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D Greiff

Publications and source records attributed to D Greiff.

27 records · Page 2Linked to original sources
PubMed1966

Neuraminidase activity in Diplococcus pneumoniae.

Kelly, R. T. (Marquette University School of Medicine, Milwaukee, Wis.), D. Greiff, and S. Farmer. Neuraminidase activity in Diplococcus pneumoniae. J. Bacteriol. 91:601-603. 1966.-A method for the quantitation of neuraminidase in the presence of N-acetylneuraminic acid aldolase is described. The neuraminidase content of Diplococcus pneumoniae was found to be dependent on the media employed for growth; the highest enzyme activity per milligram of bacterial protein was obtained with Todd-Hewitt broth. Neuraminidase production was stimulated in D. pneumoniae by the addition of N-acetylneuraminlactose, N-acetylneuraminic acid, or N-acetylmannosamine to the growth medium. Three rough strains of D. pneumoniae, which were nonpathogenic for mice, lacked neuraminidase activity. Seven of 12 smooth strains contained neuraminidase; enzyme activity was not detected in the remaining 5 smooth strains. There was no correlation between the presence of neuraminidase activity and the capsular type or between neuraminidase production and animal virulence.

Amines↗
PubMedDate not supplied

Factors affecting the statistical parameters and patterns of distribution of residual moistures in arrays of samples following lyophilization.

Numerous studies were undertaken to examine in depth the statistical parameters (mean, standard deviation, standard error of the mean, etc.) and the distributions of residual moistures of 3 mL samples of 2% albumin arranged in a 22 x 10 grid of samples (the array) and dried by sublimation of ice in vacuo. Because of the relatively large numbers of selected samples used for the determination of the patterns of distribution of residual moistures, it was necessary to develop a modified gravimetric method for measuring residual moistures. In the majority of circumstances, with the vials containing the samples positioned on a base defined by cartesian coordinates (x, y) and the contents of residual moistures measured on an axis perpendicular to the base (z), the distributions of contents of residual waters were described best by the equation for a 2-nd order polynomial. It was found that the contents of residual moistures of individual vials were "position oriented" and not "vial associated." It was also found that the statistical parameters and patterns of distributions of residual moistures were dependent on: (1) the orientation of openings in the split stoppers used for freeze-drying, (2) the shelf of the freeze-drying apparatus (top, middle, bottom) upon which the vials were placed, and (3) alterations in elapsed times and shelf temperatures.

Animals↗