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Biomedical subjects

D Bartels

Publications and source records attributed to D Bartels.

At least 19 recordsLinked to original sources
PubMed1992

A desiccation-related Elip-like gene from the resurrection plant Craterostigma plantagineum is regulated by light and ABA.

The resurrection plant Craterostigma plantagineum tolerates an extreme loss of cellular water. Therefore this plant is being studied as model system to analyse desiccation tolerance at the molecular level. Upon dehydration, new transcripts are abundantly expressed in different tissues of the plant. One such desiccation-related nuclear gene (dsp-22 for desiccation stress protein) encodes a mature 21 kDa protein which accumulates in the chloroplasts. Sequence analysis indicates that dsp-22 is closely related to early light inducible genes (Elip) of higher plants and to a carotene biosynthesis related gene (cbr) isolated from the green alga Dunaliella bardawil. In contrast to other desiccation-related genes, light is an essential positive factor regulating the expression of dsp-22: ABA-mediated gene activation leads to the accumulation of the transcript only in the presence of light. During the desiccation process, light acts at the transcriptional and post-transcriptional levels. The implications of these different controls and the possible role of the dsp-22 protein in the desiccation/rehydration process are discussed.

Abscisic Acid↗
PubMed1992

Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco.

Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3'-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.

Biological Transport↗
PubMed1991

An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein.

In most higher plants a period of desiccation is the terminal event in embryogenesis. Excised barley embryos acquire desiccation tolerance at a precise developmental stage and cDNA clones have been isolated which are temporally linked with desiccation tolerance. One such clone (pG22-69) with a putative gene product of 34 kd displays high structural homology to mammalian genes encoding an NADPH dependent aldose reductase involved in the synthesis of sorbitol. This first aldose reductase gene of plants is expressed constitutively during embryo maturation and is modulated by the plant hormones abscisic acid (ABA) and gibberellic acid (GA). Immunohistochemistry showed that the protein is preferentially expressed in tissues formed at early stages in embryogenesis. Measurements of enzymatic activity indicate that pG22-69 encodes an active aldose reductase. The finding of this reductase activity and the cloning of the corresponding gene supports the existence of a metabolic pathway in plants playing a role in the synthesis of osmolytes like sorbitol. The significance of this work is that genes of related structure and functions are being used in diverse organisms to fulfil stress related biological requirements.

Abscisic Acid↗
PubMed1989

Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes.

The isolation and characterisation by DNA sequencing of a low molecular weight (LMW) glutenin gene from wheat is described. The deduced protein contains a signal peptide, a central repetitive region rich in proline and glutamine and N and C terminal non-repetitive domains, similar to other prolamins. A detailed comparison of the C terminal domain of 20 prolamin genes enabled us to divide them into 4 families. The LMW glutenin family is distinct from the alpha, beta- and gamma-gliadin families of wheat and is closest to the B hordein genes of barley. This and other comparisons were also used to assess the pattern of genetic variation among prolamin sequences and to provide a molecular basis for the interpretation of prolamin size polymorphism. The 5' flanking fragment of the isolated gene was previously shown to direct endosperm-specific expression of a reporter gene in transgenic tobacco. Evidence is provided that the isolated gene is also active in wheat and its transcription initiation site was determined. Features of the gene which may be relevant to its activity are discussed.

Amino Acid Sequence↗
PubMed1988

Regulating IVF.

Explore the source record for details and available documents.

Australia↗
PubMed1988

[Reconstruction of unilateral iliac vein occlusion].

From 1 July 1971 through 6 June 1987, 46 of 10,644 reconstructive vascular interventions were Palma-operations. The morphological operability was ascertained by ascending phlebography and the functional indication by phlebodynamometry. The autologous contralateral saphenous vein was used in 19 patients as a transplant, whereas 27 patients received a spiral-wrap polytetrafluoraethylene (PTFE) prosthesis. All patients were given a temporary av-fistula. Followup was possible in 15 of the 19 autologous operations and in 25 of the 27 PTFE operations. Ten vein transplants and 21 PTFE transplants were still patent.

Adult↗
PubMed1987

Diltiazem and propranolol in mild to moderate essential hypertension as monotherapy or with hydrochlorothiazide.

We compared the safety and efficacy of diltiazem and propranolol, and examined demographic factors influencing responses to these agents. One hundred ninety-six patients with supine diastolic blood pressures of 95 to 114 mm Hg were treated with propranolol (80 to 240 mg twice a day) or a sustained-release preparation of diltiazem (60 to 180 mg twice a day) in a double-blind, randomized, parallel group protocol for 6 months. Hydrochlorothiazide was added for patients not achieving the treatment goal. Both agents produced nearly identical and highly significant (p less than 0.001) reductions in supine blood pressure. There were no significant differences at the end of the optional combination therapy phase, although additional reduction with hydrochlorothiazide was slightly greater in the propranolol group. Blood pressure responses in relation to age, gender, race, and smoking history showed that diltiazem produced greater changes in older subjects and women, whereas propranolol was less effective in blacks. However, these differences were not critical.

Adult↗
PubMed1987

Clinical nurse specialists: an institutional process for determining priorities.

This article describes a four-step process designed by a midwest teaching hospital to identify priorities for the appointment and use of clinical nurse specialists. In an era of cost containment where the outcomes and effectiveness of staff positions are continually questioned, such a process enhances the objectivity and quality of administrative decision making.

Humans↗
PubMed1986

DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat.

Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. The results suggest that the hybridizing fragments contain single gene copies and permit the identification of the subunit encoded by each gene. Restriction-fragment variation associated with previously established allelic differences between varieties was observed. Also, there is a clear relationship between the electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it. These results are discussed with reference to the evolution of the HMW glutenin gene family and the uses of restriction-fragment variation in plant breeding and genetics.

Alleles↗
PubMed1986

[Telemetric labor monitoring using the fetal monitor BMT 941-1].

Recommendation of a telemetric system for the direct fetal monitoring using the monitor BMT 914-1 (Zwönitz). The free movement of parturients during labor is more comfortable and involves an increasing uterine activity, a labor progress and a decrease of oxytocic infusion rate.

Electrocardiography↗
PubMed1985

Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit.

A high molecular weight glutenin gene in hexaploid wheat has been isolated by cloning in bacteriophage lambda and characterized. The gene corresponds to polypeptide 12 encoded by chromosome 1D in the variety "Chinese Spring". The coding sequence predicted contains seven cysteine residues six of which flank a central repetitive region comprising more than 70% of the polypeptide. These findings are related to the role of high molecular weight subunits in the viscoelastic theory of gluten structure.

Amino Acid Sequence↗
PubMed1985

[10 years' experience with the in situ bypass].

From October 1, 1974 to March 31, 1984 467 femoropopliteal and femorotibial in situ vein bypasses were performed. Valvular incompetence is produced by the use of modified Hall strippers. The intraoperative angiography is mandatory to localize the tributaries and to detect pathological findings of the vein and technical errors. Overall cumulative patency rates were 84, 76, 71, and 67%, at 2, 5, 8 and 10 years. Cumulative patency rates for 117 tibial bypasses separately were 72, 68, and 68% at 2, 5 and 8 years.

Angiography↗