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D Barzilai

Publications and source records attributed to D Barzilai.

At least 91 records · Page 5Linked to original sources
PubMed1979

Ultrastructural observations on the capillaries of human thyroid tumours.

Ultrastructure of the capillaries of malignant and benign thyroid tumours has been examined. The material consisted of biopsies from six cases of thyroid papillary carcinoma, one case of follicular (foetal type) adenoma and six cases of nodular adenomatous goitre. In the group of nodular adenomatous goitre and in the follicular adenoma, the capillary wall was made up of fenestrated endothelium similar to that of capillaries of normal human thyroid. The fenestrae occupied a large area of the endothelial wall. Micro- and macropinocytotic vesicles were frequent in the endothelial cytoplasm. In the thyroid carcinomas the papillary structures always contained numerous capillaries with fenestrated endothelium. The microfollicular area and the solid tumoral areas of the papillary carcinoma showed occasional capillaries with fenestrated endothelium, but many capillaries were lined with continuous endothelium. The capillaries in all the specimens were surrounded externally by a continuous basement membrane which was frequently bilaminate or multilaminate. This study indicates that capillaries with fenestrated endothelium are characteristic of thyroid tumours which arise from follicular cells.

Adenoma↗
PubMed1979

Glucocorticoid receptor cleavage by leupeptin-sensitive enzymes in rat kidney cytosol.

The proteolytic origin of small fragments of both the glucocorticoid and mineralocorticoid receptors in rat kidney cytosol was inferred from the effects of leupeptin, a bacterial tripeptide that inhibits many proteases [Sherman, M.R. et al., (1978). Federation Proc. 37:167--173]. In the present study, the smallest fragment of the glucocorticoid receptor containing the steroid-binding site, the mero-receptor, was characterized with respect to the Stokes radius (RS = 23 +/- 3 A) and the isoelectric point (pI = 5.9 at 4 degrees). Chromatography of cytosol labeled with [3H]triamcinolone acetonide on Sephadex LH-20 (Pharmacia) in aqueous buffer resolved the steroid-receptor complex from the unmodified free steroid and from steroid metabolites and contaminants. This technique facilitated analyses of the leupeptin-stabilized receptor form by isoelectric focusing (pU = 5.9 at 4 degrees) and centrifugation in glycerol gradients (s20,w = 9--11 S in 50mM KCl). When this large complex in fresh cytosol was analyzed on Agarose (Bio-Rad) at a high flow rate, it had RS congruent to 60 A in 50 mM KCl and RS congruent to 30 A in 400 mM KCl. These analytical studies with leupeptin indicate the need for inexpensive, irreversible inhibitors of proteolytic enzymes for the purification of intact receptors, holo-receptors, from kidney and other tissues. Specific proteases can then be applied to dissect the holo-receptor into the globular mero-receptor, proximal to the steroid-binding site, and the asymmetric region(s), distal segment(s), that may be involved in the nuclear interactions.

Animals↗
PubMed1979

Characterization of the specific binding of prolactin to binding sites in the seminal vesicle of the rat.

The binding of ovine prolactin to the seminal vesicles of the rat has been characterized and found to be a saturable process, dependent upon time, temperature, protein concentration of the seminal vesicle and divalent ions. Its specificity was similar to that reported for prolactin binding to other organ preparations. Time and temperature studies of the specific binding revealed that equilibrium was reached after 16 h at 5 degrees C or 4 h at 19 degrees C. Nonspecific binding was also dependent on time and temperature. This parameter has been reported to comprise up to 70% of the total binding to various organ-binding sites, but it fell to below 20% after 48 h at 19 degrees C, thus demonstrating the high degree of specificity required of target organ receptors. From degradation studies it was evident that no damage occurred to the free hormone during incubation for up to 70 h at 5 degrees C or 16 h at 19 degrees C. However, there seems to be a difference in the susceptibility of bound and free ovine prolactin to damage during incubation: after 40 h at 19 degrees C the hormone in the supernatant fraction had lost 85% of its binding ability, whereas a high level of specific binding was evident in the pellet. A Scatchard plot of competitive binding studies revealed two classes of binding sites, of which the high-affinity, low-capacity site was similar to that reported previously and consistent with a physiological receptor for prolactin in the seminal vesicle of the rat.

Animals↗
PubMed1979

Ultrastructural pathology with normal light microscopy of liver in Wilson's disease. A case report.

Light microscopy findings in early Wilson's disease (hepatolenticular degeneration) may be normal even when special cytochemical stains are used. We present a case of Wilson's disease in which light microscopy was negative, while electron microscopy showed the characteristic changes in the hepatocytes. Since low serum ceruloplasmin levels and high urinary copper excretion are not by themselves definite proof of Wilson's disease, and since copper content of the liver is not universally measured, electron microscopy examination of liver tissue appears to be a worthwhile additional tool for the early diagnosis of Wilson's disease.

Adult↗
PubMed1978

Chlorthalidone-induced syndrome of inappropriate secretion of antidiuretic hormone.

A 60-year-old woman who had been instructed to increase her water intake because of nephrolithiasis developed the syndrome of inappropriate secretion of antidiuretic hormone when treated with chlorthalidone for mild hypertension. Serum osmolality was 235 mOsm/kg with concomitant urine osmolality of 490 mOsm/kg. When serum sodium decreased to 110 mEq/liter, plasma antidiuretic hormone (ADH) was elevated at 30 pg/ml. The syndrome resolved when chlorthalidone was discontinued together with fluid intake restriction. Plasma ADH returned to normal (less than 0.5 pg/ml) after three days of treatment. The favorable outcome in this patient is attributed to early recognition of the syndrome, which might occur even with nonthiazide diuretics such as chlorthalidone.

Chlorthalidone↗
PubMed1978

Cholestatic jaundice caused by D-penicillamine.

D-penicillamine is not generally considered to cause hepatic damage. Cholestatic jaundice developed in a patient with rheumatoid arthritis 4 weeks after penicillamine was added to his regimen, and he died in acute renal failure. The probability that penicillamine caused the cholestasis is discussed.

Arthritis, Rheumatoid↗
PubMed1978

Hepatic injury due to pyridinol carbamate.

Two cases of severe hepatic parenchymal injury are presented. One case can be attributed to pyridinol carbamate with certainty, and this drug was probably the cause of hepatic injury in the second case. Pyridinol carbamate should be added to the list of drugs which are known to cause parenchymal liver damage.

Aged↗
PubMed1978

Antidiuretic hormone secretion and urine flow in aged catheterized patients.

Repeated measurements of urine flow, every 10 min, and plasma ADH levels, every 20 min, were done in six aged, supine catheterized patients for seven hours. Orthogonal spectral analysis revealed 80 to 130 ultradian rhythms in urinary osmolality and in Na+ and K+ concentrations, but not in the flow of urine that fluctuated rapidly. Plasma ADH levels were constant, ranging from 0.5 pg/ml to 1.5 pg/ml, without evidence for pulsatile episodic secretion.

Aged↗
PubMed1977

[Liver damage caused by adelphan].

Hepatic damage due to Adelphan or to one of its two components (Rauwolfia alkaloids [Reserpin] and 1,4-dihydrazinophthalazine [Nepresol]) has not thus far been described. A patient is presented who repeatedly reacted to Adelphan with fever, chills and hepatic damage. Adelphan should therefore be added to the list of drugs which may cause hepatic damage.

Aged↗