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Biomedical subjects

D Bird

Publications and source records attributed to D Bird.

At least 19 recordsLinked to original sources
PubMed2004

Normative data for ultrasound measurement of the calcaneus within different female ethnic groups.

The purpose of this study was to generate normative broadband ultrasound attenuation data for UK resident Indian-Asian, African-Caribbean and Chinese women, aged 20-80 years, using the McCue Cubaclinical II device. Additionally, comparisons were made against available Caucasian data previously collected by the authors. Exclusions were: use of hormone replacement therapy, corticosteroids or thyroxine for more than 6 months; menopause before the age of 45 years; oophrectomy; lactation within the preceding year; rheumatoid arthritis or a previous osteoporotic fracture. 977 women were recruited from various community centres, from a local GP surgery and from university colleges in the London area. Broadband ultrasound attenuation and velocity of sound were determined for the left and right os calces. Repeat measures on each side after re-positioning, to allow for anatomical variation, were averaged. Significance was set at a minimum level of 0.05. There were significant differences in non-dominant and dominant measures in all ethnic groups except African-Caribbean. For comparison purposes the means of the non-dominant measurements were plotted against age using a polynomial model to give the best data fit. No significant difference was found between non-dominant broadband ultrasound attenuation measurements for either Asian or Chinese when compared with the Caucasian sample populations. A significant difference in broadband ultrasound attenuation was found between African-Caribbean and Caucasian, with African-Caribbean between 10% (age group 20-30 years) and 29% (age group 70-80 years) higher. There was no significant difference in body mass index between Caucasian and Chinese groups, but significant differences were found between Caucasian and Asian, and between Caucasian and African-Caribbean groups.

Adult↗
PubMed2003

Anger management training: the effects of a structured programme on the self-reported anger experience of forensic inpatients with learning disability.

Within the current political climate, there is an increasing burden on mental health professionals to achieve accuracy in risk assessment and prediction. The accurate assessment and treatment of anger can make a valuable contribution towards alleviating this burden as part of a comprehensive treatment package. This study describes an anger management training programme provided to a group of three forensic inpatients with learning disability. An ABA single case study design was used, with anger levels assessed at weekly intervals before, during and after the programme. The results suggested that anger management training is useful with this client group, with established tools, such as the Novaco Anger Scale, the Spielberger State-Trait Anger Expression Inventory and the Modified Overt Aggression Scale, allowing the impact of the programme on anger levels to be evaluated. There is an indication that maintenance treatment is required to prevent anger levels increasing to pretest levels following treatment.

Adult↗
PubMed2002

Fibre-optic two-photon scanning fluorescence microscopy.

Two geometries of a novel two-photon fluorescence microscope incorporating single-mode fibre optics for the delivery of ultrashort-pulsed illumination to a remote sample are characterized. First, a 785 nm single-mode optical fibre is implemented in a scanning microscope, which demonstrates that an improvement in axial resolution is achieved due to the non-linear response of the fibre to intense ultrashort-pulsed light. Second, a 785 nm single-mode optical fibre coupler is adapted, in which case spectral broadening and blue shifting of the ultrashort-pulsed laser beam caused by the non-linear response of the fibre to ultrashort-pulsed illumination are experimentally characterized. An investigation into the impact of temporal broadening of the ultrashort-pulsed beam on the systems is also considered. The coupling efficiency of both geometries for various illumination wavelengths is also presented. The introduction of the fibre coupler to the system has significant advantages, including an improved optical sectioning effect and a reduction in the number of bulk optical components resulting in a low-cost, compact instrument. Sets of three-dimensional images of fluorescent polymer microspheres and biological material confirm these features.

Eukaryota↗
PubMed2001

Impact of early onset bipolar disorder on family functioning: adolescents' perceptions of family dynamics, communication, and problems.

