RNA polymerase: potent competitive inhibition by D-ribose-5-triphosphate and other pentose polyphosphates.
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Biomedical subjects
Publications and source records attributed to D Dennis.
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The lactic acid racemase (EC 5.1.2.1) derived from Clostridium butylicum catalyzes the racemization of the alpha-18O label. The proposed alpha-carbonyl intermediate for the enzyme-catalyzed reaction has been previously shown to be trapped as an enzyme-bound oxime in the presence of hydroxylamine. This report demonstrates that the formation of the inactive enzyme-bound oxime, followed by reactivation in the presence of an excess of competing free carbonyl (pyruvic acid) results in a complete loss of the alpha-18O label from an original alpha-18O-labeled lactic acid.
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Examination of the amino acid sequences of human cytochrome c and the alpha-chain variant of human hemoglobin Constant Spring has revealed the possiblity for base-paired hairpin loops in the messenger RNA's for these proteins. A similar analysis of the bacteriophage R17 coat protein suggests an additional unobserved loop in the R17 RNA. If such loops are present in messenger RNA's generally, it would suggest that DNA has more than one stable base-paired conformation.
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Using computer-drafted pressure support gloves during a six-month period, we greatly increased our accuracy of fit. Only eight alterations were required out of 214 gloves manufactured, and none of the alterations was due to computer error. Our time to calculate and lay out patterns decreased by a 4:1 ratio, thus reducing a two-hour time period to one-half hour per glove. The cost of hardware and software was offset by reduced labor costs over a period of approximately eight months. Use of the computer was cost effective, produced error-free patterns, and allowed better control of pattern consistency.
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