[Acute pulmonary edema in cerebral malaria (author's transl)].
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Humans↗
Biomedical subjects
D Baron
Publications and source records attributed to D Baron.
Helix pomatia agglutinin (HpA) affinity chromatography: the isolation of pure B and T cell populations and their use for the routine HLA-DR (Ia) serology.
The anti-A1 agglutinin (HpA) of the albumin gland of the snail Helix pomatia binds specifically to neuraminidase-treated T lymphocytes. After its immobilization on Sepharose-6MB an affinity matrix is obtained which is able to separate T and B lymphocytes. In 40 independent experiments enriched T and B cell populations were isolated either by this HpA affinity technique or by E-rosettes incubated with sheep red blood cells for 2 h or 15 h. The results showed that with the HpA affinity matrix within 3 h a highly enriched B cell population is isolated with a purity of 80% and a yield of 65%. Since the separation is done within a short period of time and under gentle conditions the percentage of dead cells is not more than 5%, which is a requirement when the cells are to be used for immunofluorescence studies of surface markers and serological determinations involving microscopical scoring. Typing for HLA-A, B, C antigens and for HLA-DR alloantigens can be done on the same day by cytotoxicity assay. The method also provides a clear-cut distinction between the HLA-A, B, C antibodies and the HLA-DR antibodies.
Agglutinins↗
[Prolonged neuromuscular blockade during a D. penicillamine-induced myasthenia (author's transl)].
The authors report a D. Penicillamine-induced myasthenia gravis in a patient with rheumatoid arthritis. The disorder was revealed by a postoperative respiratory depression which could be attributed to the aggravation of the neuromuscular blockade by the muscle relaxants. D. Penicillamine-induced myasthenia gravis is now well known. The onset of such an accident after anaesthesia of patient treated with D. Penicillamine is a potential risk. Management of these patients in anaesthesia must be similar to the management of patients with classic myasthenia gravis.
Adult↗
The comparison between normal and Epstein-Barr virus-transformed homozygous typing cells and their use for MLC, PLT and serological detection of human Ia-type alloantigens.
Normal human lymphocytes isolated from freshly drawn blood were repeatedly stimulated (up to five times) in mixed lymphocyte cultures (MLC) with five different Epstein-Barr virus (EBV)-transformed homozygous typing cells (HTC-LCL). Each time, the same WDW-specificity was used for the restimulation. The first and the second stimulations resulted in an 2.4- to 3.1-fold increase of the original responder cell number. But after the third, fourth and fifth stimulation decreasing amounts of specifically primed lymphocytes were recovered. Primed lymphocyte typing (PLT) showed significant differences in the intensity of the stimulation depending on whether homozygous typing cells (HTC) or HTC-LCL were used as stimulators. Except for one cell type, the PLT-response was significantly stronger with HTC-LCL than with HTC. The time needed to reach the maximal PLT-response got shorter and shorter the more often the cells were reprimed by the same stimulator cell. Further differences between HTC and HTC-LCL were observed in the complement-dependent cytotoxicity assay: HTC-LCL were more sensitive towards rabbit complement and they showed an enhanced binding of anti-Ia-alloantibodies but HTC reacted more specifically with the antibodies.
B-Lymphocytes↗
Oral pindolol in acute evolving myocardial infarction.
Pindolol was administered orally (5 mg 8th-hourly) to 30 patients 5--19 (mean = 9.0 hrs) hours after onset of acute myocardial infarction. There were no serious side effects during the acute phase of infarction, only one hospital death and two late deaths at follow-up 2--82 (mean = 37 wks) weeks after hospital discharge. In contrast to healthy volunteers, patients with acute infarction had unpredictable absorption of pindolol from the gastrointestinal tract; this was attributable in part to concurrent administration of narcotic analgesics.
Administration, Oral↗
Steroid-cyclophosphamide pretreatment of kidney allograft donors. A control study.
34 cadaveric donor grafts were randomized in a blind study of the effect of pretreatment of 5 g each of methylprednisolone and cyclophosphamide on kidney graft outcome. There was no difference in overall survival or functioning after 3, 6 or 12 months between grafts from pretreated (33 kidneys) or control (29 kidneys) cadaveric donors. In addition, this pretreatment protocol did not modify the recipient immune response against B-lymphocyte alloantigens which developed in unsuccessful transplants. Our data, thus, neither confirm the high rate of kidney graft survival attributed to cadaveric donor treatment nor the supposition that treatment is effective in suppressing recipient antidonor B-lymphocyte antibodies.
