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D Celińska-Cedro

Publications and source records attributed to D Celińska-Cedro.

15 recordsLinked to original sources
PubMed2001

Primary resistance to clarithromycin in clinical strains of Helicobacter pylori isolated from children in Poland.

Helicobacter pylori resistance to clarithromycin is an important factor in the failure of eradication therapy. The resistance results from point mutations in the 23S rRNA gene of H. pylori. The prevalence of primary resistance of H. pylori to clarithromycin in children and mutations associated with resistance were studied and it was found that 23.5% (23/98) of H. pylori strains isolated in our hospital during 1998-2000 were resistant to clarithromycin. The primary resistance was mainly caused by an A2143G mutation, but the isolates with an A2142G mutation had higher MICs for clarithromycin compared with those with an A2143G mutation: median MIC 256 versus 16 mg/l. Comparison of our data with previous results showed that the prevalence of H. pylori resistance to clarithromycin in children has increased in Poland over the last three years, however the difference was not significant (23.5 vs. 17%, P=0.22).

Adolescent↗
PubMed1999

Genotypes of Helicobacter pylori in Polish population.

Here we have studied the genetic diversity of Helicobacter pylori strains recovered from 64 individual patients, 5 family members and 13 unsuccessfully treated patients. The recovered bacteria were finger-printed by the PCR-RFLP and RAPD methods and virulence associated loci (cagPAI, vacA) were PCR studied. Unique differentiation of every independently isolated strain from not-related persons was possible by RAPD technique. In PCR-RFLP technique several profile groups (7 and 15) for particular endonuclease tested were found. Eleven patients carried strains of the same gene profile (PCR-RFLP) and the same overall genotype (RAPD) before and after therapy. In the family studies, essentially the same strain was found in different relatives in three cases, and different strains were found in the other two cases. Island of cagPAI was present in 79% of all strains tested, half and one-fifth of all strains tested presented, s1am2 and s1m1 alleles of vacA gene, respectively. Independently from identity or diversity of pre- and post-treatment strains and strains recovered from the family members we have been observed identical cagPAI/vacA genotypes. These results suggest that H. pylori infections in Poland can be mixed, although just one strain may often predominate, and that inter-family transmission may be significant even in this high risk society. The genetic feature of virulence-associated loci are similar to those seen elsewhere in Europe, although strains that carry the cagPAI and the potentially more toxigenic alleles of the vacA gene are more common. RAPD technique is proven as most differentiating, however PCR-RFLP allows for easy recognition of mixed infection with two or more different strains. Molecular typing study in case of children therapy may allow reduce rate of relapses by reduction of possible transmission from family source.

Adolescent↗
PubMed1996

PCR for identification and typing of Helicobacter pylori isolated from children.

From 191 children with upper gastroduodenal disease 236 gastric biopsy specimens were taken. H. pylori was detected by use of culture Gram staining histological examination and PCR technique. A segment of DNA coding protein synthesis of 26 kDa or urease A and B gene were used for PCR amplification. PCR technique was also used for determination of the presence of Cag A gene in 72 strains of H. pylori isolated from children. Genetic typing of H. pylori strains by RFLP analysis of PCR amplified urease B gene 933 bp fragment and RAPD were performed. Biopsy specimens taken from children with gastritis were in 52% H. pylori culture and PCR positive, while 18.1% PCR positive only. Similarly, specimens taken from children with duodenal ulcer were in 50% H. pylori culture and PCR positive, while 12.5% PCR positive only. Fifty one (70.8%) from 72 strains of H. pylori were Cag A positive. Molecular typing of the strains isolated during first and follow-up endoscopy allowed the differentiation between reinfection or new infection and coinfection. It was shown that RAPD typing had better discrimination power in comparison to PCR--RFLP method.

Base Sequence↗
PubMed1990

[Esophageal achalasia in children (analysis of five cases)].

Oesophageal achalasia is a disease of middle age and is only exceptionally observed in children. Five patients aged from 6.5 to 14 years were treated for this achalasia. Routine therapeutic method was repeated pneumatic dilatation of the cardia with a Rider-Moeller dilator. In all, 33 such procedures were carried out without complications. Very good results were obtained in 2 cases already after 3 dilatation procedures. The remaining 3 cases required surgical intervention: in 2 of them esophagomyotomy with antireflux operation was done with a very good result in one case and a good result in another case. The child not treated surgically (lack of parental consent) has still most signs of achalasia with body weight below the 3 centile and with recurrent respiratory infections. The follow-up is from 5 to 66 months.

Adolescent↗
PubMed1989

[Barrett esophagus].

An analysis of the clinical picture of Barrett's esophagus in 18 children is presented. This group of patients have been distinguished out of 114 children with chronic gastroesophageal reflux on the base of anatomopathological examinations. Clinical course and efficiency of the diagnostic examinations depend on the degree of esophagitis. They are slightly characteristic in children with minimal lesions. Long-term follow up - necessary due to the risk of cancer - have shown that there is possibility of recovery in cases of minimal advancement and short duration of the disease.

Adolescent↗
PubMed1989

[Fiber-optic endoscopy of the lower gastrointestinal tract in children].

Between 1978 and 1985 565 lower gastrointestinal endoscopies were performed in children. In 221 cases the cause of rectal bleeding was explored, in 227 the suspicion of colitis and in 25 of polyposis was verified. To evaluate the results of treatment (polypectomy, surgical procedures and medical therapy in inflammatory bowel disease) 121 examinations were done. With increasing experience the complete colonoscopy was performed more often. In 1985 coecum and ileum terminale was reached in almost all children in whom it was needed (75% of all examinations). The inflammatory changes were revealed on 230 occasions. In 78 cases colon polyps and in 27 vascular changes were found. The most common cause rectal bleeding were colon polyps (66 cases) and inflammatory changes (554 cases). Less common were vascular changes (10 cases). 32% false negative and 16% false positive results of colonoscopy in diagnosis colitis were obtained when the results of visual findings were compared with histopathological findings. Colonoscopy performed by an experienced colonoscopist rather avoiding general anesthesia is a safe and useful procedure in diagnosing lower gastrointestinal tract diseases in children. The evaluation of colitis should be based on visual findings with biopsy confirmation.

Adolescent↗
PubMedDate not supplied

Campylobacter pylori in upper digestive tract diseases in children.

Results of studies on the frequency of the occurrence of the bacteria, Campylobacter pylori, in the mucous membrane of the gastric antrum are presented in this paper. The study was carried out on 61 children treated for chronic abdominal pain. The diagnosis was established on the basis of flexible endoscopy and histology of antral biopsies. The presence of Campylobacter pylori was determined using the CLO-test. The positive CLO-test was obtained in 87.5% of children with gastritis, in 75% of children with duodenal ulcer and in only 17% of children from control group (p less than 0.001). Four-week therapy with De Nol eradicated Campylobacter pylori in 60% of treated children, and reduced infection in the next 25%. These bacteria seem to play an important role in the pathogenesis of chronic gastritis and peptic ulcer disease.

Adolescent↗