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Biomedical subjects

D H Moore

Publications and source records attributed to D H Moore.

At least 253 records · Page 14Linked to original sources
PubMed1965

Identity of the filterable hemolytic anemia agent of Sacks with Haemobartonella muris.

Moore, D. H. (The Rockefeller University, New York, N. Y.), R. N. Arison, H. Tanaka, W. T. Hall, and M. Chanowitz. Identity of the filterable hemolytic anemia agent of Sacks with Haemobartonella muris. J. Bacteriol. 90:1669-1674. 1965.-In 1960 a new hemolytic agent in rats was reported. It was thought to be a filterable, nonsedimentable, replicating infectious agent, different from Haemobartonella muris. Rats which recovered from the infection developed a resistance to several kinds of transplantable tumors. It is here shown that this agent has the same properties as those reported in the literature and reconfirmed by us for H. muris. The size of the agent is approximately 500 mmu as determined by correlating bioactivity with Gradocol membrane filtrates and fractions from a diffusion cell, and its density is about midway between that of whole serum and distilled water (approximately 1.020) as determined by the sedimentation of bioactivity in an ultracentrifuge. Diffusion at 30 C indicated a lack of motility by the agent bodies. Rats were found to be protected against infection with the agent by daily administration of chlortetracycline and by prior infection with H. muris. The agent bodies were indistinguishable from H. muris in both the light and the electron microscope.

Anemia, Hemolytic↗
PubMed1965

Fine structure of Haemobartonella muris as compared with Eperythrozoon coccoides and Mycoplasma pulmonis.

Tanaka, H. (The Rockefeller University, New York, N.Y.), W. T. Hall, J. B. Sheffield, and D. H. Moore. Fine structure of Haemobartonella muris as compared with Eperythrozoon coccoides and Mycoplasma pulmonis. J. Bacteriol. 90:1735-1749. 1965.-Thin sections of filterable hemolytic anemia agent of rat, now identified as Haemobartonella muris, revealed (i) that the agent is spherical or ellipsoidal and 350 to 700 mmu in size, (ii) that it has a single limiting membrane enclosing granules and some filaments (neither cell wall nor nucleoid was found), and (iii) that it is found preferentially at the surface and sometimes within the cytoplasmic vacuoles of erythrocytes in the circulating blood and bone marrow, and multiplies there through binary fission. No specific structure suggestive of motility was found. From the morphological point of view, Eperythrozoon coccoides was found to be very similar to H. muris, whereas Mycoplasma pulmonis, also covered with a single limiting membrane, differs in its pleomorphism and multiplication. These and some other microorganisms taxonomically between bacteria and viruses were compared morphologically, and their possible classification is discussed.

Animals↗
PubMedDate not supplied

Volumetric flow in the umbilical artery: normative data.

OBJECTIVE: Provide normative data for the volumetric blood flow (cc/min and cc/min/kg) in the umbilical artery. METHODS: Flow was determined from an umbilical artery in 252 normal obstetrical patients from 18-40 weeks' gestation utilizing pulsed Doppler and color flow Doppler with an angle of insonation of 30-60 degrees. Simultaneous velocimetry studies (S/D ratio, resistance and pulsatility indices), fetal biometry, and an anatomic survey were obtained to further define the normal population. RESULTS: There was a steady increase in the flow (cc/min) in the umbilical artery as pregnancy progressed. Flow/kg showed a steady decline as fetal weight increased. Umbilical artery diameter increased until reaching a plateau at 32-34 weeks. Velocimetric results were consistent with known data. CONCLUSIONS: Volumetric blood flow in the umbilical artery can be determined with relative ease and normative data from 18-40 weeks is presented for the first time.

Adult↗
PubMedDate not supplied

Comparison of cellular and whole-animal bioassays for estimation of radiation effects in the polychaete worm Neanthes arenaceodentata (Polychaeta).

The polychaete worm Neanthes arenaceodentata was used in experiments to determine possible relationships between short-term genotoxicity tests and reproductive and lethal consequences of exposure to ionizing radiation. Groups of juvenile N. arenaceodentata received one of four different radiation doses (2, 4, 8, and 16 Gy) to determine dose-effect estimates for chromosomal aberration induction, and groups of both adult and juveniles received one of seven different radiation doses (1, 4, 8.4, 46, 102, 500, and 1000 Gy) to determine dose-effect estimates for reproduction, mortality, and life span. Effects on reproduction and genetic material were observed at the lowest doses and in the same range; detrimental reproductive effects were observed at 1 to 4 Gy, and the frequency of chromosomal aberrations was significantly increased at 2 Gy. Only high doses resulted in acute mortality (greater than 500 Gy) and decreased life span (greater than 100 Gy). Dose-effect estimates for chromosomal aberration induction were dependent on radiation dose and on the stage of the cell cycle at the time of irradiation. Dose-effect estimates for reproduction were dependent on dose and the potential for repopulation of gonadal tissue. It is concluded that short-term genotoxicity test can be predictive of detrimental reproductive effects in those model systems for which basic cell kinetics and reproductive parameters are well known.

Animals↗