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Biomedical subjects

D Chia

Publications and source records attributed to D Chia.

79 records · Page 5Linked to original sources
PubMed1975

Persistence of DNA in the circulation of immunized rabbits.

The clearance of deoxyribonucleic acid (DNA) from the circulation was studied in non-immunized New Zealand rabbits and in rabbits immunized with heat-denatured, single-strand DNA (ss-DNA). Animals were injected intravenously with 1-3 mug/kg of 125-I-calf thymus ss-DNA. Initial clearance rates were -0.154 +/- 0.005 per cent (dose/ml/min) in non-immunized rabbits as compared to rates of -0.064 to -0.123 per cent (dose/ml/min) in rabbits immunized with ss-DNA. In immunized rabbits, clearance rates were inversely correlated with relative amounts of antibody, and increased amounts of circulating radiolabel were precipitable with 50 per cent saturated ammonium sulfate, suggesting binding to rabbit immunoglobulin. Thus, the presence of antibody to ss-DNA may delay the usually rapid clearance of ss-DNA from the blood. This observation is similar to that made for a variety of small molecular weight materials, such as insulin, digoxin, and morphine, and is in contrast to that for multivalent protein antigens, such as serum albumin and thyroglobulin. Persistence of DNA in the circulation may be important in the pathogenesis of DNA-anti-DNA-induced immune complex disease.

Animals↗
PubMedDate not supplied

Correlation of disease activity in systemic necrotizing vasculitis with immune complexes.

Ninety-four sera from 23 patients with necrotizing vasculitis of the polyarteritis nodosa (PAN) type were examined for immune complexes by polyethylene glycol (PEG) precipitation. The presence of immune complexes in serum specimens was positively correlated with the presence of active disease in PAN (p less than .001). These complexes predicted active disease 93% of the time and their absence predicted a period of inactivity 54% of the time. The presence of hepatitis B markers, Clq immune complexes, rheumatoid factor, or low complement levels did not correlate with active disease. In 9 patients in whom serial specimens were tested for immune complexes there was a positive association of immune complexes and active PAN.

Adult↗
PubMedDate not supplied

HLA-DR4 in Negro and Mexican rheumatoid arthritis patients.

We tested HLA-DR antigens in 38 Caucasian, 18 Negro, 17 Mexican, and 5 Japanese patients with rheumatoid arthritis. Significantly high HLA-DR4 frequencies were observed in all races; 61% in Caucasians, 39% in Negroes, 77% in Mexicans, and 100% in Japanese. However, no clinical correlation with DR4 was found in these patients.

Arthritis, Rheumatoid↗
PubMedDate not supplied

Relapsing polychondritis associated with ulcerative colitis. Serial determinations of antibodies to cartilage and circulating immune complexes by three assays.

A case of relapsing polychondritis (RP) with severe respiratory tract involvement is reported. The patient's subsequent course included ulcerative colitis and further collapse of nasal cartilage. Antibody titers to cartilage were serially determined by indirect immunofluorescence and levels of circulating immune complexes (CIC) by 3 different techniques. The exacerbations of RP appeared to be reflected by the elevation of anticartilage antibody titers and some elevation in the levels of CIC as detected by the polyethylene glycol method.

Antibodies↗