Biomedical subjects
D Dean
Publications and source records attributed to D Dean.
Nursing staff must do more for patients dying in hospital.
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Stress test evaluation of patients with poor left ventricular function before and after coronary artery bypass surgery.
Between May 1976 and December 1982 (104 months), 152 patients from a total of 3592 patients who had coronary angiography at the Buffalo Veterans Administration Medical Center had coronary artery disease with left ventricular ejection fractions of 40% or below. Sixty-three patients in this group had coronary bypass surgery. Thirty patients who had graded exercise tests done before and after surgery improved in their peak exercise double product and systolic blood pressure (p value <.05) accompanied by increased work capacity (p value < 0.02). Nineteen patients who had identical exercise protocols also showed increases in exercise time (p value <.05). We believe that coronary artery bypass surgery improves exercise capacity in selected patients with compromised left ventricular function.
Aleurain: a barley thiol protease closely related to mammalian cathepsin H.
We have isolated and sequenced a 1400-base-pair cDNA derived from gibberellic acid-treated aleurone cell mRNA. This sequence contains an open reading frame that would code for a protein of 361 amino acids. The carboxyl-terminal two-thirds of the predicted amino acid sequence is closely related to that of the rat lysosomal thiol protease cathepsin H; the initial 143 amino acids may code for a secretory peptide plus a prosegment. The expression of this aleurone thiol protease mRNA is unusual in that, in aleurone cells, its abundance is regulated by the plant hormones gibberellic acid and abscisic acid, but it is also expressed at high levels in leaf and root tissue. This protease may represent the equivalent of a plant lysosomal thiol protease.
A software package for design and manufacture of prosthetic sockets for transtibial amputees.
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Optimization of neural stimuli based upon a variable threshold potential.
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The story of Bacillus thuringiensis var. israelensis (B.t.i.).
The isolation and the characteristics of Bacillus thuringiensis var. israelensis is reviewed. Included in the review are full descriptions of the nomenclature, mosquito target range of this potent mosquitocidal bacterium, as well as the genetics and biochemistry of the toxins.
Successful treatment of herpes labialis with topical acyclovir.
A double blind, placebo controlled trial of 5% acyclovir cream, applied topically five times a day for five days, was carried out in 49 patients with recurrent herpes labialis. These patients had a total of 74 episodes, 34 of which were treated with the 5% acyclovir cream and 40 with matching placebo. First episodes and all episodes treated with acyclovir cream had significantly shorter times to formation of ulcer or crust and to complete healing (p less than 0.05 for all variables). The duration of all symptoms and proportion of patients developing itching was also reduced by acyclovir cream in first episodes, though the difference was not significant. When the patient started treatment early in the course of a first episode acyclovir cream significantly reduced the percentage of lesions progressing beyond the papular stage (p less than 0.05). Acyclovir cream is well tolerated and effective for the treatment of recurrent herpes labialis.
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
The genes encoding the 52 ribosomal proteins (r-proteins) of Escherichia coli are organized into approximately 19 operons scattered throughout the chromosome. One of these, the spc operon, contains the genes for ten ribosomal proteins: L14, L24, L5, S14, S8, L6, L18, S5, L30 and L15 (rp1N, rp1X, rp1E, rpsN, rpsH, rp1F, rp1R, rpsE, rpmD, and rp1O). We now report the entire 5.9 kb nucleotide sequence of the spc operon. DNA sequence analysis has confirmed the genetic organization and refined the amino acid sequence of the ten r-proteins in this operon. It has also revealed the presence of two open reading frames past the last known gene (L15) of the spc operon. One of these corresponds to a gene (pr1A or secY) which recently has been shown by others to be involved in protein export. In addition, S1 mapping experiments indicate that a significant proportion of transcription initiated from the spc operon continues not only into the two putative genes, but also without termination into the downstream alpha r-protein operon.
Restoration of functional continuity in dystrophic murine muscle after crushing.
