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Biomedical subjects

D Drahovsky

Publications and source records attributed to D Drahovsky.

At least 73 records · Page 4Linked to original sources
PubMed1980

C-peptide, testosterone, estrogen, cortisol and zinc in patients with benign hyperplasia of the prostate.

Patients with benign hyperplasia of the prostate (BHP) were studied in relation to aberrant serum c-peptide, testosterone, estrone, cortisol and morning fasting glucose, to analyze the syntropy of diabetes and BHP. The results were compared to patients with bladder cancer without BHP and, after correction of the patients' weights and ages, the control failed to prove any significance. This is consistent with the incidence rate of maturity onset diabetes in prostate adenomas (n = 527, 7.3%) and of a control group (8.5%) of patients, 60--70 years of age. Zinc, another parameter associated with insulin function, is frequently observed to change in BHP and is significantly increased in prostatic tissue which possibly acts as a zinc pool. In this study, however, no effect of prostatectomy on serum zinc was demonstrable even in a long-term control of 9 months, suggesting a sensitive and rapid regulation of this metal independent from the prostate.

Aged↗
PubMed1979

Distribution pattern and enzymic hypermethylation of inverted repetitive DNA sequences in P815 mastocytoma cells.

A specific class of DNA sequences, the inverted repetitive sequences, forms a double-stranded structure within a single linear polynucleotide chain in denatured DNA. The reassociation process is unimolecular and occurs very fast. Quantitative analyses have shown that in mouse P815 cells these sequences comprise about 4% of the nuclear DNA and are interspersed within sequences of other degrees of repetitiveness. After labeling the cells with L-[Me-3H]methionine and [14C]deoxycytidine, relative rates of enzymic DNA methylation were computed on the basis of radioactivities found in pyrimidine residues of the nuclear DNA. The results indicate that in P815 cells, DNA of inverted repetitive sequences is methylated to a level about 50% higher than the normal repetitive DNA sequences and to about 300% higher than the unique and intermediary intermediatry sequences. The biological function of the inverted repetitive sequences, as well as of the role of enzymic methylation of DNA remains unknown.

Animals↗
PubMed1979

[Methods for the detection of antibodies to native (double stranded) DNA (author's transl)].

Three different methods adequate for routine work, i.e. hemagglutination, counterimmunoelectrophoresis and radioimmunoassay, were used in the past in our laboratory to detect antibodies to native (double stranded) DNA. There was a high incidence of false positive or false negative results by counterimmunoelectrophoresis. Hemagglutination reaction was less sensitive in detecting anti-nDNA than was radioimmunoassay. Natural DNA preparations and a synthetic polynucleotide (3H-dAT) were used as antigenic substrates in radioimmunoassay providing well correlating binding values.

Antibodies↗
PubMed1979

Aberrant peptide hormones in patients with urogenital carcinomas.

A series of 229 patients with urogenital carcinomas were investigated for aberrant peptide hormone activities. Serum TSH and prolactin were frequently measured in elevated levels and showed some relation to the stage of disease. Ectopic production of beta-HCG was not observed in any of the cases, thyroid and steroid hormones did not exceed the normal ranges.

Adenocarcinoma↗
PubMed1979

[Distribution and enzymic hypermethylation of inverted DNA repeats in different murine and human cells (author's transl)].

A specific class of DNA sequences, the inverted repetitive sequences, forms a double-stranded structure within a single linear polynucleotide chain in denatured DNA. The reassociation process is unimolecular and occurs very fast. Quantitative analyses have shown that these sequences comprise about 4--5% of the nuclear DNA of various mammalian cells (P815 mouse mastocytoma, Hela, L cells, Raji and Chang cells, and human embryonic hepatocytes) and are interspersed within sequences of other degrees of repetitiveness. After labeling the cells with L-[Methyl-3H]methionine and [14C]deoxycytidine, relative rates of enzymic DNA methylation were computed on the basis of 3H and 14C radioactivities found in pyrimidine residues of the nuclear DNA. The results indicate that DNA of inverted repetitive sequences is methylated to a level about 50% higher than the ordinary repetitive sequences and to about 300% higher than the unique and intermediary sequences. The biological function of the inverted repeats as well as the role of their enzymic hypermethylation is unknown.

Animals↗
PubMed1978

Estrogens in carcinoma of the prostate. Effects on enzymes and polypeptide hormones.

Patients with benign hyperplasia of the prostate and with anaplastic carcinoma have similar activities in their cells in staining for acid phosphatase. After therapy with estrogens the acid phosphatase is significantly inhibited, leucin amino peptidase and succinate dehydrogenase appear to be reactivated in the cells of anaplastic carcinoma. Serum TSH is decreased distinctly, serum levels of LH and prolactin are significantly elevated especially in patients with anaplastic carcinoma of the prostate in comparison to that of patients with treated benign hyperplasia.

Acid Phosphatase↗
PubMed1976

Unmethylated DNA in mouse L cells. I. DNA fibre autoradiography.

Enzymatic methylation of DNA in mouse L cells has been studied using DNA fibre autoradiography to analyse the distribution of 5-methylcytosine in chromosomal DNA. The autoradiographic pattern of DNA labelled in the 5-methylcytosine is in several respects similar to the pattern of DNA replication. Two mean features are apparent: (1) the silver grains appear in well defined sections, and (2) the labelled sections are arranged in tandem along each DNA double helix. After a short pulse of radioactivity in the rate of growth of labeled sections in the pattern of DNA replication and the enzymatic methylation of DNA are identical. Unlike the replication pattern, DNA labeled during the S phase with L-[Me-3H] methionine is not completely labeled. There are distinct, 8-20 mum intervals in the autoradiographic pattern of this DNA. The length of these intervals may correspond to unmethylated sections of chromosomal DNA of about 23 to 58 kilo base pairs. These unmethylated sections of chromosomal DNA represent about 10% of the total genome.

Autoradiography↗