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Biomedical subjects

D Cvetković

Publications and source records attributed to D Cvetković.

At least 19 recordsLinked to original sources

Multiobjective satisfaction within an interactive evolutionary design environment.

The paper introduces the concept of an Interactive Evolutionary Design System (IEDS) that supports the engineering designer during the conceptual/preliminary stages of the design process. Requirement during these early stages relates primarily to design search and exploration across a poorly defined space as the designer's knowledge base concerning the problem area develops. Multiobjective satisfaction plays a major role, and objectives are likely to be ill-defined and their relative importance uncertain. Interactive evolutionary search and exploration provides information to the design team that contributes directly to their overall understanding of the problem domain in terms of relevant objectives, constraints, and variable ranges. This paper describes the development of certain elements within an interactive evolutionary conceptual design environment that allows off-line processing of such information leading to a redefinition of the design space. Such redefinition may refer to the inclusion or removal of objectives, changes concerning their relative importance, or the reduction of variable ranges as a better understanding of objective sensitivity is established. The emphasis, therefore, moves from a multiobjective optimization over a preset number of generations to a relatively continuous interactive evolutionary search that results in the optimal definition of both the variable and objective space relating to the design problem at hand. The paper describes those elements of the IEDS relating to such multiobjective information gathering and subsequent design space redefinition.

Algorithms↗

Unusual association of multiple sclerosis and tomaculous neuropathy.

We describe two cases in which multiple sclerosis (MS) occurred in association with tomaculous neuropathy, presenting as chronic, distal sensorimotor polyneuropathy. In Case 1, monoclonal gammopathy of undetermined significance with monoclonal IgG lambda reactive against GM1 ganglioside, was also detected. The diagnosis of tomaculous neuropathy was established after sural nerve biopsy. Teased fibers examination revealed focal 'sausage-like' thickenings of the myelin sheaths in intact fibers and in fibers with segmental demyelination. Electron microscopy showed them to be due, mostly, to multiple windings of redundant myelin and concentric apposition of numerous lamellae, in contact with an intact myelin sheath. These are the first reported cases of tomaculous neuropathy in patients with MS. Whether the combination of the two conditions is purely coincidental or suggests the possible causal relation between MS and tomaculous neuropathy, is not certain.

Adult↗

In vitro cytogenetic analysis of the effects of oxytocin on human peripheral blood lymphocytes.

The purpose of this study was to determine possible genotoxic and cytotoxic (or mitogenic) effects of high concentrations of oxytocin, active component of Syntocinon in cultures of human peripheral blood lymphocytes. Two test systems were used: (1) analysis of numerical and structural chromosome aberrations, and (2) the in vitro sister chromatid exchange (SCE) test. On the basis of the results obtained it can be concluded that oxytocin does not express any genotoxical properties. Furthermore, the mitotic index did not change significantly.

Cells, Cultured↗

Association of Krabbe leukodystrophy and congenital fiber type disproportion.

Hypotonia and weakness developed in a 12-month-old boy whose psychomotor development had previously been normal. The muscle biopsy demonstrated a disparity in the mean diameters of type 1 and type 2 fibers and satisfied major histologic criteria for diagnosis of congenital fiber type disproportion (CFTD). However, deterioration of motor and mental function, which developed subsequently, strongly suggested progressive encephalopathy. Examination of leukocyte cerebral enzymes at 15 months of age revealed a complete lack of galactosylceramide-beta-galactosidase. Selective type 1 fiber atrophy with type 1 fiber predominance has been observed in various conditions, including Krabbe disease. We report an additional case of Krabbe leukodystrophy associated with CFTD. The finding on the molecular level will resolve the dilemma of whether CFTD is a congenital myopathy or whether these patterns of disproportion may result from a number of different processes that interfere with the maturation of the developing motor unit.

Fatal Outcome↗

A genetic study of idiopathic focal dystonias.

The inheritance of focal dystonias was investigated in 43 families containing 43 index cases with torticollis (n = 21), blepharospasm (n = 18) and writer's cramp (n = 4). They generated a potential population of 235 first-degree relatives, and 168 out of 179 living first-degree relatives were examined. Ten relatives with dystonia were identified in ten families. Another two parents from two of the same group of ten families were affected according to the family history. The majority of the secondary cases (six patients, five siblings, and one child) were not aware of any dystonia. The tendency for affected relatives to have the same type of dystonia as index patients was observed only for torticollis. Overall, 23% of index patients had relatives with dystonia. Segregation analysis suggested the presence of an autosomal dominant gene or genes with reduced penetrance underlying focal dystonia.

Adult↗

Different neuronal and glial cell groups in corticomedial amygdala react differently to neonatally administered estrogen.

