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D B Anderson

Publications and source records attributed to D B Anderson.

48 records · Page 3Linked to original sources
PubMed1980

Rate of fat compensation and growth efficiency of lipectomized Sprague Dawley rats.

The objective of this experiment was to measure changes in food efficiency and to determine the rate of fat compensation following the surgical removal of adipose tissue in Sprague Dawley rats. The right inguinal fat pad and both epididymal pads were surgically removed from 375 g male castrate rats that were then paired with sham-operated controls. Pairs were evenly distributed into one of five groups. The rats were killed when they reached their group designated weight: immediate post surgery, 375, 405, 435 and 465 g. Dissected fat weight was significantly less in lipectomized animals at all body weights except 465 g. Gradual fat compensation up to 465 g was due to partial regeneration of the right inguinal fat pad as well as compensatory growth of other (intact) fat depots. Epididymal fat pads showed no signs of regeneration. Lipectomized animals had similar food intake but gained weight more rapidly than shams. Lipectomized rats had more food available for production when calculated maintenance energy requirements were subtracted from total food intake. Efficiency of utilization of production food did not differ significantly between treatments. Compensatory weight gain seen in lipectomized rats was therefore due to greater ad-libitum food consumption above maintenance requirements.

Adipose Tissue↗
PubMed1980

Relative growth rates of various fat depot in Sprague Dawley rats.

Seven major internal and subcutaneous fat depots were dissected from male Sprague Dawley rats at various weights from 150 g to 500 g. Tissue growth rates relative to body weight were calculated. With the exception of the dorsoscapular fat depot, the growth coefficients for all fat depots were as expected, greater than 1, indicative of later developing tissues. The order of development from earliest to latest, based on increasing growth coefficients, was dorsocapsular, mesenteric, axillary, buttock, epididymal, inguinal and perirenal. This differs from other species where the perineal depot is one of the earliest developing tissues.

Adipose Tissue↗
PubMed1978

The effects of lipectomy on remaining adipose tissue depots in the Sprague Dawley rat.

Adult male castrate Sprague Dawley rats were subjected to surgical removal of the right inguinal and both epididymal fat depots. Lipectomized rats were not significantly different from controls in total body fat at approximately 13 weeks after surgery suggesting a compensatory fat gain in response to the lipectomy. Mean wet weight of the major individual fat depots dissected from lipectomized rats exceeded those of the control rats with the exception of surgically excised depots. The generalized trend for the adipose tissue mass to compensate in response to lipectomy was in part due to a partial regeneration of the excised inguinal fat depot. It was observed that the cellular nature of the fat compensation was dependent on the location of the tissue. The major internal depots compensated by a significant increase in average fat cell size, whereas the major subcutaneous depot (inguinal) compensated by a significant increase in the apparent fat cell number with no change in cell size.

Adipose Tissue↗
PubMed1973

On certification.

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Certification↗
PubMed1973

Cellular and enzymatic changes in porcine adipose tissue during growth.

Experiments were designed to define some of the cellular and metabolic changes in various areas of porcine adipose tissue during growth and to establish a relationship between these changes and the accumulation of fat in the domestic pig. 35 male castrate pigs were killed at various ages from late fetal to 6.5 months. The following determinations were made on each animal: (1) total carcass fat, (2) adipose cell size and number by fixation of adipose tissue with osmium tetroxide, and (3) the activities of acetyl CoA carboxylase, citrate cleavage enzyme, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and malic enzyme from perirenal adipose tissue and each of the three layers of subcutaneous backfat. Carcass adipose tissue expanded by a combination of adipocyte hyperplasia and hypertrophy up to 5 months, after which adipose expansion was accomplished by cellular hypertrophy only, with no significant increase in cell number. The activities of the selected lipogenic enzymes (expressed on an adipose cell basis) increased markedly at weaning and again during the rapid increase in percentage of body fat between 3.5 and 5 months. Enzyme activities reached a peak at 5 months, after which activities decreased to values approaching mature levels.

ATP Citrate (pro-S)-Lyase↗
PubMed1972

Lipogenic enzyme activities and cellularity of porcine adipose tissue from various anatomical locations.

The activities of acetyl CoA carboxylase, citrate cleavage enzyme, malic enzyme, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase were determined in porcine adipose tissue samples taken from seven anatomical locations, including three layers of backfat, intermuscular, perirenal, mesenteric, and leg subcutaneous adipose tissues. Adipocyte size and number, as well as lipid and soluble protein content, were also measured in order to establish some of the differences that exist between different areas of porcine adipose tissue. It was found that adipose tissue from areas where fat is deposited very readily (particularly the perirenal region) had higher enzyme activities, larger adipose cells, a lesser amount of stromal tissue, a greater amount of ether-extractable lipid, and a lower concentration of adipose cells per gram of tissue than samples from areas where fat is deposited only sparsely (leg subcutaneous).

Adipose Tissue↗