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Biomedical subjects

D B Orr

Publications and source records attributed to D B Orr.

9 recordsLinked to original sources

Evaluation of a commercially available beneficial insect habitat for management of lepidoptera pests.

A field study was conducted in 2003 and 2004 at the Center for Environmental Farming Systems in Goldsboro, NC, to evaluate the effectiveness of a commercially available beneficial insect habitat in decreasing pest caterpillar populations in organically managed tomato, Lycopersicon esculentum Mill., plots. Six pairs of tomato plots were established and a commercial beneficial insect habitat seed mix (Peaceful Valley's Good Bug Blend) transplanted around the perimeter of treatment plots, whereas a brown-top millet, Brachiaria ramose (L.) Stapf., border was planted around control plots. Egg predation, egg parasitism by trichogrammatid wasps, and larval parasitism by braconid wasps was monitored throughout the growing season to determine whether habitat increased their activity. In both years of this study, the density of Helicoverpa zea (Boddie) and Manduca spp. eggs was not significantly different between treatment and control plots. Although parasitism was the most important component of egg mortality (19-49%), parasitism was not significantly different between habitat types. Identifiable predation was a minor component (3-9%) of egg fate; it is possible that unidentified predation may be part of the approximately 35-52% of eggs that met unknown fates. Larval parasitism levels ranged from approximately 10 to 90% but was not significantly influenced by the presence of beneficial insect habitat in either year of the study. These results demonstrate that natural enemy activity in organic tomatoes was not amplified, and pest populations were not reduced by the presence of a commercially available beneficial insect habitat.

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Effect of insecticides on Trichogramma exiguum (Trichogrammatidae: Hymenoptera) preimaginal development and adult survival.

The effect of insecticides on Trichogramma exiguum Pinto & Platner emergence, adult survival, and fitness parameters was investigated. Insecticides tested were lambda cyhalothrin, cypermethrin, thiodicarb, profenophos, spinosad, methoxyfenozide, and tebufenozide. All insecticides, with the exception of methoxyfenozide and tebufenozide, adversely affected Trichogramma emergence from Helicoverpa zea (Boddie) host eggs when exposed at different preimaginal stages of development (larval, prepupal, or pupal). Regardless of the developmental stage treated, none of the insecticides tested had a significant effect on the sex ratio or frequency of brachyptery of emerged females. However, the mean life span of emerged T. exiguum females significantly varied among insecticide treatments, and was significantly affected by the developmental stage of parasitoid when treated. Based on LC50 values, spinosad and prophenofos were the most toxic compounds to female T. exiguum adults, followed by lambda cyhalothrin, cypermethrin, and thiodicarb. Insecticides field-weathered for four to 6 d on cotton leaves showed no activity against female T. exiguum adults.

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Trichogramma exiguum (Hymenoptera: Trichogrammatidae) releases in North Carolina cotton: evaluation of heliothine pest suppression.

Field studies were conducted in 1996 and 1997 to reevaluate the use of augmentative releases of Trichogramma wasps for heliothine management in cotton. In 1996, nine releases of Trichogramma exiguum Pinto & Platner, spaced 3-4 d apart, were made into three 0.4-ha cotton plots. Six weekly releases were made in 1997, each containing two T. exiguum cohorts developmentally staggered by 45 degrees C degree-days. Field release rates, estimated from laboratory and field quality control data, averaged 108,357 T. exiguum female female per hectare per cohort per release in 1996 and 193,366 female female per hectare per cohort per release in 1997. In 1996, mean +/- SD adult emergence under laboratory conditions for released cohorts was 92 +/- 7%; 62 +/- 5% of emerged adults were females, 3 +/- 2% of females displayed brachyptery (nonfunctional wings), mean female longevity under laboratory conditions was 15 +/- 4 d, and mean +/- SD field emergence was 97 +/- 2%. Quality control measurements were similar in 1997. In 1996, mean +/- SD percent parasitism of heliothine eggs in field plots on the sampled dates ranged from 67 +/- 4 to 83 +/- 5% in T. exiguum release plots and 25 +/- 9 to 55 +/- 8% in control plots. In 1997, parasitism levels ranged from 74 +/- 4 to 89 +/- 5% in T. exiguum release plots and 18 +/- 18 to 69 +/- 11% in control plots. Despite increased parasitism levels in T. exiguum release plots, there were no significant differences in density of fifth instars, boll damage, or yield between T. exiguum release and control plots. Therefore, it is concluded that Trichogramma augmentation is not an effective heliothine management tool in North Carolina cotton.

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Trichogramma releases in North Carolina cotton: why releases fail to suppress heliothine pests.

Field studies were conducted in 1996 and 1997 to determine the fate of naturally oviposited F3 heliothine eggs in cotton plots treated with augmentative releases of Trichogramma exiguum Pinto & Platner and nontreated plots. Four cohorts of newly oviposited eggs (< 24 h old) were followed in 1996 and two cohorts in 1997. In 1996, mean +/- SD percent parasitism, estimated by in-field studies following the fate of naturally oviposited eggs, ranged from 7 +/- 7 to 61 +/- 8% in T. exiguum release plots and 0 +/- 0 to 35 +/- 13% in control plots. The mean +/- SD percent of eggs hatched in T. exiguum release plots ranged from 1 +/- 2 to 11 +/- 4% and 7 +/- 4 to 28 +/- 10% in control plots. In 1997, mean +/- SD percent egg parasitism ranged from 27 +/- 4 to 40 +/- 3% in T. exiguum release plots and 15 +/- 18 to 25 +/- 8% in control plots. The mean +/- SD percent of eggs hatched in T. exiguum release plots ranged from 7 +/- 3 to 12 +/- 2% and 18 +/- 6 to 28 +/- 8% in control plots. Despite increased parasitism and reduced egg hatch in T. exiguum release plots, overall, there was no significant difference in larval density (all instars combined) between T. exiguum release and control plots. Combined analysis of the heliothine larval populations and egg fate data revealed that the additional egg mortality produced by released T. exiguum was offset by lower larval mortality in release plots. Because of the occurrence of compensatory mortality, the egg stage of heliothines is not an appropriate target for biological control using Trichogramma wasp releases.

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