OBJECTIVE: This research investigated the impact of adolescent onset bipolar illness on perceived family functioning in stabilized bipolar I (B) and unipolar (U) probands, and normal controls (C). METHOD: Sample N=119: 44 bipolar 1(17 M, 27 F), 30 unipolar (9 M, 21 F), and 45 controls (19 M, 26 F). Mean ages: 19.9, 18.5 and 18.2 years, respectively. INSTRUMENTS: Family Adaptability and Cohesion Scale (FACES II), Parent-Adolescent Communication Scales (PACS), Social Adjustment Inventory for Children and Adolescents (SAICA). RESULTS: There were no significant group or sex differences between controls and mood disordered youth--assessed intermorbidly--in ratings of relationship with either parent. Bipolars acknowledged significantly more minor conflicts with parents than either unipolars or controls. Ratings by mood disordered subjects were significantly less positive in terms of shared activities and communication with siblings. Mood disordered youth and controls were not differentiated on the basis of family adaptability, and all family cohesion scores were within population norms. No significant group differences were observed in communication with parents. LIMITATIONS: This self-report study was conducted intermorbidly, does not include objective measures of family functioning, nor does it assess the effect of psychiatric illness in other family members on family functioning. CONCLUSIONS: Assessed intermorbidly, bipolar adolescents' perceptions of family dynamics do not seem to diverge significantly from controls. Further research is needed to investigate the impact of adolescent bipolar illness on family life during acute phases of the illness, as well as the effect on family functioning of psychiatric disorders in other family members.

Adolescent↗
PubMed2001

Overlapping plant signal transduction pathways induced by a parasitic nematode and a rhizobial endosymbiont.

Root-knot nematodes and rhizobia establish interactions with roots characterized by the de novo induction of host structures, termed giant cells and nodules, respectively. Two transcription regulators, PHAN and KNOX, required for the establishment of meristems were previously shown to be expressed in tomato giant cells. We isolated the orthologues of PHAN and KNOX (Mt-phan and Mt-knox-1) from the model legume Medicago truncatula, and established the spatial distribution of their expression in situ. We confirmed that Mt-phan and Mt-knox-1 are expressed in lateral root initials and in nematode-induced giant cells and showed that they are expressed in nodules induced by Sinorhizobium meliloti. Expression of both genes becomes spatially restricted as the nodules develop. We further examined nematode feeding sites for the expression of two genes involved in nodule formation, ccs52 (encodes a mitotic inhibitor) and ENOD40 (encodes an early, nodulation mitogen), and found transcripts of both genes to be present in and around giant cells induced in Medicago. Collectively, these results reveal common elements of host responses to mutualistic and parasitic plant endosymbionts and imply that overlapping regulatory pathways lead to giant cells and nodules. We discuss these pathways in the context of phytohormones and parallels between beneficial symbiosis and disease.

Amino Acid Sequence↗
PubMed2001

High-quality, cost-effective strategy for detection of autoantibodies to extractable nuclear antigens.

We evaluated methods for the detection of autoantibodies to extractable nuclear antigens (ENAs) to determine the strategy that yielded the most cost effective and clinically meaningful result. We prospectively compared counterimmunoelectrophoresis (CIEP) with and without serum prediffusion (SPD) and found that SPD significantly improved the quality of precipitation lines. This resulted in a decreased requirement for repeat testing and, consequently, was associated with a significant decrease in reagent costs and specimen turnaround time. We also retrospectively compared reactivity by CIEP, CIEP plus SPD, enzyme-linked immunosorbent assay (ELISA), and line immunoassay (LIA) of 52 serum samples that were previously determined to be positive for ENAs, and we correlated the results with clinical diagnoses. There was significant agreement among CIEP, CIEP plus SPD, ELISA, and LIA for the detection of anti-SS-A, anti-SS-B and anti-RNP. In general, CIEP, CIEP plus SPD, and LIA correlated better with the clinical diagnoses than ELISA, even though ELISA detected anti-ENAs more often than the other methods. CIEP plus SPD is therefore the most cost effective method for the identification of clinically meaningful ENAs. Based on our experience, we now screen for ENAs by CIEP, and positive samples are then typed by CIEP plus SPD. Samples that are difficult to interpret are then further assessed by an alternative method.

Antigens, Nuclear↗
PubMed2001

Bilateral variation in calcaneal broadband ultrasound attenuation: part I.

