Changes in the adhesive behavior of human osteosarcoma clonal cell lines upon prolonged in vitro culture recovered by cloning.
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Cell Adhesion↗
Biomedical subjects
D De Martelaere
Publications and source records attributed to D De Martelaere.
Stable differences in the adhesive properties of clonal variants derived from a human osteosarcoma.
A human osteosarcoma derived cell line was treated with phorbol-12-myristate-13-acetate and was subsequently cloned extensively. 27 clones were examined for their initial rate of aggregation, their attachment to homo- and heterotypic performed cell layers, and the size of their 24-hour aggregates. Quantitative differences for these three adhesive parameters were observed between the clones. Based on these findings five clones were studied in more detail. The adhesive differences between these clones were stable, reproducible, and dependent on the assay system. This human clonal system will be a valuable tool to study the molecular nature of the adhesive properties of these cells.
Animals↗
Calcium-dependent and calcium-independent aggregation of established and malignant cell lines.
EGTA dissociated cell lines could be distinguished on the basis of their dependence on calcium during initial aggregation. However, the calcium-dependent aggregation could not be abolished by low chymotrypsin/EGTA treatment. These differences were not related to any morphogenetic property of the cells and suggest that different aggregation mechanisms are involved.
Animals↗
Human osteosarcoma derived clonal variants: stable differences in cell surface composition.
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Aminopeptidases↗