Biomedical subjects

D Gao

Publications and source records attributed to D Gao.

At least 55 records · Page 3Linked to original sources

PubMed2000

Interactions of intrathecally administered ziconotide, a selective blocker of neuronal N-type voltage-sensitive calcium channels, with morphine on nociception in rats.

Ziconotide is a selective, potent and reversible blocker of neuronal N-type voltage-sensitive calcium channels (VSCCs). Morphine is an agonist of mu-opioid receptors and inhibits N-type VSCC channels via a G-protein coupling mechanism. Both agents are antinociceptive when they are administered intrathecally (spinally). The present study investigated the acute and chronic (7-day) interactions of intrathecally administered ziconotide and morphine on nociception in several animal models of pain. In the acute study, intrathecal bolus injections of morphine and ziconotide alone produced dose-dependent inhibition of formalin-induced tonic flinch responses and withdrawal responses to paw pressure. The combination of ziconotide and morphine produced an additive inhibition of formalin-induced tonic flinch responses and a significant leftward shift of the morphine dose-response curve in the paw pressure test. After chronic (7-day) intrathecal infusion, ziconotide enhanced morphine analgesia in the formalin test. In contrast, chronic intrathecal morphine infusion produced tolerance to analgesia, but did not affect ziconotide antinociception. Antinociception produced by ziconotide alone was the same as that observed when the compound was co-administered with morphine to morphine-tolerant rats. In the hot-plate and tail immersion tests, chronic intrathecal infusion of morphine lead to rapid tolerance whereas ziconotide produced sustained analgesia with no loss of potency throughout the infusion period. Although ziconotide in combination with morphine produced an apparent synergistic analgesic effects during the initial phase of continuous infusion, it did not prevent morphine tolerance to analgesia. These results demonstrate that (1) acute intrathecal administrations of ziconotide and morphine produce additive or synergistic analgesic effects; (2) chronic intrathecal morphine infusion results in tolerance to analgesia but does not produce cross-tolerance to ziconotide; (3) chronic intrathecal ziconotide administration produces neither tolerance nor cross-tolerance to morphine analgesia; (4) intrathecal ziconotide does not prevent or reverse morphine tolerance.

Analgesics, Opioid↗

PubMed2000

Reaction of beta-alkannin (shikonin) with reactive oxygen species: detection of beta-alkannin free radicals.

beta-Alkannin (shikonin), a compound isolated from the root of Lithospermum erythrorhizon Siebold Zucc., has been used as a purple dye in ancient Japan and is known to exert an anti-inflammatory activity. This study aimed to understand the biological activity in terms of physico-chemical characteristics of beta-alkannin. Several physico-chemical properties including proton dissociation constants, half-wave potentials and molecular orbital energy of beta-alkannin were elucidated. This compound shows highly efficient antioxidative activities against several types of reactive oxygen species (ROS), such as singlet oxygen ((1)O2). superoxide anion radical (.O2), hydroxyl radical (.OH) and tert-butyl peroxyl radical (BuOO.) as well as iron-dependent microsomal lipid peroxidation. During the reactions of beta-alkannin with 1O2, .O2- and BuOO., intermediate organic radicals due to beta-alkannin were detectable by ESR spectrometry. Compared with the radicals due to naphthazarin, the structural skeleton of beta-alkannin, the beta-alkannin radical observed as an intermediate in the reactions with (1)O2, and .O2- was concluded to be a semiquinone radical. On the other hand, during the reactions of beta-alkannin and naphthazarin with BuOO., ESR spectra different from the semiquinone radical were observed, and proposed to result from the abstraction of hydrogen atoms from phenolic hydroxyl groups of beta-alkannin by BuOO.. Based on the ROS-scavenging abilities of beta-alkannin, the compound was concluded to react directly with ROS and exhibits antioxidative activity, which in turn exerts anti-inflammatory activity.

Acetonitriles↗

PubMed2000

Analysis of the methionine biosynthetic pathway in the extremely thermophilic eubacterium Thermus thermophilus.

