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Biomedical subjects

D E Green

Publications and source records attributed to D E Green.

At least 37 records · Page 2Linked to original sources
PubMed1987

Experimental Argentine hemorrhagic fever in rhesus macaques: virus-specific variations in pathology.

Two isolates of Junin virus (Espindola and Ledesma) inoculated into rhesus macaques produced distinct lesions which were strain-constant and similar to reported human cases of Argentine hemorrhagic fever. The Espindola isolate was associated with hemorrhagia, necrosis of bone marrow, and mild hepatocellular necrosis. Ledesma isolate was associated with pronounced polioencephalomyelitis and autonomic ganglioneuritis, but very mild or absent hepatocellular necrosis, bone marrow necrosis, and hemorrhagia. Deaths of Espindola-infected macaques were usually attributed to hemorrhagia with severe secondary bacterial infections, while in Ledesma-infected macaques, death was associated either with early severe secondary bacterial infections or slowly progressive polioencephalomyelitis. These two Junin virus isolates demonstrated hemorrhagic viscerotropism or neurotropism in macaques, suggesting that the variety of Argentine hemorrhagic fever syndromes in man may be virus-isolate determined.

Animals↗
PubMed1987

Community attitudes to mental illness in New Zealand twenty-two years on.

Three propositions about attitudes to mental illness derived from Nunnally (Popular Conceptions of Mental Health. Holt, Rinehart & Winston, New York, 1961) were examined with the semantic differential technique as it was used originally by Olmsted and Ordway (Final Report to National Institutes of Mental Health, 1963). Attitudes were compared between several studies using the same measures, that ranged over 22 years from 1962 to 1984. The results were remarkably consistent across all studies, indicating that the community had persistently negative attitudes towards the mentally ill and was no more likely today to want to play a major role in the care of the mentally ill than was the case more than 20 years ago. As a consequence it appears that there will need to be substantial and permanent attitude change, of a kind fleetingly observed over the period of the study, before the professional care of the mentally ill in the community may be expected to have maximum impact.

Attitude↗
PubMed1987

Pilomatrix carcinoma.

A pilomatrix carcinoma presented as a large ulcerated mass in the left axilla. Histologically, irregular nests of basaloid matrix cells with cystic centers containing necrotic debris and "shadow cells" were seen. The tumor invaded adjacent structures and was characterized by areas of cellular pleomorphism, crowding of cells, and numerous mitotic figures. An occasional atypical mitosis was noted. A review of 16 cases reported in the world literature is included, with emphasis on epidemiology and treatment.

Axilla↗
PubMed1987

Studies of the coagulation system in arenaviral hemorrhagic fever: experimental infection of strain 13 guinea pigs with Pichinde virus.

Significant coagulation abnormalities were associated with experimental infection of strain 13 guinea pigs with Pichinde virus, an arenavirus related to the virulent human pathogens Junin, Machupo, and Lassa viruses. Infected animals developed decreased activity of multiple coagulation factors, decreased antithrombin III levels, high levels of fibrin-fibrinogen degradation products, impaired platelet function, and thrombocytopenia. Testing for the presence of a coagulation inhibitor revealed a pattern consistent with factor deficiency. Fibrin thrombi were not found at necropsy. The findings of high fibrin-fibrinogen degradation product levels and decreased antithrombin III levels, in association with decreased activity of multiple coagulation factors and thrombocytopenia, suggest that intravascular coagulation is a feature of this experimental infection. The demonstration of abnormal platelet function is also significant, as this could contribute to defective hemostasis despite the moderate thrombocytopenia which usually occurs in arenaviral disease.

Animals↗
PubMed1986

Treatment of junin virus-infected guinea pigs with immune serum: development of late neurological disease.

Guinea pigs infected with Argentine hemorrhagic fever virus (Junin) were treated with pooled, homologous convalescent sera. Use of 15,000 or 5,000 therapeutic units of immune sera prevented all signs of illness when administered within 24 hr of infection. We could also prevent illness and death in infected guinea pigs as late as 6 days after infection if we used more antisera (30,000 therapeutic units/kg). In some treatment groups, surviving animals developed a late neurological syndrome with prominent rear-limb paralysis. Treated animals typically expressed higher viral titers in the brain than in any other organ. There appeared to be no acute exacerbation of disease by antibody administration. Our data suggest that, after replicating peripherally, Junin virus infects the brain where circulating immunoglobulins may not eliminate viable virus. Subsequent replication of virus in the brain may generate a neurological phase of the illness. Histological examination of brains from guinea pigs in treatment groups favoring the neurological phase of illness showed encephalitis, meningitis, and swollen astrocytes, suggestive of neuronal degeneration. There is likely a delicate balance among presence of virus in the brain, the amount of antibody transported into the central nervous system, and the occurrence of this late neurological aspect of experimental Argentine hemorrhagic fever. Further study of this model may elucidate factors relevant in understanding the continuing problem of the late neurological syndrome seen in some human cases of Argentine hemorrhagic fever treated with immune plasma.

