Search PubMed⌕ Search

Biomedical subjects

D C Wilson

Publications and source records attributed to D C Wilson.

At least 91 records · Page 5Linked to original sources
PubMed1986

Calcium entry into cultured mouse astrocytes.

The barium-sensitive component of transmembrane influx of 45Ca into cultured mouse astrocytes was investigated in order to find clues for an involvement of a glial Ca2+ uptake system in Ca2+ movements of the CNS. The Ca2+ turnover was very high amounting to a rate constant of 1.4 min-1. The magnitude of the Ca2+ flux was, however, only about 2% of the K+ flux. It had the same magnitude as the Cl- flux. The Ca2+ influx was not sensitive to the organic Ca2+ channel blockers verapamil and nifedipine; however, cadmium, cobalt and barium blocked the flux in millimolar concentrations. The Ca2+ influx was a linear function of the external Ca2+ concentration up to 1.8 mM. A further increase in external Ca2+ did not increase the magnitude of the influx. Increases in external K+ to the physiological ceiling level of 12 mM (which corresponds to a 15 mV depolarization) and to 54 mM (48 mV depolarization) had no effect on the system. We conclude that the Ca2+ entry system of glial cells is not involved in the decrease of the external Ca2+ concentration during neuronal activity. The barium-evoked spontaneous depolarizations of astrocytes reported in the literature are probably due to the blockade of K+ channels by barium, since 50 microM barium did not affect the Ca2+ flux and increased the total Ca2+:K+ flux ratio from 1:50 to 1:2.5.

Animals↗
PubMed1986

Natural teeth pontics for a cast metal resin-bonded prosthesis.

A technique using natural teeth as pontics for a cast metal resin-bonded prosthesis was described. The patient's periodontally condemned teeth were used for pontics after extraction, endodontic obturation, and preparation for resin bonding to a cast metal framework. The advantages are technique simplicity, esthetics, conservation of natural tooth structure, and reduced laboratory cost.

Acid Etching, Dental↗
PubMed1985

Urinary dihydrodiol metabolites of phenytoin: high-performance liquid chromatographic assay of diastereomeric composition.

Diastereomeric dihydrodiol metabolites of phenytoin, (5S)-5-[(3R,4R)-3,4-dihydroxy-1,5-cyclohexadien-1-yl]-5-phenylh yda ntoin, (S)-DHD, and (5R)-5-[(3R,4R)-3,4-dihydroxy-1,5-cyclohexadien-1-yl]-5-phenylh yda ntoin, (R)-DHD, have been resolved from each other and from urinary constituents with reversed-phase HPLC columns and acetonitrile-water gradients. Recoveries of DHD isomers from urine averaged 99.1% and it was demonstrated that known mixtures of DHD diastereomers added to blank urine were not altered by the assay procedures. The relative diastereomeric content of DHD was determined from integration of the chromatographic peaks. Assay of urine samples from patients on chronic phenytoin therapy and from volunteers indicated that both DHD isomers were present in all samples, and stereoselectivity favored the production of (S)-DHD.

Animals↗
PubMed1982

The viability of pediatric hospices: a case study.

This paper identifies the special characteristics needed by hospices caring for terminally ill children. It is based on a feasibility study conducted in 1979 for a pediatric hospital in New York City. From the analysis of statistical data and confidential interviews with health care professionals in the hospital and in the community, the study concludes that the needs of terminally ill children and their families are not being met currently and that the hospice is as appropriate for children as for adults. Three major differences in emphasis are noted, however. First, support of the family suffering the death of a child is of prime importance, because the grief is devastating and long lasting and because the number of family members affected is usually large. Second, the hospice emphasis on home care is even more imperative for children than for adults as it more significantly benefits both child and family; home care can be facilitated through supplementary care components such as day care. Third, there is a major need for both professional and public education in caring for terminally ill children and their families and in coping with childhood death. The author concludes that further research is needed in all aspects of pediatric terminal care.

Adolescent↗