Four DNA fragments that could rescue the mutations of four Met- mutants were cloned from Thermus thermophilus HB27 and their complete nucleotide sequences were determined. Two of the four fragments respectively contained the greater parts of the metF and metH genes, the predicted amino acid sequences of which showed identities of 30.8% and 32.7% with 5,10-methylenetetrahydrofolate reductase (EC 1.7.99.5) and vitamin B12-dependent homocysteine transmethylase (EC 2.1.1.13) of Escherichia coli. The other two DNA fragments, which overlapped one another, contained two open reading frames whose predicted amino acid sequences were respectively similar to those of O-acetylhomoserine sulfhydrylase (EC 4.2.99.10, the product of the MET17 gene) and homoserine O-acetyltransferase (EC 2.3.1.31, the product of the MET2 gene) of Saccharomyces cerevisiae. The metF, metH, MET2, and MET17 genes of T. thermophilus were disrupted by introducing the heat-stable kanamycin nucleotidyltransferase gene into the genome. Each transformant showed methionine auxotrophy. Both the MET2- and MET17-disrupted mutants could grow in a minimal medium containing homocysteine but not in the same medium containing succinylhomoserine or cystathionine. In contrast, the metF- and metH-disrupted mutants could not grow in the minimal medium containing homocysteine. These results suggest that in T. thermophilus, homoserine is directly converted to homocysteine via O-acetylhomoserine and that homocysteine is methylated to synthesize methionine.

Journal Article↗

PubMed2000

Different costimulation signals used by CD4(+) and CD8(+) cells that independently initiate rejection of allogenic hepatocytes in mice.

The current study evaluated the role of CD40/CD40 ligand (CD40L) and CD28/B7 costimulation signals during alloimmune responses independently mediated by CD4(+) or CD8(+) T cells. Allogeneic hepatocytes were transplanted into CD8 or CD4 knock out (KO) mice under cover of costimulatory blockade. Rejection of FVB/N (H-2(q)) hepatocytes occurred by day 10 posttransplant in untreated CD8 or CD4 KO (H-2(b)) mice. Treatment of CD8 or CD4 KO mice with anti-CD40L monoclonal antibody (mAb; MR1) resulted in significant prolongation of hepatocyte survival indicating that CD40/CD40L interactions were critical in both CD4(+) and CD8(+) T-cell initiated hepatocyte rejection. Anti-CD40L mAb also prolonged hepatocyte survival in B-cell KO (H-2(b)) mice, indicating that the efficacy of CD40/CD40L blockade in preventing hepatocyte rejection was B-cell (and antibody) independent. In contrast, treatment with CTLA4 fusion protein (CTLA4Ig), prolonged hepatocyte survival in CD8 KO but not CD4 KO mice, showing that CD28/B7 interactions were important in CD4(+) but not CD8(+) T-cell initiated hepatocyte rejection. Under selected circumstances, such as in CD40 KO mice, both CD4(+) and CD8(+) T cells mediate hepatocyte rejection in the absence of CD40/CD40L costimulation and without a significant contribution from CD28/B7 costimulation signals. These results highlight the disparate roles of CD40/CD40L and CD28/B7 costimulation signals in CD4(+) versus CD8(+) T-cell mediated immune responses to allogeneic hepatocytes. The CD4(+) T-cell independent, CD40L-sensitive, CD28/B7-independent pathway of CD8(+) T-cell activation in response to transplantation antigens is novel.

Abatacept↗

PubMed2000

Mechanisms of cryoinjury in living cells.

Biological metabolism in living cells dramatically diminishes at low temperatures, a fact that permits the long-term preservation of living cells and tissues for either scientific research or many medical and industrial applications (e.g., blood transfusion, bone marrow transplantation, artificial insemination, in vitro fertilization, food storage). However, there is an apparent contradiction between the concept of preservation and experimental findings that living cells can be damaged by the cryopreservation process itself. The challenge to cells during freezing is not their ability to endure storage at very low temperatures (less than -180 degrees C); rather, it is the lethality of an intermediate zone of temperature (-15 to -60 degrees C) that a cell must traverse twice--once during cooling and once during warming. Cryobiological research studies the underlying physical and biological factors affecting survival of cells at low temperatures (during the cooling and warming processes). These factors and mechanisms (or hypotheses) of cryoinjury and its prevention are reviewed and discussed, including the most famous two-factor hypothesis theory of Peter Mazur, concepts of cold shock, vitrification, cryoprotective agens (CPAs), lethal intracellular ice formation, osmotic injury during the addition/removal of CPAs and during the cooling/warming process, as well as modeling/methods in the cryobiological research.