Adolescent↗
Insulin-dependent diabetes and HLA.
The study of a hundred and fifteen unrelated insulin-dependent diabetes and eight families with at least two insulin-dependent diabetes members made it possible to confirm the higher frequency of HLA-B8 and B18 (p less than 0.001) among patients, producing a RR of 2.24 and 2.47 respectively. The increased B15 frequency did not achieve statistical significance. B18 whose gametic association (delta = 0.0438) was significant only in diabetic patients was often related to Aw19-2 (Aw30 + Aw31). The B8/B18 genotype gave a relative risk (RR = 4.98) which was significantly higher than that of B8, B18 and B15 heterozygotes (1.50, 1.24 and 1.39 respectively). Pairs of diabetic siblings were more frequently HLA identical than would be expected by chance, and distribution of the pairs of affected sibs into the three categories, identical, semi-identical and different, was closer to the recessive model than to the dominant one. The fact that the B8/B18 individuals had a RR slightly higher than the B8 and B18 homozygotes and distinctly higher than the heterozygotes for only one of these genes, favours the hypothesis of two dominant genes, giving the appearance of recessivity. The gene associated with B18 in Southern Europe seems to play the same part as that of the gene associated with B15 in Northern Europe.
Adolescent↗
Synergistical effects of ficoll and phytohemagglutinin on human lymphocytes.
The influence of increasing concentration of the highly polymerized dectran Ficoll on cultured peripheral human blood lymphocytes stimulated or not stimulated by PHA was studied. The incorporation of 14C-thymidine into acid-insoluble material of unstimulated lymphocytes has not been influenced in the presence of increasing concentrations of Ficoll either pretreated with chelating resin or not pretreated. Ficoll not pretreated with chelating resin potentiates the PHA-induced stimulation by the factor 4.3 at 0.1 mg Ficoll/ml culture medium, and by the factor 3.2 at 1.0 mg/ml when PHA stimulated HPBL were used. Ficoll after pretreatment with chelating resin does not influence the DNA-synthesis at 0.1 mg Ficoll/ml medium, but causes a drop of the incorporation of 14C-thymidine by the factor 2.0 at 1.0 mg/ml. Using 10.0 mg per ml, both Ficoll preparation cause a decrease of the DNA-synthesis by the factor 2.5--3.0--probably a cytotoxic effect. The results obtained with emission spectrographic analysis and conductivity measurements show, that Ficoll after treatment with chelating resin contains about 10 times more ions (Mg, Ca, Na, Si) and has a 3.2-fold higher conductivity then Ficoll without pretreatment. It is possible that phenomena like electrochemical changes on the surface of the lymphocytes and osmotic alterations in the culture medium are responsible for these effects.
Cells, Cultured↗
Partial purification and properties of the Epstein-Barr virus-associated nuclear antigen.
The Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) was purified 85-fold from a nuclear pellet derived from an EBV-transformed B lyphoblastoid cell line by a five-step procedure consisting of preparation of extract, heating at 80 degrees C in phosphate buffer, ammonium sulfate precipitation, preparative ultracentrifugation, and affinity chromatography on double-stranded DNA-cellulose. The purified complement fixing antigen specifically blocked the anticomplement immunofluorescence assay for EBNA. Several properties indicate a close association of EBNA with chromatin, viz. 1) precipitation of antigenic activity by phosphate buffer and subsequent thermal fractionation; 2) partial sensitivity of antigenic activity to DNase (but not to RNase) and restoration of activity by addition of calf thymus DNA; and 3) specific binding of EBNA to double-stranded DNA-cellulose. Other properties of EBNA, including its unusual heat stability, are described.
Antibodies, Antinuclear↗
Left ventricular function in tetanus: influence of therapy.
Nine patients hospitalized for tetanus were studied under the same protocol which included two haemodynamic studies with a preload trial (PLT). The first being made during therapy (diazepam barbiturate association), on the sixth day of hospitalisation, and the second one after recovery. The comparison of the results shows that before PLT the mean arterial pressuure (MAP) is significantly increased (p less than 0.05) in patients after recovery in comparison to the same patients undergoing therapy. After PLT there is no significant difference between the two groups. Lastly, the variation of the left ventricular function points under PLT shows no significant difference between patients during therapy and after recovery. These results suggest that the diazepamphenobarbital combination does not alter the left ventricular function of the patients undergoing this therapy during tetanus.
