was established by coagulating the orifices of the eustachian tube under an endoscope in 30 guinea pigs. The expressions of FN and LN in the proliferated mucosa from these ears were detected using Streptavidin peroxidase (SP) method. RESULTS: In 60 ears with the orifices of the eustachinan tube coagulated, 28 ears showed otitis media with effusions. The increased expressions of FN on the fibroblasts and LN on the basement membranes and neovasculations were noted in the ears with otitis media with effusions. CONCLUSION: FN and LN may involve in the proliferated response of middle ear mucosa with otitis media with effusions. It may play a certain role in the occurrence of middle ear mucosa proliferations and adhesions.

Animals↗

PubMed1998

[Evaluation of the effect of causes of death of advanced schistosomiasis patients on the life span of population by using potential years of life lost analysis].

AIM: To evaluate the effect of the causes of death of advanced schistosomiasis patients on the life span of population in an area where schistosomiasis had been under control by applying the indicators of potential years of life lost (PYLL) and rate of PYLL (PYLLR). METHODS: Using the formula of calculation of PYLL and PYLLR, the causes of death of 487 patients of advanced schistosomiasis in 3 previously endemic townships of Shanghai suburb. RESULTS: Of 487 cases who died between 1955 and 1995, the PYLLR of all causes of death was 256.3@1000. The main causes of death were hepatic failure (100.3@1000), liver carcinoma (43.4@1000) and cancer of other organs (24.8@1000). In the 1960s, the PYLLR of advanced schistosomiasis complicated with hepatic failure and upper digestive tract hemorrhage was 222.2@1000 and 34.5@1000, the PYLLR of the complications of schistosomal liver cirrhosis, hepatic failure and hemorrhage accounted for 67.5% of the total PYLLR, being apparently higher in the non-splenectomy group than in the splenctomty group. After the schistosomiasis was under control, both PYLL and PYLLR decreased linearly. As compared with the 1960s, the PYLLR of all causes of death and the causes of death of the complications of schistosomal liver cirrhosis in the 1990s has declined by 62.9% and 83.2%, respectively, the PYLLR of upper digestive tract hemorrhage was found only 0.3@1000 in the last 5 years. CONCLUSION: PYLL and PYLLR analysis could be used to quantify the effect of complications of advanced schistosomiasis and other causes of death on the life span of population.

Adolescent↗

PubMed1998

[Effect of aqueous extract of Lepidium apetalum on dog's left ventricular function].

Left ventricular myocardial contractility, function of pump-blood and coronary flow in dog were increased by i.v. aqueous extract of Lepidium apetalum (AELA) 0.2 ml/kg (containing crude medicinal herb 2 g/ml). The effects were similar to i.v. isoperaline 10 micrograms/kg, but AELA did not obviously change heart rate, arterioveno-oxygen partial pressure difference and arteriovenooxygen solubility. The results indicated the AELA had advantages of cordial effect and increasing coronary flow instead of enhancing oxygen consumption of cardiac muscle.

Animals↗

PubMed1998

[Genetic polymorphism of STR loci D19S253 and D8S1179 for Han population in Wuhan].

OBJECTIVE: This article reports a population genetic study on two short tandem repeat loci, D19S253 and D8S1179, in a sample of unrelated Chinese individuals(n=225-233) living in Wuhan. METHODS: PCR and PAGE. RESULTS: Nine alleles were identified for the two loci, the ranges of fragment size were 209bp-241bp and 174bp-206bp respectively. The genotypes distributions of D19S253 and D8S1179 were in accordance with Hardy-Weinberg equilibrium. Family studies confirmed Mendelian inheritance of alleles. No mutation was detected in 100 meiosis for the two STR loci studied. The observed heterozygosities of D19S253 and D8S1179 were 0.8089 and 0.8712. The polymorphism information content(PIC) in Chinese was calculated as 0.7754 for D19S253 and 0.8258 for D8S1179. The power of discrimination(PD) and mean exclusion chance(MEC) were 0.9309 and 0.6133 for D19S253 and 0.9515 and 0.6874 for D8S1179 respectively. The combined PD and cumulative chance of exclusion were calculated as 0. 9966 and 0.8791. CONCLUSION: The results demonstrate that the combined use of the two polymorphic systems provides a high power of discrimination and power of exclusion for use in forensic identification and paternity testing.

Alleles↗

PubMed1997

Glucose-6-P control of glycogen synthase phosphorylation in yeast.

