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Biomedical subjects

D C Burke

Publications and source records attributed to D C Burke.

At least 109 records · Page 6Linked to original sources
PubMed1969

Ribonucleic acid synthesis in chick embryo cells infected with fowl-plague virus.

Increases in ribonucleic acid (RNA) polymerase activity were detected in both the nuclear and ribosomal fractions of chick embryo cells infected with fowl-plague virus. These were observed only in the presence of all four nucleoside triphosphates and were not affected by actinomycin D. The RNA polymerase activity of the ribosomal fraction was shown to be associated with a component of infected cells of sedimentation coefficient approximately 70S. This component also contained infected cell-specific RNA and protein molecules.

Animals↗
PubMed1969

Ribonucleic Acid and protein synthesis in chick embryo cells infected with fowl plague virus.

Fowl plague virus comprised four major protein components and several minor ones, two strains of the virus giving similar results. One of the components was identified as the nucleocapsid protein. Synthesis of the virion proteins could readily be detected in infected cells 3 hr after infection. The two subcellular fractions associated with viral ribonucleic acid (RNA) polymerase activity (nuclei and ribosomal pellet) were associated with the protein of the nucleocapsid and a second virion protein of unidentified function. Measurement of viral RNA and protein synthesis in cells infected with preparations of ultraviolet irradiated virus showed that the capacity to synthesise the RNA and protein species of highest molecular weight was lost most quickly, suggesting that the pieces of viral RNA function independently.

Journal Article↗