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Biomedical subjects

D C Shanson

Publications and source records attributed to D C Shanson.

118 records · Page 7Linked to original sources
PubMed1975

A new selective medium for the isolation of Salmonellae other than Salmonella typhi.

A medium consisting of a MacConkey's base with added magnesium, calcium, cobalt, zinc, thiosulphate, novobiocin, iron dextran, Tween 80 and EDTA has been found highly selective for Salmonellaparatyphi B and most of the food poisoning salmonellae that commonly occur in Britain. Nearly all the normal faecal flora is inhibited.

Bacteriological Techniques↗
PubMed1975

An experimental comparison of Thiol broth with Brewer's thioglycollate for anaerobic blood cultures.

In a series of simulated blood culture experiments, small inocula of eight different strains of Bacteroides and five strains of anaerobic cocci were added to Difco Thiol broth and Southern Group Brewer's thioglycollate. Both methods enabled all of the strains to be isolated after one to three days' incubation, with the exception of Bacteroides melaninogenicus, and most strains to survive after one week. B. melaninogenicus grew more quickly in Difco Thiol broth than in Southern Group Brewer's whereas three strains of anaerobic cocci were isolated first from Southern Group Brewer's. Difco Thiol broth appears to be a satisfactory alternative to Southern Group Brewer's for the isolation of non-sporing anaerobes likely to be found in the blood.

Anaerobiosis↗
PubMed1974

An experimental assessment of different anaerobic blood culture methods.

Simulated blood cultures were performed to test the ability of various methods to yield growth from small inocula of different strains of bacteroides and anaerobic cocci. For good early growth and reliable isolation of non-sporing anaerobes a Southern Group Brewers thioglycollate method, without Liquoid, appeared to be the best of the methods tested. A glucose cooked meat method also gave good results. Liquoid had no inhibitory effect on bacteroides. Anaerobic cocci were inhibited by Liquoid in some media, particularly thioglycollate, where one out of six strains tested was completely suppressed.

Anaerobiosis↗
PubMedDate not supplied

Long-term impact of HIV diagnosis on mood and substance use--St Stephen's cohort study.

Twenty HIV positive and 68 HIV negative subjects were assessed by the Hospital Anxiety and Depression Scale and by the Alcohol and Drugs Frequency Schedule immediately prior to notification of their HIV serostatus and 6 months after serodiagnosis. The 2 groups did not differ significantly in levels of anxiety or depression at baseline or follow-up. There were borderline levels of pathological anxiety prior to notification of HIV serostatus in both groups. The drop to normal levels of anxiety which had occurred by follow-up was significant in the HIV positive group. About a third of subjects in both groups were regularly making use of alcohol and/or drugs, both at baseline and follow-up. Mean levels of weekly alcohol intake for both groups ranged from about 20 to 30 units per week. The drugs most commonly used (in any frequency) were nitrates ('poppers') and cannabis.

AIDS Serodiagnosis↗
PubMedDate not supplied

Cryptosporidiosis in HIV-seropositive patients.

The incidence of cryptosporidiosis in our unit has increased over the last 6 years, being diagnosed in approximately 5 per cent of all patients with HIV infection and in 21 per cent of those with AIDS, but a marked seasonal variation occurs. We have studied the course of the infection in 128 patients and identified four clinical patterns of disease: transient (28.7 per cent), chronic (59.7 per cent), fulminant (7.8 per cent) and asymptomatic (3.9 per cent). Transient disease occurred in patients with a wide range of CD4 lymphocyte counts, but was more common in less immunosuppressed patients. Fulminant disease, defined by the passage of more than 2 l of stool/day from the time of presentation, only occurred in patients with a CD4 count less than 50/mm3. This group had lost more than 7 kg in weight at presentation and more commonly had other intercurrent gastrointestinal infections. They survived for a median of only 5 weeks, compared with 20 weeks for those with chronic diarrhoea and 36 weeks for those with transient infection. The survival was unaffected by any treatment other than zidovudine. Cryptosporidiosis in HIV-infected individuals is a heterogeneous disease.

AIDS-Related Opportunistic Infections↗