Search PubMed⌕ Search

Biomedical subjects

D F Brown

Publications and source records attributed to D F Brown.

At least 109 records · Page 6Linked to original sources

Dental health promotion in a group of children at high risk to dental disease.

Three hundred and ten children from three schools were examined before and after a dental health promotion program targeted at one of the schools. Parents of subjects at the experimental school were visited at home by a community health worker who provided individualized information on dental services and preventive strategies. Parents of subjects at the control school were provided with similar information by way of general written documentation, not tailored to specific needs, and distributed as part of the traditional school take home system. The experimental hypothesis proposed that, at re-examination, subjects from the experimental school would show a measurable improvement in caries increment, and a reduced plaque index over subjects from the control schools. Although the individualized social intervention strategy did not demonstrate a major shift in use of dental services or improvement in health status some improvement in caries experience was obtained in the target group. The multifactorial nature of dental disease and the limited contact of the health worker with the families involved would account for the low success rate of this strategy.

Australia↗

Antimicrobial susceptibility testing of Haemophilus influenzae: trial organised as part of United Kingdom national external quality assessment scheme for microbiology.

Six strains of Haemophilus influenzae were distributed to 417 United Kingdom laboratories who were asked to test susceptibility of the strains to ampicillin, augmentin, tetracycline, chloramphenicol, and trimethoprim and to test for beta lactamase production. Laboratories were also asked to provide details of their methods by completing a questionnaire. The incidence of reports recording sensitive strains as resistant was 8% (ampicillin), 7% (augmentin), 3% (tetracycline), 1% (chloramphenicol), and 12% (trimethoprim). The incidence of reports recording resistant strains as sensitive was 9% (ampicillin), (2% with beta lactamase producing strains, 24% with non-beta lactamase producing strains), 51% (augmentin), 10% (tetracycline), 20% (chloramphenicol), and 3% (trimethoprim). High error rates were associated with several methods or practices. These included use of general purpose growth media rather than susceptibility testing media and failure to add lysed blood to the media when testing trimethoprim susceptibility; standardise the inoculum; use suitable control strains; and the use of high content discs for testing chloramphenicol, tetracycline, and ampicillin.

Amoxicillin↗

Naloxone fails to reverse hemorrhagic hypotension.

The effect of opiate antagonist therapy with naloxone on hemorrhagic shock was examined. An equivalent intravenous bolus of naloxone or normal saline had the same pressor effect in conscious, unrestrained, hypovolemic hypotensive rats. The proposal that opiate antagonists are of therapeutic value in the treatment of hemorrhage is questioned.

Animals↗

Penicillin-binding proteins of beta-lactam-resistant strains of Staphylococcus aureus. Effect of growth conditions.

Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains with a low affinity for beta-lactams and that its amount is regulated by the growth conditions. The new PBP from all strains that have been examined has an identical mobility on SDS gel electrophoresis and is the only PBP still present in an uncomplexed state with beta-lactams (and therefore the only functional PBP when these strains are grown in media containing concentrations of beta-lactam antibiotics sufficient to kill sensitive strains.

Anti-Bacterial Agents↗

The effect of WR 149024 on survival following hemorrhage in conscious rats.

The effect of WR 149024, an alpha-receptor antagonist, on survival following hemorrhage with no volume replacement therapy, was investigated. WR 149024 (either 5.0 or 50.0 mg/kg) was administered intraperitoneally (i.p.) to conscious rats during exsanguination. Both WR 149024 treatments caused a significant decrease in survival (P less than 0.021), compared with water-injected controls.

Adrenergic alpha-Antagonists↗

Customized vascular catheters for rodents.

Custom arterial and venous catheters were made for rodents from polyurethane tubing. The low thrombogenicity and toxicity index, chemical stability and resiliency of polyurethane made this tubing an ideal catheter material. The tubing is shaped using peanut oil heated to 160 degrees C. Sealing these catheters is accomplished simply by heating the tube end with a hot object and pinching it between the fingers. Chronic catheter patency was maintained using Burr's solution (9:1 mixture of glycerine and heparin). No flushing was necessary.

Animals↗

Automated detection of micro-organisms in blood cultures by means of the Malthus Microbiological Growth Analyser.

A prototype Malthus Microbiological Growth Analyser was compared with conventional methods for examining blood cultures in a trial of 651 cultures mostly from patients with haematological malignancy or undergoing haemodialysis or renal transplantation. Of 100 significantly positive cultures, organisms from 82 grew in the conventional aerobic (+ CO2) bottle, 78 in the conventional anaerobic bottle and 71 in the Malthus bottle. The differences were not statistically significant (p greater than 0.05). The Malthus system detected 83.6% of significantly positive cultures earlier than the comparable conventional bottles while 7.3% positive cultures were detected earlier by the conventional system. When use of the Malthus system was restricted to the hours of 09.00 to 17.30 daily 27.3% positive cultures were detected earlier by the Malthus system and 16.4% were detected earlier by the conventional system. One of the organisms which grew in the Malthus bottle, a contaminating Staphylococcus epidermidis, was not detected by the Malthus system. Instability of electrodes resulted in 26.9% false positive cultures with the prototype Malthus system. Contamination rates in both the Malthus and conventional anaerobic bottles were lower than in the aerobic bottles.