The recovery of functional continuity after crush injury was measured by a simple electrophsiological technique in semitendinosus muscles of normal and dystrophic mice of the C57BL/6J(dy2Jdy2J) strain. In contrast to virtually complete restitution in normal muscles, only one-third of fibers regained continuity in dystrophic muscles. The study also confirmed the lower resting membrane potentials of dystrophic fibers and the presence of "functional" denervation in some of them.
Infantile centronuclear myopathy. Evidence suggesting incomplete innervation.
A male case of centronuclear myopathy is reported, with severe weakness at birth and death at 7 weeks. In all the muscles studied the fibres, despite their immature appearances, showed normal histochemical differentiation into type I and type II moieties. In contrast to the extrafusal fibres, the intrafusal fibres seemed to be normal in their development. Although the small centrally-nucleated muscle fibres were equipped with motor end-plates, the EMG revealed profuse fibrillation activity. The conflicting findings are postulated to arise from the presence of inexcitable neuromuscular junctions which nevertheless permitted a neurotrophic influence to be exerted on the muscle fibres.
Application of phase analysis of the Frankenhaeuser-Huxley equations to determine threshold stimulus amplitudes.
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Phase I trial of Staphylococcus aureus Cowan I immunoperfusion.
Staphylococcus aureus Cowan I has shown antitumor activity in in vitro and in animal tumor models. It is hypothesized that this antineoplastic effect results from the interaction of protein A on the cell surface of Cowan I strain S. aureus and immunosuppressive circulating immune complexes. Therefore, we treated five patients with ex vivo plasma immuno-perfusion over killed and fixed S. aureus Cowan I. Toxic effects were marked in all patients and appeared to be related to the plasma volume infused and rate of infusion. Toxic reactions occurred in the cardiovascular, respiratory, and hematopoietic systems. No responses even minimal or transient, were observed in this phase I trial. This toxicity may be reduced if the rate of plasma infusion decreases.
E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site.
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Escherichia coli ribosomal protein S8 feedback regulates part of spc operon.
In Escherichia coli the genes coding for the 52 ribosomal proteins (r-proteins) are organized into a number of transcription units located at various regions on the bacterial genome. The expression of r-protein genes is balanced so that individual r-protein synthesis rates change coordinately in response to changing environmental conditions, and significant amounts of free r-proteins do not exist in the cellular pool. We have suggested a model for the balanced regulation of r-protein gene expression, namely that r-protein synthesis and ribosome assembly are coupled so that r-proteins not incorporated into ribosomes prevent the further translation of r-protein mRNA by a feedback regulatory mechanism. The model was tested in vitro by examining the effect of purified r-proteins on DNA directed r-protein synthesis, and in vivo by examining the effect of overproduction of certain r-proteins on the synthesis rates of other r-proteins. In vitro experiments have revealed that some r-proteins (L1, L4, L10, S4 and S8) can selectively inhibit the synthesis of r-proteins whose genes are in the same operon as their own, and that this specific feedback regulation occurs at the level of translation rather than at the level of transcription of mRNA. Regulatory roles for L1, S4 and L4 have also been established by in vivo experiments. We have studied further the feedback regulatory properties of S8 in vivo and in vitro, and report here that the protein regulates a part of the spc operon.
Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli.
A DNA-directed in vitro protein-synthesizing system was used to demonstrate that r protein S7 has the capacity to inhibit the translation of mRNA for the second and third gene products of the str operon (S7 and EF-G) but not for the first gene product (S12). Translation of mRNA of the last gene product in the operon (EF-Tu) is also probably not inhibited by S7. In addition, we localized the target site for S7 repressor action on the polycistronic str mRNA by examining the repressor activity of S7 in vitro using various template DNAs that contain the gene. The target site was found not to include a promoter-proximal portion of the mRNA for S12. To test for regulatory properties of S7 in vivo, we inserted the S7 gene into a plasmid vector containing the ara regulatory elements such that S7 synthesis was placed under ara control. A specific increase in S7 synthesis caused by stimulation in transcription originating from the arabinose promoter decreased the synthetic rate for EF-G but had no effect on S12 or EF-Tu synthesis.
A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids.
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Pulling staff: problem or solution?
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