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Biomedical subjects

D Cottalasso

Publications and source records attributed to D Cottalasso.

At least 55 records · Page 3Linked to original sources
PubMed1984

Phospholipids of Golgi subfractions determined with an enzymatic method.

Golgi apparatus subfractions are still under consideration both for morphological, biochemical and functional characterization. In this note, we determined glycerophospholipids and Golgi subfractions from rat liver by a method that requires the complete enzymatic hydrolysis of phospholipids with phospholipase C from CL perfringens. The main advantages of this procedure are that small amounts of protein membranes are required, the precision and the rapidity.

Animals↗
PubMed1982

Analysis of rat liver Golgi apparatus proteins and apolipoproteins in acute ethanol intoxication.

Protein patterns of rat liver secretive (F1, F2) and formative (F3) Golgi fractions and their corresponding VLDLs content have been analyzed by disc-electrophoresis in normal conditions and after acute ethanol intoxication and compared with serum proteins and isolated lipoproteins. The major bands of the normal nascent VLDLs isolated from both formative and secretive Golgi fractions migrate in the same area of serum apo-C and apo-ARP apolipoproteins; in the same density interval of VLDLs another major lipoprotein band, called U, may be separated showing a slower electrophoretic mobility of apo-ARP. After ethanol intoxication apo-C bands seem to be concentrated in secretive and decreased in formative fractions, while apo-ARP and U bands show an overlapping in both Golgi fractions. The possible mechanisms of these behaviours are discussed.

Alcoholic Intoxication↗
PubMed1978

Effects of cicloxilic acid on liver subcellular fractions triglyceride content in acute ethanol intoxication.

The action of cis-2-hydroxy-2-phenyl-cyclohexanecarboxilic acid (cicloxilic acid) on the concentration of triglycerides in the subcellular compartments of the liver was investigated in acutely ethanol-intoxicated rats. Cicloxilic acid is able to significantly reduce the accumulation of neutral fats in the homogenate and in the cytosol and to shorten the steatosis regression time. The triglyceride content in total microsomes results slightly higher in the animals treated with cicloxilic acid than in those treated with ethanol only. The data are discussed in relation to the pathogenesis of ethanol fatty liver and to the possible mechanism of action of cicloxilic acid.

Alcoholic Intoxication↗
PubMed1978

Influence of cicloxilic acid on energy production by hepatocyte mitochondria during acute ethanol intoxication.

Liver mitochondria from acute ethanol intoxicated rats show a highly significant uncoupling of oxidative phosphorylation. cis-2-Hydroxy-2-phenyl-cyclohexanecarboxylic acid (cicloxilic acid) early normalizes the P/O ratio and, therefore, the mitochondrial energy producing mechanisms. The significance of these phenomena and the possible role of cicloxilic acid on mitochondrial energy-production are discussed.

Alcoholic Intoxication↗
PubMed1978

Influence of cicloxilic acid on the intracellular transport of 3H-palmitic acid during acute ethanol fatty liver.

cis-2-Hydroxy-2-phenyl-cyclohexanecarboxilic acid (cicloxilic acid) modifies the rat's hepatocyte intracellular movements of 3H-palmitic acid in the course of fatty liver by acute ethanol intoxication. It counteracts the impairment of radioactive lipid uptake due to ethanol treatment and promotes the early and complete release of the radioisotope inhibited by ethanol. The relevance of these results to the role of changes in the intracellular transport systems in the pathogenesis of ethanol steatosis is discussed. This and previous studies show that cicloxilic acid acts by stimulating the intracellular lipoprotein transport probably preventing by this mechanism the ethanol induced liver injury.

Animals↗