Biomedical subjects
D Bernoco
Publications and source records attributed to D Bernoco.
HLA antigens in recurrent stromal herpes simplex virus keratitis.
HLA-A, HLA-B, and HLA-C antigens were typed on 48 patients with recurrent herpes stromal keratitis. The HLA-Aw30 antigen occurred three times more frequently in patients with herpes stromal keratitis than in those who lack the Aw30 antigen. When the data for the probability of the HLA-Aw30 were corrected for the number of variables studied, the corrected P value was not significant. A previous report of an increased frequency of HLA-B5 with recurrent herpes keratitis was not confirmed by our study. No significant associations with the HLA-C antigens were noted. HLA-DR antigen typing of 25 herpes stromal keratitis patients indicated that the HLA-DRw3 antigen appeared to have an increased frequency in herpes stromal keratitis. However, the small sample size studied precluded interpretation of the increased phenotypic frequency and the possible association of HLA-DRw3 and herpes stromal keratitis needs further study.
Lymphocytotoxic definition of combined ABH and Lewis antigens and their transfer from sera to lymphocytes.
Analysis of lymphocytotoxic reactions with peripheral blood lymphocytes from 74 donors, typed for ABO, secretor, and Lewis phenotypes, identified clusters of reactions distinguishing six antigens resulting from the interactions of Lewis, secretor, and ABO systems: Lea, Leb, ALed, BLed, ALeb, and BLeb. In these experiments, Led on O lymphocytes and Lec were not detected as expected from the experience of other authors with A antigen, B and Leb were detected only on the lymphocytes of ABH secretors, demonstrating that all the ABH antigens of lymphocytes are controlled by the secretor system as are the ABH antigens in external secretions. The ABH and Lewis antigens identified on lymphocytes could be transferred in vitro to lymphocytes, cultured for 2 to 7 days at 37 degrees C in the serum of donors of selected ABO, Lewis, and secretor phenotypes, confirming that ABH and Lewis antigens are not synthesized by lymphocytes but are acquired from circulation as are the Lewis antigens on erythrocytes. As expected, the HLA antigens of lymphocytes were not modified after culture.
Analysis of alloantisera against bovine lymphocytes. Joint report of the 1st International Bovine Lymphocyte Antigen (BoLA) workshop.
The results and agreements of the 1 international BoLA workshop, held in Edinburgh, Scotland in August 1978, are reported. Most of these concern the results from a comparison test of 249 alloantisera to bovine lymphocytes, the antisera being contributed by 9 laboratories. These sera were compared directly in Edinburgh on a panel of lymphocytes from 130 cattle of 21 breeds. In the microlymphocytotoxicity test used 75% of the sera reacted. Sixty eight of these sera were grouped into clusters according to their reaction patterns against the lymphocyte panel. Eleven of these clusters were clearly defined and were given workshop BoLA designations. In addition 22 sera were assigned to subgroups of the agreed clusters. There was no evidence that the method of production of the sera had any effect on their specificity. Although genetic data was not available, the phenotypes of the test panel of lymphocytes are consistent with the clusters detecting antigens controlled by multiple alleles at a single autosomal locus. It was agreed to name the genetic region where this putative locus is located BoLA (bovine lymphocyte antigen).
ABH and Lewis antigens on lymphocytes: screening of pregnant women's sera with the B-cell cytotoxicity test.
Five phenotypes related to the ABH-Lewis substances were defined by the B-cell lymphocytotoxic test. These five antigens are built up in a stepwise fashion by the action of the glycosyl transferases specified by the Le, H, and the A, B genes onto the same ABO percursor molecules. The comparison of their chemical structures with the cluster analysis obtained by cytotoxicity suggests that antibodies reacting with large antigenic structures (pentasaccharides) have a more restricted specificity than antibodies reacting only with smaller antigenic determinants (trisaccharides).
Two independent HLA-B-locus specificities expressed on a single haplotype.
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Twins with two different fathers identified by HLA.
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Microdroplet testing for HLA-A, -B, -C, and -D antigens. The Phillip Levine Award Lecture.
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International cell exchange.
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Five subtypic specificities of the Bw22 complex in four racial groups.
Five subtypic specificities of Bw22 were defined using 38 informative local and Seventh Histocompatibility Workshop sera: Bw54, 22.2, J2, Bw42, and a new Bw22 associated antigen Te90. The previously defined specificity, 22.1, associated with Cw3, could not be distinguished from Bw54 in this study. Marked restriction in the distribution of four Bw22 specificities (Bw54 excepted) in white, Mexican, black, and Oriental populations was described and provided supporting evidence for the identification of these antigens.
Evidence for a second B-cell locus separate from the DR locus.
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Studies of HLA-A, -B, -C, and -D antigens on monocytes.
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Autoantibody against B lymphocytes.
The sera of 20 out of 99 (20%) normal males and females were shown to have antibodies that kill their own B lymphocytes. The sera were specifically cytotoxic to B lymphocytes and not T lymphocytes. Allogeneic B lymphocytes were also killed by these autocytotoxins. Maximum killing of B lymphocytes occurred when the incubation temperature in serum was 5 degrees C and complement incubation temperature was 20 degrees C. Under these cold conditions, some sera reacted up to a dilution of 1/16. These results suggest that positive B-lymphocyte crossmatches found in kidney transplantation do not detect alloantibodies. Since the B-cell autoantibodies tend to appear at the time of immunisation, their role as autoregulators of the antibody response is an intriguing possibility.
Structural heterogeneity of C2 Complement protein and its genetic variants in man: a new polymorphism of the HLA region.
A zymogram method, following thin-layer isoelectric focusing in a polyacrylamide gel, allows resolution of the lytic activity of serum C2 complement protein in a spectrum of molecular forms. This spectrum is characteristic in each of the species studied (man, rhesus monkey, guinea pig, and hamster). Moreover, two different alternative patterns are observed in man: each of the six major lytic bands characteristic of the most common pattern (herein designated C2(1) is duplicated in the least common pattern (C2(2-1), with an additional band displaced cathodally by not more than 0.04 pH unit. Distribution of phenotypes C2(1) and C2(2-1) in a Caucasion population is in agreement with the hypothesis that they are controlled by two alleles, C2(1) and C2(2), with frequencies 0.96 and 0.04 +/- 0.01. Segregation studies show that the two alleles are codominant and identify a locus in the HLA region. No recombinants with HLA-B were detected among 27 informative meioses, generating a cumulative lod score of 6.321 at equals 0. These findings suggest that the individuals with the C2-deficient trait might be interpreted as homozygotes for a third and rarest amorph C2 degrees of the same locus.
B cell antigens of the HLA system: a simple serotyping technique based on non-cytotoxic anti-beta2-microglobulin reagents.
A modification of the NIH cytotoxicity test for recognizing B cell (D-associated) antigens and antibodies, when sera also contain anti-HLA(ABC) activity is described. The method is based on the observation that anti-beta2-microglobulin reagents are able to block lympholysis only when due to HLA(ABC) antigens.
Genetic analysis and biological properties of products of the third SD (AJ) locus of the HL-A region.
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Fate of HL-A antigens and antibodies at the lymphocyte surface.
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Cell-mediated lympholysis in vitro with normal lymphocytes as target: specificity and cross-reactivity of the test.
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