Antigen binding to lymphoid cells from unimmunized mice. III. Multiple antigen binding to single B- and T-cells.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
D DeLuca
Publications and source records attributed to D DeLuca.
Antigen binding to lymphoid cells from unimmunized mice. IV. Shedding and reappearance of multiple antigen binding Ig receptors of T- and B-lymphocytes.
Explore the source record for details and available documents.
Animals↗
The specific separation of enzyme antigen-binding cells by substrate affinity chromatography.
Explore the source record for details and available documents.
Animals↗
Specific binding of antigen to lymphocytes. Evidence for lack of unispecificity in antigen-binding cells.
Explore the source record for details and available documents.
Alleles↗
Development of CD4 and CD8 single positive T cells in human thymus organ culture: IL-7 promotes human T cell production by supporting immature T cells.
This paper describes novel model systems to study the development of human T cells. Fragments of neonatal human thymus (HUNT) can be cultured in vitro; the initial majority population of CD4, CD8 double-positive (DP) thymocytes is not maintained in organ culture. These cells are rapidly replaced by populations of CD4 or CD8 single-positive (SP) T cells. In addition, allogeneic thymic chimeras can be established by the addition of human cord blood (HUCB) mononuclear cells as a source of T progenitor cells to the organ cultures. Culture results in the acquisition of a mature SP T cell phenotype by the donor cells similar to that found when HUCB is allowed to develop in xenogeneic murine scid/scid fetal thymus organ culture. The number of immature and mature T cells produced by organ cultures can be differentially increased by the addition of exogenous IL-7, stem cell growth factor, IL-1, or GM-CSF. Anti-IL-7 antibody inhibits T cell production. Taken together, the results suggest that human T cell development occurs in these in vitro systems in a similar manner, regardless of the species origin of the thymic stromal cells in the culture, and that exogenous cytokines can be used to expand subpopulations of developing T cells.
Animals↗