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Biomedical subjects

D Harris

Publications and source records attributed to D Harris.

At least 307 records · Page 17Linked to original sources
PubMed1977

Immunologic tolerance to heterologous immunoglobulin: its relation to in vitro filtration by macrophages.

The cellular and molecular basis for the difference in ability of BCG to induce tolerance in BALB/c and DBA/2 mice has been examined by in vitro biofiltration. It was found that incubation with the adherent cells from BALB/c but not DBA/2 spleens could remove the material from BGG which inhibited tolerance induction in BALB/c mice. This material was shown to represent only a trace component in BGG, was present in only certain commercial batches of BGG, and was apparently unrelated to the presence of aggregates or endotoxin.

Animals↗
PubMed1976

Effect of ionizing radiation on liver microcirculation and oxygenation.

Platelet aggregation and adhesiveness, as well as TPO2 responses to hypoxia were measured as microcirculation parameters in beagle dogs subject to Co60 ionizing radiation to a dose of 4600 rads in 5 weeks. Simultaneously, changes in blood chemistry and coagulation were also determined. Marked changes in all studied parameters in the post radiation period lead to the conclusion that radiation liver damage is at least in part mediated through microcirculation disturbances.

Animals↗
PubMed1975

Prenatal diagnosis of cystinosis.

Cystinosis was diagnosed in a small quantity of cultured amniotic cells from a 22-week-old fetus by a modified pulse-labeling technique in which intracellular 35SL-cystine retention was measured. As a result of the above finding, the pregnancy was terminated by administration of prostaglandin. The diagnosis was confirmed when the nonprotein-free cystine content of the kidney, liver, placenta, spleen, thymus, and gut, as well as that of a large amount of cultured amniotic cells, was found to be 100-fold higher than normal levels.

Abortion, Induced↗
PubMed1975

Evaluation of review courses for the ECFMG examination.

In this paper three review courses for the Educational Council for Foreign Medical Graduates (ECFMG) examination are described. A comparison of the pass rates on the January 1973 ECFMG examination for examinees taking the courses with those not taking the courses shows a statistically significant difference (47 percent passing for those taking the courses in contrast to 18 percent passing for those not taking the courses). Although the courses appear to be effective, the limitations of the ECFMG examination as a screening device for foreign medical graduates (FMGs) make it unwise to expand the number of such courses. The cramming of basic medical knowledge into students is not a useful solution to the problem of physician shortages. The focus of attention on the FMG in the health care system must shift from a search for expedient ways of providing for greater utilization of FMGs to the deleterious effects of this utilization on the quality of patient care.

Achievement↗
PubMed1974

Uptake and utilization of exogenous cystine by cystinotic and normal fibroblasts.

The uptake of l-[(35)S]cystine was studied in six cystinotic and six normal fibroblast lines grown for five days either on cover slips or in 32-oz plastic flasks. Cystinotics showed greater uptake than normals. The apparent K(t) for cystine entry in both types of cells was 0.043 mM but cystinotic cells showed a higher maximum velocity of entry. A comparison of the fate of l-[(35)S]cystine incubated for 20 min with monolayers of cells showed 30% and 15% of the intracellular (35)S to be l-cystine in cystinotic and normal cells, respectively. The (35)S effluxed more slowly from cystinotic than from normal cells after a 20-min preloading with l-[(35)S]cystine. Identification of (35)S compounds in efflux media after 3 min showed 75% of the total (35)S was l-cystine with the remainder in cysteine and acidic sulfur metabolites of cystine with no essential difference between cystinotics and normals. In paired experiments, the specific activity of the effluxed l-[(35)S]cystine after both efflux periods was the same as that entering the cell, thus indicating that the free l-[(35)S]cystine had not exchanged with the pre-existing pool in the cystinotic cells. During 3 min efflux, the l-cystine pool in normal cells was depleted mainly by loss of free cystine. In cystinotic cells, a new steady state was attained after 21 min of efflux and the intracellular l-[(35)S]cystine had the same percentage of total radioactivity seen after the initial 20-min uptake. After the rapid efflux of l-[(35)S]cystine from normals, [(35)S]cysteine and other labeled cystine metabolites appeared in the efflux media. By the end of a 3-min efflux, cystinotic cells had incorporated more label into reduced glutathione than had normal cells. However, when the new steady state was attained in cystinotics, the amounts of (35)S in glutathione were not markedly different in the two types of cells. Approximately 95% of the total label could be accounted for in free sulfur compounds. The data show an increased uptake and decreased efflux of cystine from cystinotic cells. However, it is not possible to conclude if these differences are due to primary changes in membrane function or to the reflection of metabolic defects without further investigation.

Adolescent↗
PubMed1972

Colloid osmotic pressure in erythroblastosis fetalis.

Measurements were made of total proteins, albumin, and colloid osmotic pressure on cord blood samples from 15 infants with erythroblastosis fetalis (six of whom were hydropic) and from 151 non-rhesus non-hydropic control infants. The erythroblastotic infants had levels of total protein and albumin which fell within the normal range for gestational age, but their colloid osmotic pressures were abnormally low. It seems that low colloid osmotic pressure may provide a reasonable explanation for the occurrence of hydrops fetalis.

Birth Weight↗