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D B Smith

Publications and source records attributed to D B Smith.

377 records · Page 21Linked to original sources

The effects of leg angular velocity on mean power frequency and amplitude of the mechanomyographic signal.

The purpose of the present investigation was to examine the effects of leg angular velocity on the mean power frequency (MPF) and amplitude of the mechanomyographic (MMG) signal during maximal concentric (CON) isokinetic muscle actions. Sixteen adult subjects performed maximal CON leg extensions on a calibrated Cybex 6000 dynamometer at leg angular velocities of 60 and 300 degrees.s-1. MMG was detected by a piezoelectric crystal contact sensor placed over the mid-portion of the vastus lateralis muscle. The results indicated a significant (p < 0.05) velocity-related decrease in peak torque (PT) and increase in MMG amplitude from 60 to 300 degrees.s-1. There was, however, no velocity-related change (p > 0.05) in MMG MPF. These findings did not support our hypothesis that increases across velocity in MMG amplitude were due to decreases in muscle stiffness as a result of a shift in the contribution of slow and fast-twitch muscle fibers to PT production. Future research should examine the potential influence of actin-myosin cycling rate as well as limb movement on the MPF and amplitude of the MMG signal.

Actins↗

Defined substrate technology method for rapid and specific simultaneous enumeration of total coliforms and Escherichia coli from water: collaborative study.

The defined substrate technology (DST) method is a reagent system designed to enumerate specific target microbes(s) from a mixture of bacteria. The system simultaneously enumerates total coliforms and Escherichia coli directly from a water sample. The reagent contains o-nitrophenyl-beta-D-galactopyranoside (ONPG), which is hydrolyzed by total coliforms to produce a yellow chromogen, and 4-methylumbeilliferyl-beta-D-glucuronide (MUG), which is hydrolyzed and fluoresces when E. coli organisms grow. Noncoliform bacteria are suppressed and cannot metabolize the indicator nutrients. Nine laboratories participated in a field evaluation of the method, which covered a wide range of surface and subsurface water sources and water-processing modalities, including the examination of natural samples. The DST system was compared to multiple-tube fermentation (MTF) (quantitative) and presence-absence (P-A) (qualitative) Standard Methods formats. Comparison of water samples from natural sources by using the most probable number (MPN) procedure showed that the DST test was equivalent to the currently used MTF test. Results from the DST and the qualitative P-A procedure showed that these tests agreed with each other in 94% of the water samples analyzed. Specificity of the DST method was established by subculturing a species consistent with a total coliform or E. coli from each positive tube. Eight laboratories participated in a collaborative study of the method. Each laboratory received 3 concentrations of E. coli (organisms/100 mL); 10 (low); 60 (medium); and 120 (high). The DST test was inoculated from a split sample of each bacterial density in parallel with Standard Methods brilliant green lactose broth.(ABSTRACT TRUNCATED AT 250 WORDS)

Escherichia coli↗

Missing fall.

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Adult↗

A moment in time.

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Colostomy↗

Moments in time.

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Enterostomy↗