Animals↗

PubMed2000

Decoy oligodeoxynucleotide characterization of transcription factors controlling endothelin-B receptor expression in vascular smooth muscle cells.

Endothelin-1 is not only a powerful vasoconstrictor but also a potent mitogen for vascular smooth muscle cells (SMC), acting through both the endothelin-A and endothelin-B receptor (ET(B)-R). Although vascular SMC are known to express the ET(B)-R, its transcriptional regulation has not been studied thus far. Here we demonstrate that the potent inhibitor of nuclear factor kappaB activation, pyrrolidine dithiocarbamate (PDTC; 30-100 microM), induces de novo ET(B)-R expression in rat aortic and mesenteric cultured SMC. Electrophoretic mobility shift analyses revealed that besides inhibition of nuclear factor kappaB, PDTC enhances activator protein-1 (AP-1), CCAAT/enhancer-binding protein (C/EBP), and GATA-2 activity in these cells. Preincubation of PDTC-stimulated cells with appropriate decoy oligodeoxynucleotides confirmed the involvement of these three transcription factors, namely that of AP-1, in ET(B)-R expression. The stimulatory effect of PDTC on ET(B)-R expression was also confirmed functionally by monitoring an enhanced ET-1-induced apoptosis in PDTC-treated cells that was sensitive to the ET(B)-R antagonist, BQ788. Taken together, these findings demonstrate that C/EBP, GATA-2, and in particular AP-1 can control ET(B)-R expression in vascular SMC. They further support the notion that ET(B)-R expression in these cells may play an important role in cardiovascular complications, such as restenosis following angioplasty that in the early phase is characterized by prominent SMC apoptosis.

Animals↗

PubMed2000

Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet.

Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridization of genomic DNA, six clones were determined to represent highly repetitive DNA families present only in the B. corolliflora genome. Five other sequences were highly repetitive in B. corolliflora and low or single copy in B. vulgaris. The insert size varied between 43 bp and 448 bp. Two sequences pBC1279 and pBC1944 displayed strong homology to a previously cloned satellite DNA from B. nana. With one exception, sequences are tandemly arranged as revealed by a typical ladder pattern after genomic Southern hybridization. The chromosomal distribution of five probes was determined by fluorescence in situ hybridization (FISH) of mitotic metaphases from B. corolliflora and a triploid hybrid between B. vulgaris and B. corolliflora. Three sequences were spread along all chromosome arms of B. corolliflora while one sequence was present on only six chromosomes. The chromosome-specific sequence pBC216 was found in close vicinity to the 5S rDNA located on B. corolliflora chromosome IV. This set of species-specific sequences has the potential to be used as probes for the identification of monosomic alien addition lines and for marker-assisted gene transfer from wild beet to cultivated beet.

Base Sequence↗

PubMed2000

Losartan reduces collagen content and intimal thickening of iliac arteries after balloon injury in rabbits.

Intimal thickening and formation of extracellular matrix are parts of the repair process after vascular injury. Similar processes occur after coronary angioplasty. Prior studies have shown that losartan inhibits intimal thickening in rat carotid arteries following balloon injury. However, the effects of losartan in reducing the collagen content of arteries after balloon injury have not been examined. The objectives of this study were to determine the change in collagen content after balloon injury and to analyse the mechanisms of reduction of collagen content and intimal thickening. Losartan (15 mg/kg/d) was administered orally from six days before to eight weeks after balloon injury in rabbits. Collagen content was measured histologically by the use of circularly polarised images of picrosirius red-stained sections. Collagen content in arterial intima was found to be significantly lower in the losartan-treated group (n= 12) than in the control group (n= 12)(21.6% +/- 5.2% vs. 43.8% +/- 7.6%, p<0.01). Losartanreduced the collagen content in arterial intima by 50.7% area fraction compared with that of control. The morphological observation showed that the intimal area and intimal-to-medial area ratio in the losartan-treated group were significantly less than in the control group (0.27 +/- 0.13mm2 vs. 0.52 +/- 0.29 mm2,0.55 +/- 0.21 vs. 0.97 +/- 0.25, respectively, p<0.05). These data indicate that losartan reduces vascular collagen content and inhibits intimal thickening after balloon injury. The results also suggest that collagen accumulation in the intima may be an important factor in the development of the stenotic lesion and that the use of losartan may have therapeutic value to prevent stenosis after balloon injury.