Animals↗
PubMed1986

Comparative perceptions of driver ability--a confirmation and expansion.

This study aimed to investigate earlier Swedish and United States research which has shown that drivers tend to rate themselves as more skillful and less risky than the average driver, and to extend the range of driver characteristics on which such ratings are made. A total of 178 male and female drivers rated "me as a driver," "an average driver" and "a very good driver" on the following eight relevant bipolar semantic differential scales: foolish-wise, unpredictable-predictable, unreliable-reliable, inconsiderate-considerate, dangerous-safe, tense-relaxed, worthless-valuable, irresponsible-responsible. The results confirmed expectations that a substantial majority of drivers, up to 80%, would rate themselves above average on a number of important characteristics, but also showed that they rated themselves below "a very good driver." The ratings did not vary significantly across demographic categories. Implications for road safety are briefly discussed.

Adult↗
PubMed1986

Vaginal prosthesis for control of vesicovaginal fistula.

A technique for construction of a vaginal appliance to control urinary incontinence secondary to a vesicovaginal fistula associated with pelvic malignancies and/or radiation therapy is described and illustrated.

Carcinoma, Squamous Cell↗
PubMed1986

Effect of ribavirin and tributylribavirin on argentine hemorrhagic fever (Junin virus) in guinea pigs.

Subcutaneous injections of ribavirin into guinea pigs infected intraperitoneally or intracerebrally with Junin virus significantly increased the mean time to death but did not enhance survival of the animals. We found similar results with tributylribavirin. Virus replication was delayed, but not prevented, in ribavirin-treated infected guinea pigs. The animals usually died with high virus titers in their brains and frequently were paralyzed.

Animals↗
PubMed1985

Hantaan virus infection in suckling mice: virologic and pathologic correlates.

The Hantaan virus suckling mouse model was examined to delineate virologic and histopathologic characteristics of infection at the organ level. Viral antigen and infectious virus were detected in all organs examined, with highest titers achieved in brain, lung, and kidney. A constellation of histologic lesions was identified in brain (diffuse meningoencephalitis with bilaterally symmetrical thalamic necrosis), liver (pericholangiohepatitis), lung (pneumonitis), and spleen (lymphoid hyperplasia); this tetrad is apparently unique to this model system. The chronology of clinical, virologic, serologic, and pathologic findings in Hantaan-infected newborn mice suggests an immune-mediated mechanism in disease pathogenesis.

Animals↗
PubMed1985

Effect of immunosuppression on experimental Argentine hemorrhagic fever in guinea pigs.

Immunosuppression with cyclosporin A or cyclophosphamide had no apparent effect on the disease course of guinea pigs infected with a virulent strain of Junin virus. Immunosuppression of guinea pigs infected with an attenuated strain of Junin virus led to fulminating Argentine hemorrhagic fever. All immunosuppressed infected animals died. Virus distribution patterns in target organs, as determined by plaque assay and fluorescent antibody procedures, were similar to those from non-immunosuppressed animals infected with a virulent strain. Histopathological lesions in immunosuppressed guinea pigs infected with an attenuated strain of virus were similar to those in non-immunosuppressed guinea pigs infected with a virulent strain. Histological changes attributable to the immunosuppressive drug(s) were regularly observed. Immunosuppressed animals infected with attenuated Junin virus and non-immunosuppressed animals infected with virulent virus failed to develop antibody or responded at a minimal level. Virus-specific cytotoxic spleen cell activity, previously shown to be antibody dependent, failed to develop in the same animals. The presence of a competent immune response, probably serum antibody, determined whether Argentine hemorrhagic fever infection of the guinea pig was lethal or whether recovery ensued; no evidence for harmful effects of the immune response was obtained.

Animals↗
PubMed1983

Purification and characterization of the heat-stable calmodulin-binding protein from the matrix of bovine heart mitochondria.

A heat-stable calmodulin binding protein was purified and characterized from the matrix of bovine heart mitochondria. It bound specifically to calmodulin in the presence of calcium, and strongly inhibited the stimulatory activity of calmodulin on phosphodiesterase. The estimated molecular weight of the calmodulin-binding protein was 61,000 dalton determined by SDS-polyacrylamide gel electrophoresis.

Animals↗
PubMed1983

Synthesis, isolation, and characterization of two stereoisomeric ring sulfoxides of thioridazine.

A selective oxidation of thioridazine to give exclusively its ring sulfoxides and a separation of the resulting products as diastereoisomeric pairs of enantiomers (DL, LD and DD, LL) are reported. These pairs were characterized by TLC, high-performance liquid chromatographic, IR, UV, 1H-NMR, 13C-NMR, GC-MS, and elemental analyses, and by reduction to thioridazine by lithium aluminum hydride. Structural data for the separated diastereoisomeric pairs or their nitric acid salts were obtained from NMR and IR studies. Gram quantities of each of the two diastereoisomeric pairs of enantiomers were isolated in better than 99% purity.

Chemical Phenomena↗