The SNF1 gene encodes a protein kinase necessary for expression of glucose-repressible genes and for the synthesis of the storage polysaccharide glycogen. From a genetic screen, we have found that mutation of the PFK2 gene, which encodes the beta-subunit of 6-phosphofructo-1-kinase, restores glycogen accumulation in snf1 cells. Loss of PFK2 causes elevated levels of metabolites such as glucose-6-P, hyperaccumulation of glycogen, and activation of glycogen synthase, whereas glucose-6-P is reduced in snf1 cells. Other mutations that increase glucose-6-P, deletion of PFK1, which codes for the alpha-subunit of 6-phosphofructo-1-kinase, or of PGI1, the phosphoglucoisomerase gene, had similar effects on glycogen metabolism as did pfk2 mutants. We propose that elevated glucose-6-P mediates the effects of these mutations on glycogen storage. Glycogen synthase kinase activity was reduced in extracts from pfk2 cells but was restored to that of wild type if the extract was gel-filtered to remove small molecules. Also, added glucose-6-P inhibited the glycogen synthase kinase activity in extracts from wild-type cells, half-maximally at approximately 2 mM. We suggest that glucose-6-P controls glycogen synthase activity by two separate mechanisms. First, glucose-6-P is a direct activator of glycogen synthase, and second, it controls the phosphorylation state of glycogen synthase by inhibiting a glycogen synthase kinase.

Enzyme Activation↗

PubMed1997

Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p.

The biosynthesis of glycogen involves multiple proteins that associate with each other and the glycogen macromolecule. In efforts to understand the nature of these proteins, a two-hybrid screen was undertaken to detect proteins able to interact with Gsy2p, a major form of glycogen synthase in Saccharomyces cerevisiae. Two positives expressed proteins derived from genes designated PIG1 and PIG2, on chromosomes XIIR and IXL respectively. PIG1 codes for a protein with 38% identity over a 230 residue segment to Gac1p, a protein thought to be a type 1 protein phosphatase targeting subunit whose loss impairs glycogen synthesis. Pig2p has 30% identify to the protein corresponding to an open reading frame, YER054, on chromosome V. Deletion of PIG1 on its own had little effect on glycogen storage but, in combination with loss of GAC1, caused a more severe glycogen-deficient phenotype than seen in gac1 mutants. This result is consistent with Pig1p being functionally related to Gac1p and we propose that Pig1p may be a type 1 phosphatase regulatory subunit. Delection of PIG2, YER054, or both genes together caused no detectable change in glycogen metabolism under the conditions tested. Gac1p, Pig1p, Pig2p and the YER054p are the only four proteins coded by the yeast genome that share a conserved segment of approximately 25 residues, designated the GVNK motif, that is identifiable also in RGI, the mammalian type 1 phosphatase targeting subunit.

Amino Acid Sequence↗

PubMed1997

Major role of collagen IIB in the elevation of total type II procollagen messenger RNA in the hypertrophic phase of experimental osteoarthritis.

OBJECTIVE: To define the relative contributions of procollagen IIA and IIB messenger RNA (mRNA) in the 8-fold elevation of total (IIA + IIB) type II procollagen mRNA in cartilage from joints with early experimental osteoarthritis (OA). METHODS: Unilateral cruciate ligament transection was performed on skeletally mature animals. Total RNA was extracted from articular cartilage from control joints and from joints with experimental OA. Northern blot hybridization with oligoprobes was used to distinguish between types IIA and IIB mRNA. RESULTS: Levels of IIA mRNA were similar in control and experimental cartilage. Type IIB mRNA accounted for the increase in total type II mRNA expression in OA joints. CONCLUSION: Although OA chondrocytes adapt to making immature forms of aggrecan proteoglycan, they do not revert to the prechondrocyte stage characterized by IIA mRNA expression.

Animals↗

PubMed1997

Structure-binding relation of philanthotoxins from nicotinic acetylcholine receptor binding assay.

Philanthotoxins are noncompetitive inhibitors of the nicotinic acetylcholine receptor and the various glutamate receptors. Analogues carrying photoaffinity labels, fluorine atoms for solid-state NMR studies of ligand/receptor interaction, and large head groups such as porphyrins and planar bulky aromatic rings (BIG analogues) for clarifying mode of entry and orientation of analogues in receptors have been synthesized, assayed against the nicotinic acetylcholine receptor, and brief comments are given for the assay results.

Amino Acids↗

PubMed1997

Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma.

Human immunodeficiency virus type 1 (HIV-1) pol mutations are responsible for HIV-1 resistance to current antiretroviral drugs. HIV-1 RNA extraction with QIAamp HCV kit spin columns (Qiagen, Chatsworth, Calif.) followed by reverse transcription-PCR successfully recovered a 1,008-bp pol fragment from the plasma of 31 of 34 HIV-1-infected patients that was suitable for sequencing and recombinant-virus studies. The minimum HIV-1 RNA concentration required for gene recovery was 30 to 40 copies/ml, which was similar to the minimal HIV-1 RNA concentration required when phenol-chloroform or silica beads are used for RNA extraction.

Chloroform↗