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D Barbieri

Publications and source records attributed to D Barbieri.

125 records · Page 7Linked to original sources
PubMedDate not supplied

[Immunohistochemical study of colonic mucosa macrophages in children with Crohn's disease and ulcerative colitis].

In this research the histological characteristics of the macrophages on the colonic mucosa in Crohn's disease and ulcerative colitis were quantified and analysed. Twelve Crohn's disease, 19 ulcerative colitis and 10 specimen of the rectal mucosa, representing the control group according to the followed model, were studied: I period (PI) = pre-treatment, II period (PII) = up two years of evolution and III period (PIII) = more than two years of evolution. The macrophages were identified in a colonic mucosa by the monoclonal CD68 through the immunoperoxidase method. The macrophages quantification was done by chromatic computer images analysis, that express the area (mm2) used by the CD68 positive cells, in percentage. The percentage of the area used by the macrophages was increased in both diseases, in all the studied periods, when compared with the control group, but without statistic significance. The macrophages' distribution inside the control group mucosa was subepithelial, while in the illness group, it reached all the mucosa that was concentrated on the basis of ulcers and all long the fissures. On the Crohn's disease the CD68 positive cells facilited the identification of the microgranulomas, sometimes unnoticed in the hematoxiline-eosine. Although there was no difference between patients and control group in the macrophages area, the difference in the distribution could suggest the macrophages' participation on the injure in both diseases although they do not permit a differential diagnosis because of the variety of the values. The CD68 did not identify the different functional status of the macrophages, but their position in the mucosa suggest that, in terms of fissures and ulcers, their mainly function should be the phagocitosis and in the other cases, they have been the cells that should show the antigens and that recruit the other inflammatory cells.

Adolescent↗
PubMedDate not supplied

Variation and physiological significance of basal gastrinemia in normal children.

The objective of the present study was to determine the variation of normal basal gastrinemia values in children of different age ranges. Basal gastrinemia levels were measured by radioimmunoassay in 30 normal children aged 2 months to 9 years divided into 3 groups: 1) 11 children aged 2 to 6 months; 2) 9 children aged 7 to 18 months, and 3) 10 children aged 20 month to 9 years. Basal gastrinemia levels varied from 11 to 533 pg/ml (mean = 138.47, SD = 131.56) and correlated significantly to the children's ages. Differences were not statistically significant between group I (mean = 191.82, SD = 116.26) and group II (mean = 186.11, SD = 161.65) but were significant between group I and group I and group III (mean = 36.9, SD = 17.67) and between group II and group III. The results demonstrate that basal gastrinemia levels of normal children are inversely correlated with age, the highest values being observed up to 18 months of age. A marked decrease in basal gastrinemia occurs thereafter reaching levels similar to those reported for adults. The authors speculate whether the hypergastrinemia observed in the present study may be indicative of an important physiological function of gastrin in the ontogenic development of the stomach.

Age Factors↗
PubMedDate not supplied

[Study of serum antibody antigliadin of the immunoglobulin-A class in celiac disease].

Serum antigliadin IfA class antibodies (AAG-IgA) were measured by ELISA assay in 65 children into three groups. Group I consisted of 20 control children; Group II of 31 celiac patients on different treatment periods (P1, on a normal diet; P2, after sixth month on gluten-free diet; P3, one three years of treatment and P4, three years and one month to nine years of treatment) and Group III of 14 food hypersensitivity patients. IgA-deficiency was ruled out in all patients. Positivity was considered for titers > 30.3 UA obtained from controls. The AAG-IgA titer was positive in 9 of 10 celiac patients on P1. Nine of these 10 patients were tested on P2; all cases showed a decrease in the AAG-IGA level and a significant difference was observed between the mean values for P1 and P2; although seven patients had maintained positive titers. On P3 and P4 all patients had AAG-IGa negative levels. On P1 the AAG-IGA liter levels were significantly higher than the values for P2, P3 and P4. The AAG-IgA values decreased towards control levels after one year of treatment. In Group III one out of 14 patients had a positive titer and the AAG-IgA values in this group were significantly lower than the celiac titers on P1 and P2 and no difference was found compared to patients titers on P3 and P4. The sensitivity of ELISA test was 90% and the specificity 93%. The AAG-IgA determination proved to be reliable for differentiating celiac disease from food hypersensitivity and useful in monitoring gluten-free diet compliance.

Autoantibodies↗
PubMedDate not supplied

[Immunohistochemical study of HLA-DR expression in superficial epithelium and in lamina propria of colonic mucosa in children with Crohn's disease and nonspecific ulcerative colitis].

Some researches have stressed the importance of expression HLA-DR on the superficial epithelium and on the lamina propria of the colonic mucosa of patients with ulcerative colitis and Crohn disease. In this research the histological characteristics of the expression HLA-DR on the resident mucosa of both these illness were quantified and analysed. Twelve Crohn disease, 19 ulcerative colitis and 10 specimen of the rectal mucosa, representing the control group according to the following model were studied: I period = pre-treatment, II period = up two years of evolution and III period = more than two years of treatment. The expression HLA-DR was identified by the monoclonal HLA-DR (DAKO) in the resident mucosa specimen by the immunoperoxidase method. The quantification of the expression HLA-DR on the lamina propria was done through the chromatic computer images analysis that express the area (micron2) used by the positive HLA-DR cells and the superficial epithelium in percentage by a semi quantifier evaluation. On the lamina propria of the illness mucosa, the expression HLA-DR was increased in all the studied periods when compared with the control group, but without statistics differences. The distribution of the expression HLA-DR on the lamina propria was done to the corresponding localization to the macrophages and to the B lymphocytes. The superficial colonial epithelium of the ulcerative colitis and Crohn disease patients was in 86.95% and in 80.96% respectively HLA-DR positive in the three periods. The superficial epithelium of all the rectal mucosa of the control group did not show the HLA-DR expression. The presence of the HLA-DR expression on the superficial colonial epithelium of the ulcerative colitis and Crohn disease patients and its absence in the control group was a very important found in this research. It is a consense among several authors that the HLA-DR expression represents a intense local action of the inductorious cytokines of this expression in the colonic mucosa of the ulcerative colitis and Crohn disease patients, representing its activation in a answer to the invasor antigen in the intestinal mucosa. The study of the HLA-DR expression in the lamina propria was not conclusive, because the result did not represent a significative difference in relation with the control.

Adolescent↗