Massive small bowel resection and abdomino-perineal excision of the rectum.
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Humans↗
Biomedical subjects
D Chatterjee
Publications and source records attributed to D Chatterjee.
Development and characterization of five monoclonal antibodies against neuronal cell surface antigens--evaluation of their use in cell separation by affinity chromatography.
Five monoclonal antibodies directed against rat neuronal cell surface proteins have been isolated and characterized following fusion of mouse SP2/O-Ag-14 myeloma cells and spleen cells from BALB/c mice immunized with neuron-specific membrane proteins. The antibodies displayed cell specificity in binding and elicited precipitation of different polypeptides from total neuronal cell surface proteins. These antibodies are of potential use in the isolation of a neuronal subpopulation by immunoaffinity chromatography. The work represents the application of hybridoma technology for the isolation of specific types of neuronal populations.
Animals↗
Sequence analysis of proteins.
There has been a rapid increase in the number of available protein sequences derived from gene-sequence information. Computer-based sequence analysis of proteins is gaining in importance as an analytical tool. With the help of these analyses such sequences may be characterized and some insights gained into their probable role in the system. The principles involved in computer-based sequence analysis and some of the methods are discussed.
Amino Acid Sequence↗
Distance analysis and sequence properties of functional domains in nucleic acids and proteins.
We present a unified algorithm to analyze distances between short oligomers in large collections of nucleic acids and protein sequences (DISTANP). This extended version of DISTAN methodology not only permits analysis of distances between selected pairs of oligomers, but also allows a user to analyze distances between groups of residues (such as acidic and hydrophobic amino acids). This capacity allows differentiation of sequence properties of known functional domains in nucleic acids and proteins.
Algorithms↗
Sequence of reovirus haemagglutinin predicts a coiled-coil structure.
The use of modern techniques has led to new insights into the molecular mechanisms of viral pathogenesis. Although the infectious process is quite complex, it is clear that one critical stage, the interaction of viral attachment proteins with cell-surface receptors, often has a major role in determining the pattern of infection. The mammalian reoviruses have served as useful models for understanding the molecular basis of viral pathogenesis. The mammalian reovirus haemagglutinin (sigma 1 protein), which is an outer capsid protein, has been shown to be a major factor in determining virus-host cell interactions. To further our understanding of the structure and function of the haemagglutinin, we have cloned a complementary DNA copy of the reovirus type 3 S1 double-stranded RNA gene which encodes the virus haemagglutinin and have sequenced the DNA complementary to the S1 gene. Analysis of the predicted amino-acid sequence of the virus haemagglutinin has allowed us to determine that the amino-terminal portion contains an alpha-helical coiled-coil structure and that the carboxy-terminal portion contains the receptor-interacting domains. Using this information, we propose here a model of how the reovirus haemagglutinin is attached to the virus particle.
Amino Acid Sequence↗
Telomerase activity, Myc and Bcl-2: possible indicators of effective therapy of prostate cancer with 9-nitrocamptothecin.
In this report, we demonstrate the down-regulation of telomerase activity and c-Myc and Bcl-2 expression during 9-nitrocamptothecin (9NC)-induced regression of human DU145 prostate tumors grown as xenografts in immunodeficient mice. These changes were not observed in tumors generated by DU145-derived cells resistant to 9NC. We suggest that telomerase activity, c-Myc and Bcl-2 can collectively serve as molecular diagnostic indicators of the effectiveness of 9NC during treatment of human prostate tumors.
Animals↗
Up-regulation of cyclin B1 and cdc2 expression during 9-nitrocamptothecin-induced regression of DU145 prostate tumor.
BACKGROUND: We investigated changes in the content and subcellular localization of the cell cycle regulators, cyclin B1 and cyclin-dependent kinase cdc2, in human prostate DU145 tumor and cultured cells treated with the anticancer drug 9-nitrocamptothecin (9NC). MATERIALS AND METHODS: Proteins of interest were identified by Western blot methodology using specific antibodies. RESULTS: The cyclin B1 and cdc2 contents were dramatically elevated in biopsies of DU145 tumor regressing upon 9NC-treatment. In vitro, 9NC-induced apoptosis of DU145 cells was associated with up-regulation of expression and nuclear accumulation of cyclin B1 and cdc2. No changes were observed in cyclins A and E and the cyclin-dependent kinase cdk2 in 9NC-treated DU145 tumor and cultured cells. CONCLUSION: 9NC-induced apoptosis in DU145 cells in vivo and in vitro is associated with up-regulation of expression and nuclear localization of cyclin B1 and cdc2.
Antineoplastic Agents↗
Autogenous vein graft to restore ureteric continuity in dogs.
Free, autogenous vein segments were grafted to restore continuity of partially excised ureters in 12 mongrel dogs. Shortening and stricture formation of the vein graft occurred over the following four weeks. There was external adhesion around the graft but no urinary leakage, pooling or fistula. Intubation of the graft maintained patency but shortening occurred. Once the tube was removed, stricture formation and complete obliteration of the lumen of the graft occurred over the following few weeks. Prolonged splinting by biologically inert material may facilitate luminal patency and urothelial lining of the fibrous remnant of the venous graft. Gradual shortening of the graft eventually allows end-to-end anastomosis, without tension, as a second stage procedure.
Animals↗
Renal hemangioendothelioma.
Renal hemangioendothelioma (HE) is an extremely rare malignant tumor. The sixth case is presented and all six analysed. The tumor affects middle-aged persons of either sex with past history of chronic renal disease and very recent onset of renal pain and hematuria. Anemia is common and a renal mass or tenderness is present. Pyelography shows poor function hydronephrosis, filling defect or collecting system distortion. A radical nephrectomy and radiotherapy is indicated and histology establishes the diagnosis. Renal HE appears to take a hurricane course and local recurrence and widespread vascular metastases occur in a matter of days or weeks after traditional cancer therapy. Industrial carcinogenesis is likely while chronic renal disease may predispose.
Female↗
Development of human leukemia U-937 cell sublines resistant to doxorubicin: induction of differentiation and altered sensitivities to topoisomerase-directed drugs.
Cell sublines resistant to doxorubicin (DOX) were developed from the human leukemia cell line, U-937/WT, exposed to stepwise DOX increases. In contrast to U-937/WT cells, the DOX-resistant U-937/RD cells have longer doubling time; are more differentiated along the monocytic lineage as determined by the presence of morphological features and mRNA coding for the monocyte colony-stimulating factor-1 receptor; synthesize the apoptosis-associated Bax protein; are less sensitive to apoptosis-inducing topoisomerase II-directed drugs, apparently because of increased synthesis of P-glycoprotein; and are practically non-tumorigenic when xenografted in nude mice. However, U-937/WT and U-937/RD cells exhibit similar sensitivity to the apoptosis-inducing drug 9-nitrocamptothecin. These findings suggest that several mechanisms are involved in the development of DOX-resistance in U-937 cells, and further, 9-nitrocamptothecin can overcome resistance to DOX. These findings may have clinical implications.
ATP Binding Cassette Transporter, Subfamily B, Mem↗