Search PubMed⌕ Search

Biomedical subjects

D Caplan

Publications and source records attributed to D Caplan.

82 records · Page 5Linked to original sources
PubMedDate not supplied

Hemolytic properties of some Aeromonas strains.

Considering the possible correlation between hemolytic and enterotoxigenic properties of Aeromonas strains mentioned in the literature, in the present work we studied the practical value of the hemolysis tests in the diagnosis of Aeromonas strains by using comparatively the hemolysis tube tests (with goat and sheep erythrocytes suspensions) as well as the technique on blood agar in aerobic conditions. There were studied comparatively 230 Aeromonas strains (different species: A. hydrophila, A. sobriae, A. caviae, A. veronii, A. salmonicida) isolated from different sources (meat products, fish of fresh waters, mussels, sea water, pipe water, diarrhoeal disease and animal faeces). The comparison among the used tests for proving the hemolytic activity of Aeromonas strains showed that: 61.89% and 56.51% from the total number of Aeromonas strains were hemolytic in the tube tests with 1% goat and 1% sheep erythrocytes suspensions respectively after 48 hrs followed by 51.72% and 48.25% strains by the 5% goat and 5% sheep blood agar plates respectively in anaerobiosis and after 48 hrs incubation at 37 degrees C. The highest incidence of hemolysin presence was pointed out in motile Aeromonas strains (A. hydrophila--60.11% and A. sobria--56.52%). Our results showing a high frequency of hemolytic activity among Aeromonas strains isolated especially in meat products are suggesting a possible correlation between the pathogenic potential and the hemolytic activity and are pleading for introduction of this test in the diagnosis of Aeromonas species.

Aerobiosis↗
PubMedDate not supplied

[The development of and experimentation with a kit for the identification and rapid biotyping of H. influenzae and H. parainfluenzae].

The paper reports on the drawing up and experimentation of a kit of media for identification in liquid media and biotyping of H. influenza and H. parainfluenza. The kit is made up of 14 components, out of which the following were prepared: basic broth, X V broth, V broth, X broth, red-phenol broth, and XV factors + ribose, xylose and saccharose, urea substrate. Moeller medium with XV, with and without ornithine, covered with paraffin oil after impregnation. In the XV broth and the broth with red phenol the XV factors and saccharose, the bands for indole and H2S were put after impregnation with bacterial culture. On these media, 175 strains of Haemophilus were identified and biotyped. 109 of them were H. influenzae and 66 H. parainfluenzae, when satellitism was used. Identification in liquid media showed that 5.5% of the H. influenza strains were H. parainfluenza and 10.6% of the H. parainfluenza strains were H. influenza. Finally, 110 strains were H. influenzae and 65 H. parainfluenzae. The components of the kit permitting identification and biotyping of H. influenzae and H. parainfluenzae are: basic broth with red phenol, X broth, XV factors supplement, V supplement, mono- and bipotassium phosphate solution, 20% urea solution, 20% saccharose, 20% ornithine, Moeller medium, bands for indole and paraffine oil.

Bacterial Typing Techniques↗