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Biomedical subjects

D Glinoer

Publications and source records attributed to D Glinoer.

104 records · Page 6Linked to original sources
PubMed1976

Thyroxine-binding globulin biosynthesis in isolated monkey hepatocytes.

Thyroxine-binding globulin biosynthesis was demonstrated in hepatocytes isolated from normal adult Rhesus monkeys. Dispersed cells were obtained by in situ liver perfusion with collagenase, hyaluronidase and EDTA. Conditions for optimum cell survival and incorporation of radioactive leucine into newly synthesized proteins were defined. Protein synthesis, and specifically thyroxine-binding globulin synthesis, were shown to continue throughout the incubation period, while cell survival remained high (75% excluded trypan blue after 6h). Incubation medium, cytosol and a particulate fraction (extracted with digitonin) were analyzed for thyroxine-binding globulin. After extensive dialysis and purification by affinity chromatography, newly synthesized thyroxine-binding globulin was identified by specific double-antibody immunoprecipitation and by immunodiffusion and immunoelectrophoresis with autoradiography. Newly synthesized thyroxine-binding globulin was present after 4 h of incubation. After 6 h, the total synthesized had increased to 150% of the 4 h value, while the fraction present in the medium and increased to 300%, indicating probable thyroxine-binding globulin secretion

Animals↗
PubMed1976

Paradoxical modulation of thyrotrope responsiveness to TRH during the treatment of thyrotoxic patients: apparent absence of feed-back regulation.

Eight patients with active thyrotoxicosis have been followed up to one year after the onset of antithyroid treatment. At different time intervals during the investigation period, TRH tests were performed and total T4 and T3 basal levels were measured. The TRH-induced TSH release was delayed in all patients in spite of a normalization of the circulating levels of thyroid hormones. The delay varied according to the patients and lasted, in some cases, for as long as 24 weeks after the normalization of the thyroid hormone levels. Administration of thyroid hormones, together with methimazole, did not seem to prevent the pituitary thyrotropes responsiveness; moreover, it did not provoke an inhibition of the TRH-induced TSH release once the thyrotropes reactivity was fully restored. The feed-back mechanism does not seem to be the main modulator of the pituitary responsiveness to TRH in these circumstances.

Adolescent↗
PubMed1975

Adrenocortical and somatotrophic secretions in acute and chronic respiratory insufficiency.

Adrenocortical function and plasma growth hormone pattern were investigated in 15 patients with chronic obstructive lung disease, in a period of acute respiratory failure and again after recovery. During the acute period, secretion rate and plasma concentrations of cortisol were markedly enhanced; urinary excretion of cortisol metabolites was only slightly increased, suggesting an alteration of the catabolism of cortisol under these conditions; adrenocortical sensitivity to corticotropin and capacity of maximal adrenal secretion were normal. The increase of cortisol secretion was probably due to hypoxemia and/or hypercapnia acting through the hypothalamo-pituitary axis. During the chronic phase of respiratory insufficiency, adrenocortical secretion and responsiveness were within the normal range. Finally, respiratory failure did not stimulate the secretion of growth hormone.

17-Ketosteroids↗
PubMed1975

Ultrastructural aspects of the thyroid in a case of human congenital goitre with cretinism.

Transmission electron microscope and scanning electron microscope studies in a case of congenital goitre associated with cretinism revealed the existence of two cell types: the first with rounded endoplasmic cisternae and normal lysosomes; the second characterized by an abundance of lysosomes and/or lipofuscin granules. Intermediary aspects suggested that the second cell type was the progeny of the first type. As colloid droplets and apical pseudopods engulfing colloid were nearly absent, and as NBE127I was elevated in the plasma, it is suggested that iodoprotein secretion occurred by a process different from colloid phagocytosis as observed after acute TSH stimulation.

Congenital Hypothyroidism↗
PubMedDate not supplied

Alterations in circulating thyroid hormones and thyroxine-binding globulin levels during diabetic ketoacidosis.

During diabetic ketoacidosis, in 17 adult patients, significant decreases in serum TBG and total T4 levels were observed, without significant alteration of the T4 to TBG binding property. In addition, serum free T4 (FT4) was moderately elevated and the TSH response to TRH was markedly blunted. No correlation, however, was found between TSH blunting and FT4 elevation. Correlation of these serum anomalies required at least 5 days of adequate control of the diabetes. Thus, diabetic ketoacidosis in euthyroid patients is characterized by multiple alterations in thyroid function parameters and caution is recommended in the interpretation of thyroid tests during and in the days following this severe metabolic disorder.

Adult↗
PubMedDate not supplied

The management of hyperthyroidism due to Graves' disease in the former USSR in 1991: results of a survey.

A survey of the current management of Graves' disease was performed in the USSR among members of All Union Endocrine Society. The questionnaire was based on the format used previously for a survey of members of European Thyroid Association. The aim of a similar survey in the USSR was to obtain a comprehensive pattern of management of Graves' disease in Soviet endocrinology clinics and to compare medical attitudes in the former USSR to those in other European countries. One hundred and twenty questionnaires were mailed with 55 returned (46%). The responses originated from 33 cities, representing major endocrinology centers of the former Soviet Republics. Initial diagnosis was conducted both in hospitals (55%) and ambulatory care settings (45%). Thyroid scintigraphy was requested by 42.3% of the respondents; a majority of them (90%) used 131I. Thyroid ultrasonography was performed in more than 50% of cases. Measurements of cholesterol, total T4 and T3 were the most frequent laboratory tests requested to confirm the diagnosis. For the treatment with ATD, methimazole was the exclusive choice (PTU is not currently in use in the USSR). Beta-blocking agents were prescribed by a majority of respondents. For the long term treatment, a combination procedure of MMI and thyroid hormones was clearly preferred by almost 3/4 of the respondents. A fixed period of treatment was preferred by 62% of the respondents, with a duration of therapy of 18-24 months. Surgery for treatment of the index patient was chosen by only 6%, and radioiodine by 3%. The number of responses was too limited to attempt any characterization of the two latter modalities.

Adrenergic beta-Antagonists↗
PubMedDate not supplied

[Secondary effects of lithium on thyroid function. Mechanisms, diagnostic and treatment (author's transl)].

The authors reviewed the literature concerning the side effects of lithium on thyroid function. Two kinds of side effects are reported: the first ones are minor, temporary, without important modification of thyroid function and must not be treated; the second ones are major and followed by hypothyroidism. This situation is mostly in relation with the preexistence of an autoimmune thyroiditis or another thyroid abnormality. The authors describe the mechanisms responsible for lithium side effects, suggest different thyroid examinations that should be done in patients before and during lithium therapy and discuss the indications of thyroxine administration.

Humans↗
PubMedDate not supplied

[Hypothyroïdism during lithium treatment (author's transl)].

Five patients, who were treated with lithium at the time their hypothyroidism was discovered, are presented. Three of them had high levels of circulating antithyroid antibodies; one had been thyroidectomized several years ago and the last subject had congenital agenesis of one thyroid lobe. The relationship between hypothyroidism and preexisting thyroid abnormalities in patients receiving lithium is suggested.

Autoantibodies↗