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Biomedical subjects

D Gil

Publications and source records attributed to D Gil.

At least 37 records · Page 2Linked to original sources
PubMed1988

Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start.

The dicA1 mutation, located in the replication termination region of Escherichia coli at 34.9 min, confers a temperature-sensitive, division defective phenotype to its hosts. Previous analysis had suggested that dicA codes for a repressor of a nearby division inhibition gene dicB. We show now that gene dicB is part of a complex operon. Five open reading frames (ORFs 1 to 5) preceeded by a promoter sensitive to dicA repression are found within a 1500 bp segment, and are organized into two clusters separated by a long untranslated region. Evidence for expression of these ORFs was obtained from in vitro or in vivo translation of plasmid-coded genes. IPTG-dependent cell filamentation was obtained when either the entire or the C-terminal part of the fourth ORF was placed under control of the lac promoter. In both cases, a 7 KD protein corresponding to translation from an in-frame ATG of ORF4 (dicB) was made. We propose that this C-terminal protein is the division inhibitor synthesized in dicA1 mutants.

Amino Acid Sequence↗
PubMed1988

Four methods for general anaesthesia in the rabbit: a comparative study.

The efficacy and safety of pentobarbitone, ketamine/xylazine, fentanyl/fluanisone/diazepam, and halothane/nitrous oxide anaesthesia were compared in 4 groups of six New Zealand White rabbits. Heart and respiratory rates, body temperature, reflexes, blood pressure and blood gases were measured. Pentobarbitone appeared to be unsuitable for anaesthesia in rabbits, as 5 of the 6 rabbits to whom it was administered, required artificial respiration or died. The combinations of ketamine/xylazine and fentanyl-fluanisone/diazepam both produced unpredictable levels of anaesthesia together with a substantial decline in arterial blood pressure and PO2. Despite a severe drop in blood pressure (up to 37.5%), anaesthesia with halothane and nitrous oxide was found to be superior to the other anaesthetic agents.

Anesthesia, General↗
PubMed1986

[Simple surgical treatment of hip dislocation in dogs and cats using a modified toggle].

Surgical treatment of dislocations of the hip by a modified toggle as described by Knowles (1953) usually results in a very rapid and permanent restauration of function to normal. An essential feature of this method is the slight surgical injury. This is achieved by using a relatively small toggle and drills with small diameter. The surgical technique and the results of treatment in 18 cats and 10 dogs are reported.

Animals↗
PubMed1983

Differential diagnosis between amoebic liver abscess and acute cholecystitis.

The Research Committee of the World Organization of Gastroenterology has gather information regarding the etiology of acute abdominal pain. Seven diseases cover 96% of the causes of this syndrome in many countries of the world, but some geographical variations have been observed. One example of these variations is amoebic liver abscess, present in 5 to 10% of Mexico City patients. Right upper quadrant pain is often present in amoebic liver abscess and acute cholecystitis. Thus, differential diagnosis of these two entities is difficult. Using discriminant analysis and "stepwise" procedures in 100 cases with cholecystitis and a similar number of patients with amoebic liver abscess, we found six variables (symptoms and signs with a significant chi square to distinguish between these two diseases. The symptoms and signs chosen were hepatomegaly, Murphy's sign, duration of pain greater than or equal to 48 hours, previous history of abdominal pain, dysentery, and facial pallor. These variables proved to be better than laboratory test results. With five of these variables it was possible to obtain an accuracy of 92%. Using six variables, if cases of tie (three variables present and three absent) were excluded, accuracy rose to 96%.

Abdomen, Acute↗
PubMed1983

Choanal atresia. Report of seventeen cases.

Seventeen cases of choanal atresia are presented, of which 13 were female and 4 male. In 11 patients the malformation was bilateral, being unilateral in the remaining 6; 8 patients also presented other malformations amongst which esophageal atresia and congenital heart disease were the most common. Treatment is surgical, and the prognosis depends largely on the presence or absence of associated malformations.

Esophageal Atresia↗
PubMed1983

Action of (S)-10-hydroxycamptothecin on P388 leukemia and distribution of the drug in mice.

As an inhibitor of the growth of P388 leukemia in mice, (S)-10-hydroxycamptothecin (OPT) was as potent as the parent compound camptothecin (CPT). Incorporation of thymidine into DNA was the parameter most sensitive to OPT in vitro (ED50 approximately 4 micrograms/ml), but incorporation of cytidine into RNA and of acetate into lipids was also reduced significantly in the presence of the drug. The cytofluorometric profile suggested suppression of the S and G2/M phases. The distribution of OPT in mice at 2 and 24 hours after ip injection (10 mg/kg) was essentially similar to that of CPT, with the exception of a somewhat greater concentration of CPT in the liver. In their pharmacology, OPT and CPT appear to be very similar, despite reports that the hydroxy derivative is less toxic.