A total of 1412 women of various ethnic origins ages 20-80 yr were tested for bilateral calcaneal broad-band ultrasound attenuation (BUA) with the McCue Cubaclinical ultrasound bone densitometer. A significant difference was found between nondominant and dominant measures (p < 0.0001). The magnitude of calcaneal BUA was not related to dominance. The difference was of a significant degree to have resulted in differing predictions of fracture risk depending on the side used in assessment. Forty-eight subjects (3%) would have been considered at risk of osteoporotic fracture based on the nondominant measure, whereas only 21 subjects (1.5%) would have been considered to be at risk based on the dominant measure. In concurrence with other studies, we conclude that it is essential to carry out bilateral measurements of the os calcis to avoid misleading implications for prediction of fracture risk.

Adult↗
PubMed2001

Bilateral variation in calcaneal broadband ultrasound attenuation. Part II: as measured by three bone densitometers employing ultrasound or X-ray.

In previous study, we found a bilateral difference in broadband ultrasound attenuation (BUA) of the calcaneus, using the McCue Cubaclinical II device. The purpose of the present study was to determine whether a calcaneal bilateral difference (greater than that that would be expected from the coefficient of variation) was also found by other instruments and their technologies. Twenty-four subjects (ages 22-81) were each scanned by the same operator using three devices: the Cubaclinical (ultrasound [US], BUA), the Lunar Achilles plus (US, BUA and stiffness index), and the Pixi (dual X-ray absorptiometry, bone mineral density). To allow for the different variables used by the three devices, the following computation was applied to the data for comparison purposes (left - right) / (right). Analysis of variance showed no significant difference. We conclude that the three devices do not differ in their ability to detect a proportional difference between right and left calcanei. In an additional investigation, using the Cubaclinical, 23 subjects were scanned. The direction of the US wave between emitter and receiver transducers was reversed to determine the effect, if any, on bilateral BUA readings. In this case, the direction of the US wave was not influential.

Absorptiometry, Photon↗
PubMed2001

Characterization of northern pintail (Anas acuta) ejaculate and the effect of sperm preservation on fertility.

Northern pintail duck semen and sperm traits were characterized, and the fertility of cold-stored spermatozoa was investigated using artificial insemination. Excellent quality ejaculates containing high proportions of motile spermatozoa were collected from drakes within 20 s by a massage technique. Semen was collected in Beltsville poultry semen extender, pooled and cold-stored (4 degrees C) for 0, 24, 48 or 72 h. Hens were inseminated with 100 microl twice a week, and eggs were assessed for fertilization and hatch success. Fertilization success was similar (P > 0.05) for semen cold-stored for 0 (51.6%), 24 (51.5%), 48 (41.1%) and 72 h (22.3%; P > 0.05). Similar (P > 0.05) percentages of fertilized eggs hatched to live offspring (73.1, 71.4, 87.0 and 80.0%, respectively). Fresh semen was also equilibrated with 1 or 4% dimethylsulphoxide or glycerol, and cryopreserved at the following rates: (1) approximately 60 degrees C min(-1) (in liquid nitrogen [LN(2)] vapour) for 10 min; (2) 1 degrees C min(-1) to -20 degrees C, LN(2) vapour for 10 min; and (3) 1 degrees C min(-1) to -35 degrees C, all followed by immersion in LN(2). After thawing for 30 s at 37 degrees C or 20 min at 4 degrees C, sperm motility and viability were assessed. The highest numbers of motile spermatozoa were recovered after slow-fast freezing (2) and thawing at 0 degrees C (P < 0.05), but survival was inadequate to allow artificial insemination. Nonetheless, cold storage provides an effective means of short-term storage with no loss of fertility in this waterfowl species.

Animals↗
PubMed2000

Getting it right? An exploration of issues relating to the biological sciences in nurse education and nursing practice.