Diazepam↗
Cyclic peptides. 19. Cation binding of a cyclic dodecapeptide cyclo-(L-Val-Gly-Gly-L-Pro)3 in an aprotic medium.
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Peptides, Cyclic↗
Detection and quantitation of human Ia-type antigens with iodinated protein A and specific purification of antibodies against Ia-type alloantigens.
Antibodies directed against specific human Ia-type antigens can easily be detected and quantitated by an improved radioimmunoassay using iodinated protein A bound to a specific antibody-Ia-antigen complex on the surface of freshly drawn peripheral human leukocytes, cultured human cell lines, or lymphoid cells fixed with glutardialdehyde or formaldehyde. The same principle can also be used for the detection of Ia alloantigens on human lymphocytes when testing them with specific antisera known to contain antibodies against transplantation antigens. These anti-Ia-alloantigen antibodies had been purified by a two-step procedure involving ion-exchange chromatography on DEAE-cellulose at pH 6.3 and the specific absorption on formaldehyde-fixed Ia-alloantigen-carrying homozygous cell lines, followed by elution of these antibodies with isotonic citrate buffer at pH 3.0. In this way an about 90-fold purification could be achieved. After such a purification the highly enriched antibody fraction still reacted selectively with one specificity of the Ia antigen system.
Cell Line↗
Effects of arsenic cell metabolism and cell proliferation: cytogenetic and biochemical studies.
Chromosome analysis of lymphocytes from patients who had been exposed to arsenic showed frequent structural and numerical aberrations, even with an interval of decades since the last exposure. The in vitro addition of sodium arsenate induced the same chromosome changes--even to extreme of chromosome pulverizations--upon lymphocyte cultures from healthy subjects. Radioactive incorporation studies showed that arsenate was able to inhibit dose-dependently the incorporation of radioactively labeled nucleotide in RNA and DNA. Beyond that, arsenic blocked the cells in the S- and G2-phase. A general explanation for the inhibitory effect of inorganic arsenic on cell metabolism is the known strong affinity of arsenic to enzymes, especially to those containing sulfhydryl groups.
Aged↗
[Aortic valvular rupture treated by suture in a case of multiple injuries].
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Adult↗
[Insulin-dependent diabetes and HLA].
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Adolescent↗
Comparative studies of the incorporation inhibition of radioactive nucleotides and amino acids in vitro under the influence of adriamycin, bleomycin, and sodium arsenate.
The incorporation of 14C-marked nucleotides and amino acids into the nucleic acids and into the cellular protein of PHA-stimulated human lymphocytes was inhibited variably by increasing doses of Adriamycin, Bleomycin and Na2HAsO4. The findings, which were obtained with the aid of the liquid scintillation counter and partly by means of 14C-thymidine-marked autoradiographies shows clearly that Adriamycin causes the strongest incorporation inhibition. In contrast to this, the inhibition caused by Bleomycin and arsenate is considerably less destructive. It was shown by means of cytophoto metrical examinations that Adriamycin is capable of fixing cells in the G2 phase and in the S phase. Cells react similarly to Bleomycin and arsenate. The differential inhibition of nucleic acid and protein metabolism induced by Adriamycin, Bleomycin and arsenate is correlated with a varying responsiveness of lymphocytes to the cytostatically effective substances.
Arsenates↗
The use of tombramycin in the management of severe infections. Clinical and pharmacological data.
Tobramycin was used in the treatment of 35 severe infections. Its clinical effectiveness was confirmed in broncho-pulmonary infections without septicemia and in septicemia without lung involvement. Poor results were obtained in septicemia where the initial site 9 infection was in the lungs. This antibiotic appeared as a very good antistaphylococcal agent. In vitro superiority over gentamicin against Pseudomonas was not be confirmed clinically. Tobramycin deserves to be administered initially in serious infections because of the possibility that the causative organism might be a gentamicin-resistant, tobramycin susceptible strain. Three such cases were observed in our 35 patients. This susceptibility dissociation in favor of tobramycin was demonstrated in two strains of Klebsiella and one strain of Enterobacter. A dosage regimen in patients with impaired renal function is proposed. It requires confirmation.
Acute Kidney Injury↗