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Biomedical subjects

D Chorváth

Publications and source records attributed to D Chorváth.

At least 19 recordsLinked to original sources
PubMed1992

[The importance of pulmonary angiography in the surgical treatment of congenital heart defects with a left-right shunt and pulmonary hypertension].

The studied series consisted of 14 patients with ventricular septal defect and pulmonary hypertension aged from 3 months to 15 years. Three examination methods were compared: (1) Invasive hemodynamic examination; (2) Pulmoangiographic examination by means of wedge peripheral pulmoangiography; (3) Histological examination of bioptic samples. Direct correlation was found to exist between mean PA pressure, PAR/m2, TPR/SR and pulmoangiographic records, in which the following parameters were evaluated: (a) length of the narrowing of the peripheral branch of the PA, (b) background opacity, (c) circulation time. The histological findings obtained in the bioptic samples corresponded practically in all cases with the hemodynamic and PAG findings. Only in two patients did the histological picture display a less severe degree of affection than found by PAG and hemodynamic examination. The obtained results suggest that in light of surgical indications, PAG can be considered a suitable and valuable supplementary method in assessing borderline findings of pulmonary hypertension in children with congenital heart defects and left-to-right shunt.

Adolescent↗
PubMed1983

Immunofluorescence and enzyme cytochemical analysis of arterial terminals in the human spleen.

The simultaneous application of immunofluorescent and enzyme cytochemical methods made it possible to analyze the components of the walls of the arterial branches and arterial capillaries, and particularly the spongy arterial terminals in the red pulp of human spleen. The endothelium was identified by alkaline phosphatase demonstration, the basement membranes (BM) by means of antisera to glomerular BM, the media by using the same antisera, and the adventitia by means by antisera to hyaline cartilage, which are presumed to react particularly with adventitial structures. In the spongy arterial terminals visualized by means of anti-hyaline-cartilage antisera, the endothelium and mostly also BM could be detected, yet the enzyme cytochemical reaction to acid phosphatase and alpha-naphthyl acetate esterase and the immunofluorescent reaction to lysozyme yielded negative reactions. The findings suggest that neither macrophages nor other cells containing the given enzymes in detectable form are accumulated in these structures.

Alkaline Phosphatase↗
PubMed1981

A comparative study of the morphology of the systemic anaphylactic reaction (SAR) and the shock reaction induced by antigen-antibody complexes in rabbits.

Differences in the morphological picture of the systemic anaphylactic reaction (SAR), and of the shock reaction induced by antigen-antibody (ag-ab) complexes in rabbits, are described. In rabbits SAR is characterized by acute distension of the lungs, with oedematous swelling in the vicinity of bronchi and veins, intravascular stasis of basophils without degranulation or visible fixation of the inducing antigen, small clusters of blood platelets, leukostasis, minimal decomposition of neutrophil granulocytes and absence of circulating or fixed ag-ab complexes. The shock reaction induced by ag-ab complexes is characterized by acute distension of the lungs without bronchospasm, swelling of the vicinity of bronchi and veins, hyaline thrombi particularly in the pulmonary microcirculation, leukostasis, formation of antigen-antibody complexes and thrombocyte clusters in the microcirculation, and by phagocytosis of these complexes and thrombocytes by various cells.

Anaphylaxis↗
PubMed1980

Use of arylsulphonic acids for histochemical demonstration of cholesterol and its esters.

A histochemical technique is being described using for the demonstration of cholesterol and its esters a reagent composed of sulphuric acid and 2,5-dimethylbenzene sulphonic acid or p-toluene sulphonic acid dissolved in glacial acetic acid. The results of this method have been compared with other methods for cholesterol identification and verified by extraction and chromatographic techniques. The main advantage of the presented procedure is a better preservation of sections, due to the use of smaller amounts of sulphuric acid, permitting thus a more exact histological localization of cholesterol.

