Biomedical subjects

D Gladman

Publications and source records attributed to D Gladman.

42 records · Page 3Linked to original sources

PubMed1983

Lymphocytotoxic and phagocytotoxic activity in progressive systemic sclerosis.

Sera of 66 patients with progressive systemic sclerosis (PSS) were tested for cold and warm reacting lymphocytotoxins (LCT). Cold LCT were found in 30 (45%) patients, 18 of whom also had warm LCT. Warm LCT alone were found in 14 patients. Twenty-nine sera with cold LCT were tested and reacted with both peripheral B and T lymphocytes. There was predominant killing of B cells in 52% and of T cells in 14%. Ten cold LCT were absorbed to and eluted from peripheral blood lymphocytes. All eluates were cytotoxic to B and T cells; 1 killed predominantly T cells and 2 killed predominantly B cells. Clinical-laboratory and HLA correlations with cold LCT showed no significant differences between the LCT-positive group and the LCT-negative group. Granulocytotoxins were rare in PSS, but warm reacting monocytotoxins were found in 33 cases (57%). Crossreactivity of cytotoxins was tested using eluates from various cells. The majority of eluates from lymphocytes were cytotoxic against polymorphonuclears (PMN) and monocytes. Some eluates from PMN and from monocytes had lymphocytotoxic activity. This suggests existence of common antigenic determinants on various cells against which cytotoxins are directed.

Antibodies↗

PubMed1982

Aberrations in lymphocyte subpopulations and function during psychological stress.

Eight trainees in psychiatry taking their final oral fellowship examinations were compared with 16 controls to determine the effect of stress on their immune system. Two measures of stress were utilized to distinguish the highly stressed subjects from those minimally stressed. T cell subpopulations, B cell numbers, mitogen reactivity, natural killer cell activity, plaque forming cell responsiveness, antigen specific T suppressor cell activity, and hormone levels were studied 2 weeks before and 2 weeks after the exam. The results demonstrated transiently elevated numbers of T and B lymphocytes but impaired plaque forming cell and mitogen responsiveness in the highly stressed group prior to their exam which normalized later. The results support the concept that stress may significantly alter the immune response in man.

Adult↗

PubMed1980

Impaired antigen-specific suppressor cell activity in patients with systemic lupus erythematosus.

Antigen-specific suppressor cell activity of peripheral blood mononuclear cells was investigated in twenty-nine patients with systemic lupus erythematosus (SLE) and sixteen normal, age- and sex-matched healthy controls. Suppressor cell activity was generated by priming peripheral blood mononuclear cells with high dose antigen (ovalbumin) and adding the washed primed or control (unprimed) cells to autologous optimally stimulated target plaque-forming cell (PFC) cultures. The ability of the primed cells to interfere with an optimal ovalbumin-specific PFC response in the target cultures was used as a measure of antigen-specific suppressor cell activity. The results demonstrated reduced suppressor cell activity in the SLE patients relative to controls--46.8 +/- 3.6% vs 63 +/- 2.4% suppression respectively (P less than 0.01). Consistent with reduced suppressor cell activity was an increase in the plaque-forming cell response to ovalbumin in patients relative to controls (880 +/- 73 vs 763 +/- 102 PFC/10(6) cells respectively [P = 0.10]). No correlation was demonstrated between suppressor cell activity in SLE patients and disease activity or therapy.

Adult↗

PubMed1980

Enhanced delayed hypersensitivity skin test reactivity with serial testing in healthy volunteers.

Delayed hypersensitivity skin testing with multiple antigens is frequently used to evaluate immunopotentiation therapy in man. Since serial skin testing with a single antigen has been shown to augment the skin test response, the present study was undertaken to assess the effect of serial delayed hypersensitivity skin testing on skin test reactivity with multiple antigens. Each of twelve healthy volunteers received 0.1 ml of five antigens on two occassion, 6 weeks apart. The antigens used were streptokinase-streptodornase, Candida, Trichophyton, mumps and Mycobacterium tuberculosis (PPD). The results demonstrated enhancement of skin test reactivity in the majority of tests. Indeed, 38.9% of the tests which were negative with the first skin test ( less than 10 mm induration) converted to positive. Enhancement in reactivity was observed in the majority of test subjects with all antigens except PPD. Similar enhanced skin test reactivity was observed in fifteen additional subjects tested serially with Candida only. The observations in this study suggest that uncontrolled studies of immunopotentiation must be interpreted with caution since serial delayed hypersensitivity skin testing with single or multiple antigens results in enhanced skin test reactivity.

Adult↗

PubMedDate not supplied

Musculoskeletal manifestations of infection with human immunodeficiency virus.

Musculoskeletal manifestations and autoimmune phenomena are recently recognized complications of infection with human immunodeficiency virus (HIV). Patients with HIV infection share several clinical and serologic features with patients with systemic lupus erythematosus and Sjögren's syndrome; these similarities may lead to diagnostic confusion. The significance of the presence of different types of autoantibodies in HIV-infected patients is still uncertain and may reflect polyclonal B cell activation. Musculoskeletal complications--particularly arthritis, arthralgia, and myalgia--are common in advanced stages of HIV infection. Clinical characteristics and serologic findings support the premise that most of the musculoskeletal complications (e.g., Reiter's syndrome, psoriatic arthritis, acquired immunodeficiency syndrome-associated arthritis and arthralgias) are reactive in nature--either a direct consequence of HIV infection or a result of various opportunistic infections. The possibility of HIV infection should be considered in all patients with conditions suggesting reactive arthritis. Further studies are needed to define the full spectrum and frequency of these musculoskeletal abnormalities.

Autoantibodies↗

PubMedDate not supplied

Isolated septic meningococcal arthritis.

Among the different types of arthritis associated with meningococcal disease, isolated primary meningococcal arthritis is unusual. A case of isolated group C meningococcal septic arthritis in an HIV negative homosexual male is described and its possible implication discussed.

Adult↗