Angiotensin II↗

PubMed2000

Lysophosphatidylcholine enhances superoxide anions production via endothelial NADH/NADPH oxidase.

Reactive oxygen species (ROS) including superoxide anions (O2(-)) play a key role in atherogenesis, and endothelial cells have the ability to generate ROS. To investigate the enzymatic sources of ROS and the effects of lysophosphatidylcholine (LPC), an atherogenic lipid, we measured ROS production in cultured bovine aortic endothelial cells (BAECs) by the lucigenin-enhanced chemiluminescence (CL) method and electron spin resonance (ESR). BAEC homogenates had the enzymatic activity of NADH/NADPH oxidase. BAECs cultured on microcarrier beads generated O2(-) under basal conditions. The inhibition of NADH/ NADPH oxidase by diphenylene iodonium (DPI) significantly attenuated O2(-) production, whereas no inhibitors of other oxidases suppressed it. Although LPC enhanced O2(-) production approximately 3.1-fold, its action was suppressed by DPI. Tyrosine kinase inhibitors significantly attenuated LPC-induced O2(-) production. ESR with DMPO demonstrated that LPC increased the formation of the DMPO-hydroxyl adduct in dose- and time-dependent manners. These data suggest that the basal production of O2(-) in endothelial cells is mainly mediated by the NADH/NADPH oxidase system and that LPC activates this oxidase to enhance O2(-) production through a tyrosine kinase-dependent pathway. The enhancement of ROS production by LPC is probably involved in its atherogenic property.

Acridines↗

PubMed2000

[Alpha-acetolactate decarboxylase from B. licheniformis AS10106: cloning and expression gene in E. coli and S. cerevisiae].

A genomic library of B. licheniformis AS10106 that contained the alpha-acetolactate decarboxylase gene(alpha-ALDC) was constructed with vector pUC19 and host E. coli JM109 strain. The inserted fragments of foreign DNA ranged from 4 to 10 kb in the 4800 clones thus obtained. Six positive clones were detected after screening the plated library by the method of clony coloration. Subcloning of the DNA fragment containing the alpha-acetolactate decarboxylase gene showed that the alpha-acetolactate decarboxylase gene was on an 1.6 kb BamH I-EcoR I fragment. Preliminary analysis of the enzyme expressed from one recombinant plasmid pGEA showed that the properties of the recombinant enzyme, such as the optimal temperature and pH of reaction, were identical to those of the native enzyme. Using yeast-E. coli shuttle vector pYES2, an expression recombinant plasmid pYEA containing B. licheniformis AS10106 alpha-acetolactate decarboxylase gene was constructed. S. cerevisiae H158 transformed with pYEA had expressed alpha-acetolactate decarboxylase activity and shown the ability to reduce the formation of diacetyl during beer fermentation.

Carboxy-Lyases↗

PubMed2000

Changes in the firing pattern of globus pallidus neurons after the degeneration of nigrostriatal pathway are mediated by the subthalamic nucleus in the rat.

Changes in the neuronal activity of globus pallidus (GP) have been shown in animal models of parkinsonism. In order to study the implication of the subthalamic nucleus (STN) in these changes, the effects of STN lesions alone or in combination with 6-hydroxydopamine (6-OHDA) -induced damage to the substantia nigra compacta (SNc) were examined in rats using electrophysiological recordings of GP cells. In normal rats, the firing rate was 22.1+/-1.4 spikes/s. The pattern was regular in 45%, irregular in 49% and bursty in 6% of the cases. In rats with STN lesions, the firing rate of GP units (20.15+/-1.25 spikes/s) did not differ from that of normal rats and only regular (46%) and irregular (54%) cells were found; a bursty pattern was not observed. 6-OHDA lesions of the SNc induced no change in the firing rate of GP neurons (21.5+/-1.4 spikes/s, P>0.05) but a significant decrease in the percentage of regular cells (27%, P<0.001), a significant increase in burst cells (21%, P<0.001) with no change in the percentage of irregular units (52%) were observed. In rats with combined SNc and STN lesions, the firing pattern did not change from that of normal rats. The present results show that STN lesions induced the disappearance of bursts in normal rats and normalization of firing pattern in the GP units of rats with 6-OHDA lesions suggesting that the STN plays an important role in the modulation of the pattern of activity of GP neurons which may account for the therapeutic effect of STN lesions in Parkinson's disease.