Animals↗
PubMed1978

Cheyletiellosis in long-haired cats.

Among 41 catteries, the majority of which were investigated because of problems of chronic pruritus, 27 proved to be infected by Cheyletiella mites. The skin lesions consisted of small erythematous papules with crusts and some loose hairs. After removal of the loose hairs Cheyletiella mites could be collected with adhesive tape for microscopic examination. Human involvement (papular urticaria) was observed in 20% of the cases. Bathing with Lindane was an effective mode of treatment.

Animals↗
PubMed1975

Unraveling child abuse.

This paper attempts to clarify the dynamics of child abuse, and to suggest approaches to primary prevention. Child abuse is redefined, within egalitarian value premises, as inflicted gaps in children's circumstances that prevent actualization of inherent potential. Levels of manifestation and causal dimensions of child abuse are identified, and their multiple interactions are traced. Primary prevention is shown to be essentially a political, rather than a purely technical or professional, issue.

Adolescent↗
PubMedDate not supplied

Hepatic angiomyolipoma: progressive changes in size and tumor composition.

We report on 8 years of imaging and clinical follow-up of a confirmed hepatic angiomyolipoma (AML) undergoing notable growth over this period. The tumor grew considerably in the first 5 years, and its growth especially affected the fatty component; in the last 3 years, growth occurred more slowly. Radiologists should be aware that tumor growth of a hepatic AML can occur.

Adult↗
PubMedDate not supplied

The pathobiology of ozone-induced damage.

Ozone remains one of the three most important air pollutants worldwide, yet little direct documented evidence of its genotoxicity exists. The interest in the pathology of ozone exposure and the molecular events that underlie its course stems from DNA damage caused by oxygen stress including hydroxyl radicals, superoxide, singlet oxygen, and hydrogen peroxide. Although the tissue damage associated with ozone inhalation occurs at both the conducting airway and the alveolus, the cellular and mechanistic processes underlying these events are less well understood. Ozone leads to the oxidative decomposition of polyunsaturated fatty acids. Ozone also depresses DNA replication in V79 Chinese hamster lung fibroblasts in a dose-dependent fashion (concentration, 1-10 ppm), which indicates that ozone or its reaction products may interact directly with DNA and inhibit replication. Ozone also linearizes circular DNA and induces ozone-sensitive mutant and pneumocytes to repair its DNA. DNA adducts have been implicated in aging, cellular transformation, mutagenesis, carcinogenesis, and cell death; DNA adducts are products of free radical damage. These events are all common in ozone exposure. Finally, DNA-binding proteins are potent positive and negative regulators, enhancers, or silencers of gene expression. Part of their action may be related to their ability to initiate the binding sequence of DNA transcription proteins and thus form complexes. Alteration of DNA-binding sites by ozone adducts may effect mRNA transcription due to altered binding by DNA-binding proteins. This altered transcription has been shown to effect growth factors involved in collagen and matrix regulation. The present review will address some of the complexities involved in ozone exposure.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

[Influence of tumor location in patients with breast cancer on the sentinel node detection].

OBJECTIVE: To evaluate the influence of tumour quadrant localization on the sentinel node (SN) detection and the visualisation of internal mammary chain (IM) drainage by radioisotopic techniques. 316 patients with breast cancer were studied. Mean age 57 years (range 29-88). All patients received 37-74 MBq of 99mTc-albumin nanocolloid in 2 ml by peritumoral injection. The breast cancer was located in the upper outer quadrant in 189 patients, in the upper inner in 57, in the lower outer in 57, in the lower inner in 55 and in the subareolar area in 18 patients. At two hours p.i., anterior and lateral chest lymphographies were obtained. The SN location was marked on the patient skin with permanent ink. SN was identified intraoperatively by the gamma probe. Histopatological analysis included imprints, delayed hematoxilin-eosin, inmunohistochemistry CAM 19-2 and PCR. RESULTS: The scintigraphy and surgical detection was in the upper outer quadrant of 90 % and 93 % respectively; in the lower outer quadrant of 91 % and 95 %, in the upper inner quadrant of 93 % and 95 %, in the lower inner quadrant 87 % and 95 % and in the subareolar area in 94 % and 83 %. The IM chain drainage was of 6 % in the UO, in the LO of 5 %, in the UI of 12 %, in the LI of 20 % and none in subareolar. CONCLUSIONS: Our data suggest that sentinel node location (quadrant) is not a influential factor in the scintigraphy and surgical detection. Tumours localised in internal quadrant show a higher rate of IM chain drainage.

Adult↗