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Biomedical subjects

D Benjamin

Publications and source records attributed to D Benjamin.

At least 217 records · Page 12Linked to original sources
PubMed1981

Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.

Seventy-six consecutive patients presenting to a genital herpes simplex virus (HSV) clinic were enrolled in a study comparing viral isolation (VI), indirect immunoperoxidase (indirect IP), and direct immunofluorescence (direct FA) techniques for the detection of HSV antigen. Of the 76 patients, 61 (80%) demonstrated HSV by VI, compared with 66% by indirect IP and 55% by direct FA (P less than 0.05). Genital lesions from nine patients demonstrated HSV antigen by direct FA or indirect IP but were VI negative; eight of nine patients had subsequent episodes of genital HSV confirmed by VI. During the vesicular-pustular stage of the disease, VI was positive in 90%, indirect IP was positive in 76%, and direct FA was positive in 71% of the lesions, whereas with ulcerative lesions, VI was positive in 72%, indirect IP was positive in 55%, and direct FA was positive in 38%. These commercially available rapid viral diagnostic techniques are specific and useful, if adequate specimens are obtained from early genital lesions.

Antigens, Viral↗
PubMed1980

Oxygen affinity of hemoglobin in polycythemia of undetermined etiology.

High oxygen affinity hemoglobinopathy, as an eventual cause for polycythemia of undetermined etiology, was investigated in 40 unclassifiable polycythemia subjects. The determination of 2,3-diphosphoglycerate and P50 in the polycythemic and in the control groups has not demonstrated any sigificant difference between them. The routine search of this condition in similar patients does not seem to be warranted.

2,3-Diphosphoglycerate↗
PubMed1980

Hemochromatosis in a renal transplant recipient.

A 47 year old kidney transplant recipient who died from liver failure caused by hemochromatosis, is described. The diagnosis was established by post mortem examination. The question whether these findings are an extreme form of the common pathological changes seen in the liver in other transplant recipients, or were related to infectious hepatitis or were due to the use of immunosuppressive therapy, remains unanswered.

Female↗
PubMed1980

Viral hepatitis with extreme hyperbilirubinemia, massive hemolysis and encephalopathy in a patient with a new G6PD variant.

Extreme hyperbilirubinemia, massive hemolysis and hepatic encephalopathy were reported in a G6PD-deficient patient with viral hepatitis. Investigation of the G6PD properties revealed that this patient's enzyme represented a hitherto undescribed variant (Bnei Brak), characterized by heat stability and a unique triphasic pH-related enzyme activity curve. It is possible that the marked hemolytic process in this patient was enhanced by a further decrease in the activity of the already deficient enzyme during acidosis.

Adolescent↗
PubMed1979

Use and functional properties of peripheral blood lymphocytes in mice.

Peripheral blood lymphocytes (PBL) were isolated and examined for their ability to respond in vitro to H-2 and H-Y antigens and also to hapten conjugated syngeneic cells. In addition, these lymphocytes were tested as antigen and target cells in in vitro mixed lymphocyte reaction and in chromium release cell mediated lympholysis respectively, as well as serving as targets for the H-2 typing of chimeric mice. Two methods were used to isolate the lymphocytes: a density gradient separation and a double water lysis technique. PBL, prepared by either method compared favourably with splenic lymphocytes in all aspects of anti-H-2 cytotoxicity but could not be used as responder for anti-H-Y cytotoxicity. In addition a method has been developed using PBL as targets for H-2 typing of both allophenic and irradiation chimeric mice. The small numbers of cells required for each of these procedures allows for the preselection of suitable mice and/or multiple experimental determinations on individual mice.

Animals↗
PubMed1979

Experimental nasopharyngitis and pneumonia caused by Chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant.

Three infant male baboons were inoculated with a strain of CHLAMYDIA TRACHOMATIS ISOLATED FROM A HUMAN INFANT WITH PNEUMONITIS. One baboon, inoculated by intratracheal, nasopharyngeal, and oropharyngeal seeding, had rales, radiographic evidence of pneumonia, persistent nasopharyngeal C. trachomatis infection, and a four-fold rise in titer of antibody. At sacrifice 24 days after inoculation, nasopharynx, trachea, airways, and lung yielded C. trachomatis, and epithelial inclusions were seen by light and immunofluorescent microscopy. Histopathologic changes noted were nearly identical to those in a lung biopsy specimen from a human infant and pneumonitis and nasopharyngeal C. trachomatis. The second baboon was inoculated by tracheal seeding and maintained nasopharyngeal C. trachomatis until killed 30 days later. Autopsy revealed nasopharyngitis and patchy mild pneumonitis. The third baboon was inoculated by nasopharyngeal seeding and maintained nasopharyngeal C. trachomatis for 49 days. Both of the latter baboons seroconverted. Infant baboons appear to be useful animal models for C. trachomatis nasopharyngitis and pneumonia.

Animals↗
PubMed1979

Prolonged busulfan-induced remissions in chronic myeloid leukemia.

Two prolonged remissions were achieved in a patient with chronic myeloid leukemia by two short courses of busulfan treatment. The first remission lasted for 7 years; the second one lasts already 14 years. In the interval periods no treatment was administered.

Busulfan↗
PubMed1979

Migration inhibition factor activity in sera of patients with chronic lymphatic leukemia.

Migration inhibition factor (MIF) activity, expressed as a migration index, was studied in the sera of 48 chronic lymphatic leukemia (CLL) patients and 48 healthy controls. MIF activity was detected in the sera of 50% of the CLL patients. The medical condition of patients in advanced clinical stages (III and IV) and with detectable MIF activity was more stable (after 18-mo follow-up) than was that of the patients in advanced stages but without detectable MIF activity. No relationship was found between the clinical stage of the disease, absolute lymphocyte count, and MIF activity.

Adult↗