Animals↗

PubMed2000

[Antitumor activities in vivo of interleukin-12 gene modified dendritic cells in murine models].

OBJECTIVE: To study the antitumor activities of the mIL-12 transfected and acid-eluted peptide sensitized dendritic cells (DC) in murine H22 liver cancer model. METHODS: The murine bone marrow derived DC were transfected with the recombinant adenovirus (Ad mIL-12) containing the mIL-12 gene and control virus AdBGFP. The peptides on the surface of the murine H22 cell line were eluted with mild acid buffer and then sensitized the transfected DC. The experimental animals were immunized with the differently disposed DC and the state of tumorgenesis and tumor growth was observed. RESULTS: The tumor growth and weight in the group immunized with mIL-12 transfected and acid-eluted peptide sensitized DC were significantly lower than those of control group (P<0.05). CONCLUSION: The mIL-12 transfected and acid-eluted peptide sensitized DC can induce obvious antitumor activities in the murine liver cancer models and may suggest new strategies for constructing new type of DC vaccine for liver cancer.

Animals↗

PubMed2000

[Inhibition of human glioma growth in nude mice by local secretion of angiostatin K(1-3)].

OBJECTIVE: To discuss the feasibility of human glioma therapy by the local secretion of angiostatin K(1-3)[AK(1-3)]. METHOD: AK(1-3) cDNA with secretive signal was inserted into polylinker sites of eukaryotic expression vector pcDNA3 to construct pcDNA-SAK(1-3); The vector was transfected into human glioma SHG44 cells by lipofectamine and the positive clone was screened by G418. The biological characters of glioma cells were examined with electron microscopy and FCM. The activity of AK(1-3) protein expressed by the SHG44 cells was examined by the endotheliocyte inhibition assay and immunofluorescence assay. When the tumor cells were implanted into nude mice, the tumor necrosis and micrangium was calculated by immunohistochemistry and electron microscopy in order to determine the influence of AK(1-3) protein to the human glioma growth. RESULTS: The pcDNA-SAK(1-3) vector was successfully constructed and transfected into glioma cells that could express AK(1-3) protein. The tumorigenesis and angiogenesis of glioma cells in nude mice were greatly reduced (tumour end volume, experiment group 170 mm3, control group 8 120 mm3, P < 0.01; Blood vessel counting, experimental group 5.4, control group 12.2, P < 0.01). CONCLUSION: The human glioma angiogenesis and growth were inhibited by the local secretion of AK(1-3). It can be further used in the treatment of other solid tumors.

Angiostatins↗

PubMed2000

[Application of polyester fiber heart patches to secondary intraorbital implantation of hydroxyapatite spheres].

OBJECTIVE: To observe the effect of intraorbital implantation of hydroxyapatite spheres wrapped with polyester fiber heart patches. METHOD: According to the rectus contraction points four rectus were found and the rectus valves were created in anophthalmos. Then the hydroxyapatite sphere wrapped by the polyester fiber heart patch was inserted in the orbit. RESULT: There were 13 cases in this group. Of them 11 patients showed very good operative results, Local poor healing of bulbar conjunctiva and fascia occurred in 2 patients. The wound in one patient healed very well after a simple repairing operation and in the other the wound healed spontaneously without any special treatments. CONCLUSION: The effect of secondary intraorbital implantation of hydroxyapatite spheres wrapped by polyester fiber heart patches is positive. The polyester fiber heart patch is safe, reliable, cheap, very convenient in use and easy to obtain. It is a better substitute for sclera which is used to wrap the HA sphere.

Adult↗

PubMed2000

Diagnostic potential of mitochondrial DNA assessment in patients with optic neuropathy.

