Biomedical subjects
Publications and source records attributed to D B Dzhusupova.
The work was aimed at studying alpha-methylstyrene oxidation by three active Pseudomonas cultures. P. acidovorans 9 oxidized alpha-methylstyrene only to acetophenone whereas two P. aeruginosa cultures assimilated the compound entirely. Analysis of the intermediate products and enzymes catalyzing the aromatic ring cleavage suggests that these strains oxidize alpha-methylstyrene via a new unknown pathway.
Pseudomonas acidovorans 9 transforming alpha-methylstyrene into acetophenone contains four types of plasmid DNA with molecular masses of 130, 110, 36 and 54 MD. The loss of the "growth on alpha-methylstyrene" property by this strain correlates with the absence of plasmids with the molecular masses of 130 and 110 MD from the cells. All the types of plasmid DNA are found in transconjugants growing on alpha-methylstyrene and produced by crossing the parent P. acidovorans strain with the plasmidless variant of this strain incapable of alpha-methylstyrene transformation. Apparently, plasmids with the molecular masses of 130 and 110 MD participate in the genetic control of alpha-methylstyrene transformation into acetophenone by P. acidovorans 9.
Two bacterial strains assimilating alpha-methylsterene as a sole carbon and energy source were isolated from the sewage of an industrial plant producing synthetic rubber. The morphological, cultural and physiologo-biochemical properties of the strains were studied and their taxonomic position was determined. One of the cultures was classified as Bacillus cereus and the other as Pseudomonas aeruginosa. The rate of alpha-methylsterene destruction in the chemically defined medium was shown to depend on the conditions of cultivation.