The percentage of labeled neurons and glial cells in the phylogenetically older corticomedial part of the amygdala was investigated in control and estrogen-treated rats using [3H]thymidine autoradiography. Newborn, three-day-old female and male Wistar rats were treated with a single dose of 1 mg of estrogen and killed at the age of 10 days. The percentage of labeled neuronal and glial cells was determined by stereological methods in the medial, cortical and central nuclei, respectively. In treated male rats, the percentage of labeled neurons and glial cells in these nuclei was significantly greater on the 10th day of life as compared to controls, except for glial cells in the nucleus centralis. In treated female rats, the percentage of labeled neurons in all three nuclei was comparable to controls, while the percentage of labeled glial cells was increased in the nucleus medialis, decreased in the nucleus centralis and unchanged in the nucleus corticalis. Our results indicate clear sex- and region-specific differences in the reactivity of both neurons and glia to neonatally administered estrogen.

Amygdala↗

Study of neurons and glial cells of basolateral amygdala in male and female rats neonatally treated with estrogen.

The influence of estrogen on the neurons and glial cells was examined in the phylogenetically younger, basolateral part of neonatal rat amygdala (nucleus basolateralis and nucleus lateralis posterior). Neonatal rats were treated with a single dose of 1 mg of estrogen on the postnatal day 3, and the [3H]--thymidine ([3H] TdR) incorporation was studied by means of autoradiography. On the postnatal day 10, in treated rats, the percentages of labeled neurons and glial cells significantly increased in nearly all of the nuclei investigated. The only exception was the unchanged percentage of labeled glial cells in the treated female rats (in both of investigated nuclei).

Aging↗

Peritubular Na-K exchange ion pump in maleate-treated frog kidney proximal tubular cells.

1. After perfusion of isolated frog kidneys for 1 hr with 10(-3) or 10(-2) M maleate Ringer, the peritubular membrane potential gradually declined in a dose-dependent manner. 2. The ouabain-like effects of maleate on cell Na and K activities were dose-dependent and smaller than the effects of zero K or 10(-4) M ouabain. Intracellular pH was not altered in the presence of 10(-2) M maleate. 3. The driving force for Na entry into the cell was reduced, respectively, to 81.4 and 58.4% (of control) in the presence of 10(-3) and 10(-2) M maleate. 4. There was no histochemically detectable inhibition of proximal tubule Na-K ATPase activity during 3 hr of perfusion with 10(-2) M maleate.

Animals↗

[The role of Epstein-Barr viruses as etiologic agents in the mononucleosis syndrome].

Mononucleosis syndrome represents a number of symptoms with different etiology and pathogenicity with similar clinical features. The aim of the study was to investigate etiologic structure of mononucleosis syndrome, effects of etiologic factors and age on the severity of clinical features, clinical forms of the disease, complications and the outcome. The investigation was conducted in 46 patients treated at the Clinic who had been diagnosed as having mononucleosis syndrome. EBV-IM was confirmed in 43%, adenoviral IM in 13%, while in 44% of the patients etiology of the disease was not established. In both groups more severe forms were present in patients over 16 years of age, but more frequently in EBV-IM than in other patients with mononucleosis syndrome (40% vs 19.23%). Complications in the form of acute hepatitis were found only in patients with EBV-IM in 20% of the cases, mean age 17 years. We are of the opinion that EBV is a significant etiologic agent in mononucleosis syndrome, that the disease is more severe in older patients who also develop complications. The outcome for all the patients was favorable.

Adolescent↗

The application of hard contact lenses in patients with congenital nystagmus.

From 1973 to 1987, 210 contact lenses were fitted in 112 patients with nystagmus and a refraction anomaly. In 79%, it was possible to correct myopia or a myopic or mixed form of astigmatism. In these cases, the visual acuity improved significantly. The hard contact lenses were well tolerated in all patients, and the intensity of the nystagmus can be reduced through this treatment.

Adolescent↗

[Atherosclerotic changes of the inferior dental artery correlated with those of the carotid bifurcation and the abdominal artery].

50 corpses from adults aged 20 to 75 have been used in order to study the atherosclerotic lesions occurring in typical regions (bifurcation of the common carotid artery and the abdominal aorta) and their relationship to atherosclerotic changes in the inferior alveolar artery. Histological analysis revealed that atherosclerotic alterations of the inferior alveolar artery may appear sometimes earlier than it would be expected on the ground of age. Intima cell proliferation and thickening of elastic elements in the middle layer of the arterial wall, the first signs of atherosclerosis, were found already at the beginning of the third decade of life when the signs of this process in the typical regions were not yet evident. Atherosclerosis affects essentially the functional capacity of the inferior alveolar artery. The development of atherosclerosis in the wall of this artery favours an hypovascularization of the mandible, which must be of certain importance in every operative procedure in oral surgery, especially in those inducing a severe and long traumatism in bone and soft tissues, such as dental implantations.

Adult↗

The genetic variation and covariation among fitness components in Drosophila melanogaster females and males.

The results presented in this study indicate low to moderate level of heritable variation for the following fitness components in Drosophila melanogaster sampled from natural population: early and late fecundity of females, virility of males and longevity of females and males. The most striking exception from this are high additive genetic variances for preadult developmental duration in both sexes. Females exhibit significant negative genetic correlations between early and late components of fitness. In contrast to females, we did not observe any measurable correlations between components of fitness in males. Our data suggest that genetic covariance structure between fitness components differs by sex. We also observed significant negative correlations between virility of males and late components of female life history.

Animals↗