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Biomedical subjects

D Heidemann

Publications and source records attributed to D Heidemann.

35 records · Page 2Linked to original sources

[Human gingiva in cell culture].

Human gingival cells, particularly epithelial cells of the gingiva, have rarely been cultured successfully. Indispensible are single-layered cell sheets which allow to observe certain cells of a cell layer over a longer period of time when testing drugs and materials. One possibility was described for the cultivatability of these highly specialized cells, the long-term culturing of which has seldom been successful. Intracellular storage of glycogen in the cultured cells was demonstrated to establish the metabolic activity firms the specificity of the cells.

Cells, Cultured

[Composite filling materials in cell cultures].

Using cell cultures of human gingiva, the composites Nuva Fil, Vytol, and Estic microfil were studied in terms of tissue tolerance. While the cytotoxicity for nonpolymerized and polymerized Nuva Fil and Vytol was extremely low, multinuclear fibroblasts were observed with Estic microfil.

Cell Nucleus

[Cell culture of human gingival epithelium].

Gingival explants were taken from 8 volunteers in order to find a method to grow gingival epithelial cells. We succeeded in what we regarded as optimal conditions in achieving a monolayer cell thickness in the culture by means of BHK medium and foetal calf serum.

Cells, Cultured

[Effect of local hemostyptics on human gingiva fibroblasts in culture].

Local hemostyptics and materials for filling surgical defects are frequently used by dentists after extractions and in the treatment of cystic cavities. Three of these materials, Kollagen Vlies, Tabotamp Gaze, and Gelita Tampon were studied in cultures of human gingiva fibroblasts for their biological tolerance.

Cells, Cultured

[Comparison of the biological tolerance of titanium and titanium alloys in human gingiva cell cultures].

Mirror-finished solid specimens of pure titanium and the titanium alloys Ti-6Al-4V as well as Ti-5Al-2.5Fe showed no effects on the growth behavior and cell morphology of human gingival epithelial cell and fibroblast cultures. The growth of the cells contacting all three materials was uninhibited. SEM revealed growth of fibroblasts on the surfaces of the specimens, too. No differences could be found between the biocompatibility of titanium alloys and that of pure titanium. The formation of a stable surface oxide layer providing resistance to corrosion may be decisive.

Alloys