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Biomedical subjects

D Gekle

Publications and source records attributed to D Gekle.

At least 19 recordsLinked to original sources
PubMed1991

Microheterogeneity of urinary albumin and tubular proteinuria in juvenile diabetes mellitus.

We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, beta 2-microglobulin and molecular size distribution of urinary proteins were measured, the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein (P less than 0.05) and beta 2-microglobulin excretion (P less than 0.01) were elevated. It is suggested that the appearance of highly heterogenous albumin in the pI range of 5.3-5.9 is the result of a decreased tubular reabsorption.

Adolescent↗
PubMed1985

Low molecular weight proteinuria in diabetic children--a marker of early diabetic nephropathy?

Twenty-four hour urine specimens of 67 diabetic children aged 1-17 years without any renal manifestations were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The excretion of high molecular weight, i.e. glomerular proteins was compared to that of low molecular weight, i.e. tubular proteins corresponding to more or less than 68,000 daltons. The glomerulo-tubular protein ratio (GTPR) obtained was significantly lower in diabetic patients compared with 30 healthy children of the same age and showed a linear decrease with longer duration of diabetes.

Adolescent↗
PubMed1979

[Molecular weight analysis of physiological proteinuria in newborn infants (author's transl)].

The physiological protien and glycoprotein excretions in the urine samples of a larger group of newborn infants were separated according to the molecular weights by SDS polyacrylamide gel electrophoresis and compared with the protein excretions of older children. We found higher proportions of albumin, of high molecular weight (MW = molecular weight greater than or equal to 150 000 dt) and of lower molecular weight (MW less than albumin 6800 dt) proteins in the first 24-h urine samples after birth. One week after birth the low molecular weight proteins predominated because there was a substantial decrease in the excretion of albumin and of high molecular weight proteins (MW greater than or equal to 150 000 dt). We compared the patterns of protein excretion of the newborn infants with those of children aged from 2 1/2 to 15 years. These urines samples showed a typical pattern of protein excretion not correlated to the age. These findings express a transitory immaturity of the glomerular filter and of the tubular protein reabsorbing system of the newborn kidney. Apparently, the tubular protein handling normalizes later than the glomerular filtration of proteins.

Adolescent↗
PubMed1977

[Lysozyme and beta2-microglobulin in cerebrospinal fluids from healthy children and in children with diseases of the central nervous system (author's transl)].

Lysozyme is absent from normal cerebrospinal fluid (C.S.F.) and in C.S.F. from children with viral meningitis. Appreciable amounts of lysozyme were noted in C.S.F. from children with bacterial meningitis (0.23 +/- 0.14 mg/100 ml) and cerebral convulsions (0-0.82 mg/100 ml). The C.S.F.-lysozyme content is a sensitive indicator for bacterial meningitis and important in the differential diagnosis between viral and bacterial meningitis. The beta2-microglobulin content of C.S.F. in healthy children was 0.11 +/- 0.05 mg/100 ml; in children with viral meningitis 0.20 +/- 0.06 mg/100 ml and in children with bacterial meningitis 0.44 +/- 0.17 mg/100 ml. Children with cerebral convulsions had also a rise in C.S.F. beta2-microglobulin.

Adolescent↗
PubMed1977

[Ifosfamide in the treatment of nephrotic syndrome (author's transl)].

Ifosfamide, an alkylating agent was used successfully in the treatment of children with steroid sensitive nephrotic syndrome with minimal changes. Remissions have persisted for about 6 years. Toxicity was very minor, and the regimen constitutes a useful advance in management of these patients. Ifosfamide had no significant effect on children with steroid resistence nephrotic syndrome or other morphological changes. Because the potential gonadal dysfunction Ifosfamide should be given only in patients with steroid toxicity or frequent relapsers.

Adolescent↗
PubMed1977

[Changing of clinical symptoms in glomerulonephritis (author's transl)].

76 children with glomerulonephritis (biopsy diagnosed) were studied for evidence of preceeding streptococcal infection, morphological and clinical symptoms. Glomerulonephritis often shows minimal symptoms; only 11% had acute, but 80% subclinical symptoms. Heading symptom was hematuria (95%), in 55% there was additional proteinuria. No correlation could be found between the clinical symptoms and glomerular lesions. Antistreptolysin 0 titers were elevated in only 39% of the children, but in all with acute clinical symptoms. The aetiology of glomerulonephritis in the remainder is uncertain, probably virus infections. The reason for decreased nephritis and increased subclinical nephritis is due to penicillin therapy and the improved renal diagnosis (renal biopsy).

Adolescent↗
PubMed1976

[Kidney anomalies in Ullrich-Turner-syndrome (author's transl)].

Intravenous urography was performed in fourteen children with Ullrich-Turners syndrome. Renal abnormalities have been noted in twelve cases (85,7%). The most frequent kidney anomalies were malrotations (28,5%), horseshoe kidneys (21,4%) and double kidneys (21,4%). Malformations of kidney are thus a very frequent feature in Ullrich-Turners syndrome. It is therefore recommendable to perform in any case of Ullrich-Turners syndrome an intravenous urography, since these abnormalities are clinically latent.

Adolescent↗
PubMed1975

[Investigations on the Heredity of the Nephrotic Syndrome (author's transl)].

The familial nephrotic syndrome has a frequency of 3%. There are 2 types of manifestation. A malignant form with probably autosomal recessive inheritance and bad prognosis, and a benign form with the histology of mimal change disease, complete recovery and a multifactorial inheritance. According to the literature and our own calculations there is in the BRD a yearly frequency of 9--14.4 families with a familial nephrotic syndrome, based on the assumption of 300--480 new cases of nephrotic syndrome per year.

Age Factors↗
PubMed1975

[beta2-Microglobulin in healthy children and in children with renal diseases (author's transl)].

beta2 microglobulin is a plasma protein of low molecular weight (11800), which also appears in urine in small quantities and cerebrospinal fluid. Its serum concentration in healthy children is 0.12 plus or minus 0.04 mg/100 ml, its renal excretion is 0.07 plus or minus 0.05 mg/24 hrs. In glomerular nephropathy serum levels are augmented with diminished GFR, while in tubular nephropathy excretion in the urine is raised considerably. There is a close correlation between serum beta2 microglobulin and GFR.

Acute Disease↗