Adrenal Cortex↗
PubMed1980

On morphology of anaphylactic reaction elicited by ferritin.

The differences between the systemic anaphylactic reaction (SAR) of guinea-pigs actively sensitized with ferritin and those guinea-pigs who were administered with ferritin-antiferritin complexes, which were of a clinically and macroscopically identical result, are described by means of methods of the morphologic examination. Except some details, SAR of guinea-pigs and rabbits elicited by ferritin possesses a similar morphological picture.

Anaphylaxis↗
PubMed1979

[Morphology of systemic anaphylactic reaction in rabbits to bovine serum albumin and ferritin. I. Manifestations of the reaction after antigen administration: laboratory, macroscopic and optical microscopic findings].

A report is presented on systemic anaphylactic reaction [SAR] of rabbits to bovine serum albumin and ferritin. Active anaphylactic sensibilization with a high yield of sensibilized animals was achieved by administering antigens to rabbits aged a few hours up to 3 months. Tre rabbits showed no presence of precipitins in the circulation, but there were reagins against bovine serum albumin or ferritin. There were various types of SAR activity. Macroscopic findings showed acute emphysema and signs of right heart overload. Apart from acute pulmonary emphysema, histology showed occasional bronchospasm and certain manifestations of RES activation as there was evidence of oedematous infiltration of the peribronchial and perivascular spaces. The above morphological findings cannot as yet be regarded as pathognomonic for SAR.

Anaphylaxis↗
PubMed1978

Nephrotoxic and pneumotoxic antibodies and their acute action on lungs. III. Organ localization of antibodies after experimental administration of lethal doses.

Experimental intravenous administration of large doses of heterologous anti-glomerular and anti-lung antibodies induces acute exsudative haemorrhagic changes in the lungs resulting in death of the experimental animals. The specific localization of these antibodies labelled with radioactive isotopes of iodine and administered using the paired-isotope technique with radioiodinated normal IgG, displayed high values in the lungs, liver and kidneys. Pulmonary changes developing after administration of the same dose of anti-glomerular antibodies into the left heart ventricle are of lesser extent and the animals survive. On this mode of administration, the specific localization of antibodies presents lower values in the lungs, whereas the values in the kidneys and liver are approximately the same as seen after intravenous administration. Antibody localization in the kidneys and liver is facilitated by the morphologic structure of the capillaries, whereas in the lungs a direct transendothelial transfer must be considered, since in this organ capillary endothelium forms a coherent layer. Attention is being drawn to the high specific localization of antibodies in the liver and to the potential effect of these antibodies on the liver.

Animals↗
PubMed1977

Immunohistochemical staining using coupled immunofluorescence and immunocomplex technique.

An immunomicroscopic method combining the use of fluorescent antibodies and enzyme immunocomplex technique is presented. The basic steps of this procedure are: 1. Rabbit antiserum (IgG) to the antigen to be determined, 2. FITC-conjugated antirabbit globulin, 3. Peroxidase + antiperoxidase complex, 4. 3,3-diaminobenzidine + H2O2. The method renders it possible to study simultaneously in the same section antigens and antibodies that are to be localized, and that both by immunofluorescent and immunoenzymatic techniques, it provides means for further elimination of some of the deficiencies of immunofluorescent technique, and in suitable cases it also makes electronoptic examination available.

Animals↗
PubMed1977

[Morphology of ferritin-induced shock in sensitized guinea pigs].

With the aid of morphological methoda, differences have been described between the active ferritin shock and the ferritin-antiferritin shock in guinea-pigs, which clinically appear as identical. The two processes can be differentiated from each other on the light microscopical level, but with particular clarity on the electron microscopical level. In contrast to the active ferritin shock, the guinea pigs in whom the ferritin-antiferritin shock had been produced showed agglomerates of platelets and ferritin-antiferritin complexes either present free in the dirculation or ingested in macrophages or granulocytes.

Animals↗