Biomedical subjects

D Glick

Publications and source records attributed to D Glick.

At least 55 records · Page 3Linked to original sources

PubMed1977

Microchemical analytical techniques of potential clinical interest.

Microchemical techniques with the capability of single-cell analysis will become increasingly important in clinical laboratory practice. One of the current moves underway in this direction is determination of enzyme activities of cells by microscale spectrophotometry and spectrophotofluorometry for prenatal diagnosis of inherited enzyme deficiencies. Three less-common microanalytical techniques are considered here that are of potential interest in laboratory medicine. The first is dilatometry, which provides a means for enzyme or substrate assay involving measurement of the change in volume or density accompanying chemical reaction in solution. In this connection, the measurement of specific gravity itself to obtain certain chemical information is also of interest. The second of these techniques is the use of spectrophotometry to measure oxygen uptake for functional assays of cells. The third is the use of luminometry in a general system of analysis for determination of many important biochemical substances and activities. Each of these techniques can be used for microscale analysis without sacrifice of precision or accuracy; each is relatively simple, instrumentally ant technically, and could be automated.

Animals↗

PubMed1976

Patient motivation and response to surgical correction of prognathism.

Twenty-five patients operated upon between 1969 and 1973 for correction of mandibular prognathism were recalled in 1975 and asked to evaluate their attitudes toward the procedure and the postoperative results. All but one were satisfied with the results. Postoperative numbness of the lip and chin lasting more than 6 months was commonly noted following the intraoral sagittal split method. This did not influence the patient's satisfaction with the operation. The minimal postoperative scar following the extraoral oblique osteotomy was not a negative factor. Most patients underwent the surgical procedure for esthetic reasons. A large number (68 per cent) noted a change in personality postoperatively. A rationale for selective preoperative psychologic counseling is suggested.

Consumer Behavior↗

PubMed1976

Relation between idiopathic glossodynia and salivary flow rate and content.

From a series of 50 patients complaining of sore tongue, 13 were found to be suffering from idiopathic glossodynia. All were women in the postmenopausal stage. The salivary flow rate, protein, phosphate, and electrolyte content (Na, K, Ca, Mg) were measured in unstimulated saliva of these patients. Protein, potassium and phosphate concentrations were significantly higher than in the control group. The results indicate that hormonal disbalance might be a factor in the etiology of this type of sore tongue.

Aged↗

PubMed1975

Quantitative histochemical study of a vitamin E analog on rat gastric cyclic AMP.

The vitamin E analog, 2,2,7,8-tetramethyl-5,6-orthoquinonechroman, unlike vitamin E itself, had been reported to be a potent inhibitor of beef liver cyclic AMP-PDE in vitro (about 3.5 times more inhibitory than theophylline under the same conditions). In this in vivo study, single intraperitoneal injections were given to fasted rats, 15 min before killing, of a solution of 20, and of a suspension of 130, mg analog/kg in 4% ethanol. No significant increases over the 4% ethanol control values in cyclic AMP levels were produced in any of the histological regions of the glandular stomach. However, injection of the 4% ethanol itself produced a rise of about 50% (peak value 4.1 pmoles/mg wet wt, 0.16 pmoles/mug protein-nitrogen) in the cyclic AMP concentrations in the parietal cell region and had no significant effect in any of the other histological zones where the concentrations were much lower.

Animals↗

PubMed1975

Potential for clinical use of the analytical laser microprobe for element measurement.

Use of the laser microprobe for rapid emission spectroscopic analysis of elements in microscopic samples of biological material is described. The technique depends on vaporization of the microsample with a focused laser beam at a temperature that renders the vapor incandescent for spectrochemical analysis. Spectral line intensities are recorded photographically with densitometry of the negatives or photoelectrically. Current capability permits analysis of about 10(-8) to 10(-10) g of tissue, with detection limits of 10-12 to 10-15 g of element. Groups of elements can be simultaneously analyzed. Minimum sample preparation is required, and the analysis is done on the stage of a light microscope, usually on an air-dried sample on a plastic slide. We exemplify the technique in analysis of gold in cultured fibroblasts treated with gold salts and in human skin after treatment with gold salts for rheumatoid arthritis, in element changes in biopsies of transplanted human hearts, and in unique profiles of groups of elements in human cancer tissue.

Adenocarcinoma↗

PubMed1975

Effects of drugs influencing gastric secretion on the quantitative histological distribution of cyclic adenosine 3':5'-monophosphate in the rat stomach.

To help clarify conflicting evidence on the cyclic adenosine 3':5'-monophosphate (cyclic AMP) role in acid secretion in the rat stomach, and to establish the quantitative effects on cyclic AMP concentrations in histological regions of the glandular stomach of the 24-hr fasted rat of injections of drugs that stimulate or inhibit gastric secretion, the concentrations were measured by the firefly luminescence method using fresh-frozen microtome tissue sections cut serially through the stomach wall. The data are compatible with cyclic AMP involvement in the acid secretion. Parietal-mucous neck cell cyclic AMP levels (3.3,0.11 means, expressed as picomoles per mg of wet weight and picomoles per mug of protein-nitrogen, respectively) were the highest in any region. Pentagastrin or urecholine elevated these values slightly, and theophylline produced greater increases in all regions (parietal-mucous neck cell, 5.4, 0.22), but theophylline administered either with pentagastrin or urecholine further elevated the values (8.3, 0.31). Urecholine or pentagastrin plus theophylline each generated two approximately equal cyclic AMP concentration peaks (about one-half parietalmucous neck cell concentrations (2.7, 0.22), perhaps by negative feedback, but low dose (2 mg per kg) elevated these values more than pentagastrin or urecholine, and with theophylline they were greatly increased (9.1, 0.32). The inhibitors, atropine and prostaglandin E2, alone or with theophylline, had little influence on parietal-mucous neck cell cyclic AMP levels but caused an increase in chief cell and muscle zones.

Animals↗

PubMed1974