Concerns have been expressed that bioscience education is not meeting the needs of nursing students in the UK. This paper explores the situation further by comparing student perceptions with those of experienced practitioners (Part One of the study) and also evaluates the confidence of staff nurses in explaining the rationale for care applied to a common but specific disorder (influenza; Part Two). Questionnaires were used. Responses were elicited from DipHE Nursing (Project 2000) adult/child branch students (n=153) from two universities and from adult/child care staff from their local clinical placements (n=171 in Part One of study; n=266 in Part Two). The questions asked followed two themes: (1) confidence in understanding biological science and (2) issues of teaching and learning. Most questions utilized a rating scale from 1 to 10; this scale provides no central value and a vertical line was drawn to encourage respondents to identify which half of the scale their responses came under (basically, a negative or positive viewpoint). The significance of the data distribution either side of the midline was analysed statistically (simple sign test), as were any differences in distribution between the groups (chi-square test). Median values were also determined. The data do not provide support for views that the biosciences are being significantly diminished by modern curricula, but also show no evidence for a great improvement in the bioscience knowledge base in recent years. What was surprising was the lack of confidence expressed by staff nurses: one illuminating finding was the lack of confidence in articulating their knowledge to patients and, even less so, to other health professionals. The paper reiterates a need for a national teaching and learning strategy for pre- and postregistration education in the biosciences. In particular, a means to raise the level of understanding of staff nurses must be identified urgently so that the mentoring of students in these subjects is improved.

Adult↗
PubMed2000

Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.

High-dose estrogen both stimulates new medullary bone formation and suppresses hematopoiesis in mouse long bones. To determine whether the latter response is a direct consequence of the former, we compared the time course of estrogen's effects on osteogenesis and hematopoietic bone marrow. Flow cytometry was employed to measure hematopoietic subpopulations in bone marrow from femurs of female mice killed at different times after commencing 0.5 mg estradiol/wk to each animal. Estrogen markedly reduced the number of leucocytes (CD11a positive), which had already diminished by 75% after 4 days and had virtually disappeared by 18 days. Specific populations showed a similar pattern of decline after estrogen, including B lymphocytes, monocytes, and endothelial cells. In contrast, the osteogenic precursor population showed a marked increase after estrogen treatment, as assessed by assaying alkaline phosphatase-positive colony-forming units (fibroblastic) ex vivo. However, this rise did not reach significance until 8 days after estrogen administration, suggesting that it follows rather than precedes estrogen's effects on hematopoiesis. We conclude that estrogen does not suppress hematopoiesis in mouse long bones as a direct consequence of its effects on osteogenesis.

Animals↗
PubMed1999

Electrical injuries.

Never underestimate the severity of the injury. History taking must be meticulous and thorough. Accurately assess percent of skin loss--fluid resuscitate as necessary. Monitor for the presence of cardiac arrhythmias. Observe for the development of compartment syndrome. Observe volume and colour of passed urine. Seek expert opinion.

Electric Injuries↗
PubMed1999

Managing epistaxis in A&E.

The emergency nurse should never underestimate the volume of blood that can be lost from epistaxis. It is important to be alert for respiratory obstruction or distress and vigilant for shock like symptoms. Examine for underlying disorders and seek advice from ENT specialists, monitoring vital signs. Finally, a patient with a nasal pack in situ must be admitted to hospital.

Emergency Nursing↗
PubMed1999

Transferring the thermally injured.

The emergency nurse should seek expert opinion if in doubt. The priority is to protect the airway--intubate if unsure. Administer oxygen and insert at least two 16fg cannula in adults and two 20fg cannula in children. Record all fluids administered including the type, volume and rate of infusion. Monitor and record clinical observations (see Fig. 3). Keep the burns wounds covered with cling-film. Measure urine output and ensure the catheter circuit is patent and ensure the patient is pain free. Keep the patient warm and relatives informed. Finally, inform the burns unit of departure. Details provided by A&E staff may have considerable impact upon the subsequent management of the thermally injured patient.

Burn Units↗
PubMed1998

Premorbid functioning in adolescent onset bipolar I disorder: a preliminary report from an ongoing study.

BACKGROUND: This study reports on premorbid academic and peer functioning and psychiatric illness in a rigorously diagnosed sample (N = 28) of adolescent onset bipolar I patients. METHODS: Premorbid functioning was assessed by parental report and review of the Ontario School Record (OSR). Premorbid psychiatric diagnoses were assigned on the basis of all information gathered. RESULTS: Overall, findings suggest that this cohort demonstrates good to excellent peer and academic functioning prior to illness onset. Rates of premorbid psychiatric illnesses were similar to that described in epidemiologic samples. CONCLUSIONS: Results are discussed in relation to current understanding of early onset bipolar illness and directions for future research.

Achievement↗