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Biomedical subjects

D Collins

Publications and source records attributed to D Collins.

148 records · Page 9Linked to original sources
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The nurse preceptor: preparation and needs.

This article described a specific experience with a preceptor program. Problems inherent in the preceptor role were presented and appropriate solutions recommended. Each individual preceptor program has factors that are operational, however, many of the concepts and principles of preceptorship are transferable to any setting. By presenting our specific problems and conclusions, other programs may benefit. As professionals, we must continue to disseminate both our successes and difficulties so that professional knowledge can be enhanced.

Education, Nursing, Continuing↗
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General practitioner views of a new specialist Department of Medicine for the Elderly.

The reactions of General Practitioners (GPs) in an area served by a new Department of Medicine for the Elderly (Geriatric Medicine) are described. 89% of GPs thought the new service had brought significant benefits to their patients, The department operated an over 80 admission policy for acute medical admissions. After two years in operation 59% of doctors felt this should be lowered with 4% suggesting it should be raised. Rehabilitation and respite admissions were other features of the new service that had approval ratings of over 70% by the doctors surveyed. It is suggested that a Department of Medicine for the Elderly with a policy of rapid assessment and high turnover is appreciated by GPs. While local circumstances will often dictate policy it is suggested that an age-related admission procedure will minimise difficulty for patient and referring practitioner alike.

Aged↗
PubMedDate not supplied

Snow leopard (Panthera uncia) spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements.

To better understand the biology of snow leopard spermatozoa and to facilitate developing assisted reproduction, a series of studies was conducted to: 1) identify the component(s) of complex culture media responsible for the detrimental effect on sperm survival in vitro, 2) optimize medium for supporting sperm viability, and 3) evaluate sperm capacitation in vitro. Constituents of complex media were added systematically to phosphate-buffered saline (PBS) to isolate the factor(s) influencing snow leopard sperm motility in vitro. Sperm capacitation was also assessed following incubation in PBS with bovine serum albumin (BSA), fetal calf serum (FCS), or heparin. For maintaining sperm motility, there was no benefit (P > or = 0.05) to supplementing PBS with low (5%) or high (20%) concentrations of snow leopard serum (SLS) versus FCS or BSA. Likewise, adding supplemental energy substrates (pyruvate, glucose, lactate, or glutamine) did not enhance or hinder (P > or = 0.05) sperm motility. However, motility rapidly decreased (P < 0.05) with the addition of NaHCO3 to PBS or Ham's F10 nutrient mixture. Surprisingly, Ham's F10 with no buffering component or with both NaHCO3 and N-Z-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) maintained sperm motility at levels similar (P > or = 0.05) to PBS. Although sperm motility in all treatments decreased with time, there was a strong inverse relationship (P < 0.01; r = 0.90) between motility and sample pH at 6 hours. Spermatozoa incubated in PBS containing FCS, BSA, or heparin did not undergo the acrosome reaction when exposed to calcium ionophore. In summary, alkaline pH has a profound detrimental effect on snow leopard sperm motility, and capacitation does not occur under conditions that normally promote this event in other felid species. These results clearly demonstrate a high degree of interspecific variation among felids in fundamental sperm function, and they provide evidence for the necessity of basic research when developing assisted reproduction in little-studied nondomestic species.

Acrosome↗