OBJECTIVE: To study the primary mutations of mitochondrial DNA (mtDNA) associated with Leber's hereditary optic neuropathy (LHON) in patients with optic neuropathy. METHODS: Seventy-nine patients with a variety of bilateral optic neuropathies were examined. Mutations at np3460, np11,778 and np14,484 of mtDNA were tested by PCR-restriction detection in peripheral blood DNA from 16 cases of clinically probable LHON, 44 cases of possible LHON, 2 cases of alcohol amblyopia, 4 cases of multiple sclerosis, 5 cases of autosomal dominant optic atrophy, 4 cases of primary open-angle glaucoma, 3 cases of spinocerebellar degeneration, and 1 case of ethambutol-induced optic neuropathy. RESULTS: The mutation at np11778 was identified in 31 cases (39.2%) to establish LHON, which consisted of: all 16 of clinically probable LHON cases, 13 cases (29.5%) of possible LHON, and 2 cases of alcohol amblyopia. The remaining 48 cases were negative for mtDNA mutations at np3460, np11 778, and np14,484. CONCLUSION: Assessment of mtDNA provides a useful diagnostic aid in the definition and exclusion of LHON, in particular family history-negative, otherwise undefined bilateral optic nerve inflammatory disease.

Adolescent↗

PubMed2000

Angiostatin K(1-3) gene for treatment of human gliomas: an experimental study.

OBJECTIVE: To discuss the feasibility of gene therapy of human glioma by antiangiogenesis method. METHODS: Angiostatin K(1-3) cDNA with secretive signal was inserted into the polylinker sites of eukaryotic expression vector pcDNA3 to construct pcDNA-SAK(1-3). The vector was transfected into human SHG44 glioma cells by lipofectamine and the positive clone was screened by G418. The biological characteristics of glioma cells were examined by electronmicroscope and flow cytometry. The activity of angiostatin K(1-3) protein expressed by SHG44 cells was examined by the bovine micrangium endotheliocyte inhibition assay and immunofluorescence assay. When SHG44 cells were implanted into the strata subcutaneum of nude mice, tumor necrosis and micrangium were calculated immunohistochemically and electronmicroscopically for determining their characteristics and validity in gene therapy of human glioma by antiangiogenesis method. RESULTS: The eukaryotic expression vector pcDNA-SAK (1-3) was successfully constructed and transfected into glioma cells. The cells expressed angiostatin K(1-3) protein, and their tumorigenesis and angiogenesis in nude mice were greatly reduced. CONCLUSION: Angiostatin K(1-3) gene is feasible to treat human glioma. This experiment lays a foundation for gene therapy of the other solid tumors by antiangiogenesis method.

Angiostatins↗

PubMed2000

[Clinical evaluation of glutaldehyde resinifying therapy on pulp diseases].

OBJECTIVE: To evaluate clinically the glutadehyde resinifying therapy(GR) on pulp diseases. METHODS: A total of 270 teeth with pulp diseases were divided into GR and formaldehyde resinifying therapy(FR) groups. The latter served as a control. Immediate post-operation reactions, short-term (0.5-1 year) and long-term (2-4 years) results were compared between the two groups. RESULTS: Ratio of post-operation pain for GR group was 10.2%, while 19.8% for FR group. The difference was statistically significant (P < 0.05, by chi 2 test). The short-term successful rates were 85.4% and 84.9% for GR and FR groups, respectively, whilst the long-term rates were 92.0% and 90.8%, respectively, there were no significant differences between the two groups statistically. CONCLUSIONS: GR could obtain similar clinical results as FR, and has an advantage of lower rate of post-operation pain compared with FR.

Adolescent↗

PubMed2000

[Effect of abdominal lavage with Chinese drugs on bacteria translocation in acute hemorrhagic necrotizing pancreatitis in rats].

OBJECTIVE: To investigate the effect of different abdominal lavage fluid on bacterial translocation in acute hemorrhagic necrotizing pancreatitis (AHNP) of rat model. METHODS: One hundred and sixty Wistar rats were randomly divided into the control group, AHNP group, antibiotics group and Chinese drugs group. The treated group were treated with intraperitoneal lavage for 72 hours. The peritoneal fluid and blood samples were collected for bacterial culture. Pancreas were examined histopathologically. RESULTS: In Chinese drugs group, the pathologic damage in pancreas were milder than that of AHNP group, and as compared with AHNP group, the bacterial positive rates of blood and peritoneal lavage fluid culture were reduced from 80% to 40% and 90% to 40% respectively. CONCLUSION: AHNP impaired the gut barrier function which led to bacterial translocation from the gut to other organs. It plays an important role in intestinal infection secondary to AHNP. Abdominal lavage with Chinese drugs is effective in preventing intestinal bacterial translocation, it showed its protection on gut barrier function by alleviating the damage of intestinal mucosa and pancreatitis.

Animals↗