Bacteria↗

Comparison of results from two antibiotic susceptibility testing trials that formed part of the United Kingdom national external quality assessment scheme.

A susceptibility testing trial that formed part of the United Kingdom national external quality assessment scheme has been described previously. Results from this first trial showed an association between error rates and particular methods and practices. Changes in methods were recommended where appropriate. A second trial and survey of methods has shown reluctance to change methods and confirmed in most cases that high error rates were associated with the same methods and practices indicated by the first trial. Recommendations on disc content, method of methicillin testing, preparation of inoculum, use of controls and use of lysed blood for sulphonamide testing based on the results from these two trials are restated to encourage laboratories to review their methods critically. A statistical analysis of the results showed significant differences in performance among laboratories, and laboratories whose performance was significantly below the mean were identified. Poor performance was associated with the use of unsatisfactory methods. In view of the critical importance of susceptibility testing in patient care it is intended to use the results of susceptibility testing in the assessment of the performance of laboratories participating in the UK national external quality assessment scheme.

Microbial Sensitivity Tests↗

The effect of digitoxose on feeding behavior.

The effect of digitoxose (DIG) on food intake, gold thioglucose (GTG) lesion formation in the ventromedial hypothalamus (VMH), and VMH glucose oxidation in vitro was investigated in mice. DIG significantly decreased the amount of food ingested during the day compared to controls (p less than 0.01). DIG had no effect on nocturnal feeding. GTG lesion formation in the VMH and VMH glucose oxidation were not altered by DIG treatment. These results suggest that DIG alters daytime feeding behavior by affecting extrahypothalamic or peripheral glucoreceptor sites.

Animals↗

Resistance of the gerbil hypothalamus to gold thioglucose lesion formation.

Mongolian gerbils do not develop ventromedial hypothalamic (VMH) lesions, as seen in mice, when challenged with gold thioglucose (GTG, 800, 1600 and 3600 mg/kg IP). Insulin administration or bilateral adrenalectomy, procedures which sensitize nonresponsive mice to GTG, have no effect in the gerbil given GTG. These findings suggest that the gerbil VMH differs significantly in structure and/or function compared with the mouse by not possessing glucoreceptive cells sensitive to GTG.

Adrenalectomy↗

Systemic phlorizin prevents gold thioglucose necrosis in the ventromedial hypothalamus.

Intraventricular and intrahypothalamic infusions of phlorizin (PHL) are known to cause hyperphagia and to prevent gold thioglucose (GTG) lesion formation in the ventromedial hypothalamus (VMH), respectively. In this study, PHL, administered IP in a large dose (900 mg/kg), completely inhibited GTG necrosis in the VMH. PHL did not cause excessive urinary excretion of GTG. This evidence suggested that systemic PHL must be injected in a high concentration to alter the hypothalamic response to GTG. In vitro measurements of VMH glucose oxidation substantiated this idea. Only at a high concentration of PHL was glucose oxidation significantly depressed in the VMH (p less than 0.001). Small amounts of PHL elevated VMH glucose oxidation (p less than 0.001). Since PHL is an inhibitor of glucose transport, these data support the concept of a hypothalamic glucostatic modulation for the control of food intake.

Animals↗

An antibiotic susceptibility testing trial organised as part of the United Kingdom national external microbiological quality assessment scheme.

Organisms of known susceptibility to antimicrobial drugs were distributed for sensitivity testing to laboratories participating in the United Kingdom National External Microbiological Quality Assessment Scheme. The results obtained were correlated with the methods used. Laboratories differed in their standards of antimicrobial drug sensitivity testing. An association between error rates and particular methods and practices enabled recommendations to be made on disc content, method of methicillin testing, preparation of inoculum, use of controls and use of lysed blood for sulphonamide testing. Some media appeared significantly better than others but because of the many factors involved further information is being sought to clarify this.

Anti-Bacterial Agents↗

Unimpaired quinine metabolism in rats with ventromedial hypothalamic lesions.

Rats with lesions of the ventromedial hypothalamus (VMH) show a greater decrease in food intake than normal rats if fed diets adulterated with quinine. To determine if this hyperresponsivity is due to impaired quinine metabolism, in vitro rates of drug metabolism of female hooded rats with VMH lesions were measured. Determinations were made both under basal conditions and under conditions known to cause induction of drug metabolizing enzymes. VMH rats showed rates of drug metabolism virtually identical to controls under all conditions tested. These data suggest that VMH lesioned rats are not more sensitive to quinine dietary adulteration because of impaired quinine metabolism.

Animals↗