Trematode cataract in fresh-water fish.
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Animals↗
Biomedical subjects
D Easty
Publications and source records attributed to D Easty.
Trematode cataract in fresh water fish.
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Animals↗
Changes in protein expression during melanoma differentiation determined by computer analysis of 2-D gels.
Cytodifferentiation in many melanocytic cells is regulated through the adenylate cyclase-cAMP pathway. To analyse the molecular changes associated with this process we have compared the proteins produced by two closely related cell lines which, though derived from a single cell line, respond very differently to modulation of this signalling pathway. The human melanoma cell line DX3 shows little change in in vitro characteristics following treatment with cAMP elevating agents; in contrast the more malignant DX3 LT5.1 variant, derived from the DX3 parental line, shows pronounced dendrification, decreased proliferation and a reduction in metastatic capacity after similar treatment. The two cell lines were treated with phosphodiesterase inhibitors for 5 days and then processed for two-dimensional gel characterization using an immobilized pH gradient for the IEF dimension. Proteins were detected by silver staining the gels and protein intensities were digitized using a laser densitometer. Two-dimensional gel patterns were edited, matched and a melanoma protein database of 637 spots constructed using PDQUEST software on an Orion 1/05 computer. Eleven proteins were lost and four new proteins were detected in both cell lines following treatment. Twenty-two proteins were present in DX3 LT5.1 after treatment but not in untreated lines or treated DX3. These differentially expressed proteins may be associated with the observed changes in differentiation patterns and metastasis. Our results illustrate the resolving power of this technique and suggest potential applications to the study of cellular differentiation.
Animals↗
Asymmetrical distribution of epithelial membrane antigen on the plasma membranes of human breast cell lines in culture.
A technique for localizing antigens by immunoperoxidase staining on the surfaces of unfixed culture cells is described. The cells can subsequently be studied by light or electron microscopy. These methods have been used to localize epithelial membrane antigen (EMA) on human mammary carcinoma (MCF7) cells in culture. This antigen was expressed on the plasma membrane, being localized on the interface with the culture medium and absent from the other external cell surfaces. This heterogeneous distribution reflects the distribution observed in mammary epithelial cells in vivo.
Antigens, Surface↗
Bankable assets.
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Corneal Transplantation↗