Search PubMed⌕ Search

Biomedical subjects

D B Amos

Publications and source records attributed to D B Amos.

At least 91 records · Page 5Linked to original sources
PubMed1977

HLA and ragweed allergy.

In summary, two large families were subjected to genetic analysis of the distribution of the immune response. S linkage analysis indicated that this locus for positive skin test reaction to antigen E and/or personal history or seasonal allergy is linked to HLA-B. The maximum likelihood estimate of the recombination frequency is 0.13 in males and 0.53 in females, with a lod score of 2.53.

Cytotoxicity Tests, Immunologic↗
PubMed1976

Stimulation of lymphocytes by allogeneic lymphocytes and lymphoblasts in the presence of anti-HLA antisera.

Anti-HLA alloantisera inhibit mixed lymphocyte responses in which normal lymphocytes are used as stimulator cells. These same antisera are unable to inhibit lymphocyte proliferative responses stimulated by lymphoblastoid cells from cultured lymphoid cell lines. They also fail to inhibit either the generation of cytotoxic effector cells by lymphoblastoid cells or lymphocyte-mediated cytotoxicity against the lymphoblasts. Although the number of HLA antigens on the surface of lymphoblasts is reported to be greater than on normal lymphocytes, the failure of alloantisera to inhibit lymphoblast-induced responses in vitro does not appear to be due to insufficient amounts of antiserum to react with the antigenic sites. Rather, the data are interpreted to suggest that antigens which are not HLA and are not closely associated with HLA on the lymphocyte membrane are responsible for the stimulation of allogeneic lymphocytes by lymphoblastoid cells. Although lymphoid cell lines are known to contain the genome of the Epstein-Barr virus, antisera against products of the viral genome fail to inhibit proliferative responses to lymphoblastoid cells, suggesting that these antigens do not directly participate in lymphocyte activation.

Antibodies, Viral↗
PubMed1976

A simple method for screening human T and B lymphocyte alloantibodies.

An sRBC-hemolysin monolayer was employed for separating human lymphocytes into two sub-populations, adherent cells (predominantly B cells) and non-adherent cells (predominantly T cells). The separated cells were then tested simultaneously against predispensed sera by complement-mediated cytolysis for the presence of alloantibodies to either T or B cells. A total of 372 sera were screened. Antibodies specific for B cell antigens were found in 19 sera and 9 sera were found to contain antibodies to T-specific antigens.

B-Lymphocytes↗
PubMed1976

Isoelectric focusing of an anti-HL-A2 antiserum cross-reacting with W28.

The cytotoxicity activity of an anti-HL-A2 antiserum cross-reactive with W28 appeared in three distinct peaks after the serum was subjected to isoelectric focusing. The pH at which the peaks formed was reproducible using pH 5-8 or pH 3.5-10 gradients. Anti-HL-A2 activity occurred in each peak, but the cross-reactive anti-W28 cytotoxicity appeared to be largely confined to the second and third peaks. The first peak was exclusively IgG, the second contained IgG and IgM, and the third contained IgG, IgA, and IgM.

Cross Reactions↗
PubMed1976

The genetics of cell-mediated lympholysis.

The role of HLA antigens in the generation of cytotoxic cells in CML has been investigated. Cytotoxic effector cells were generated in MLC among HLA-A or HLA-A and HLA-B disparate, HLA-D identical siblings, and among HLA-A and HLA-B disparate, MLC identical (%RR less than or equal to 2 3.6) unrelated individual. The data indicate that HLA-D differences and poliferative MLC responses as measured by 3H-thymidine incorporation are not requisite for the in vitro generation of cytotoxic cells and suggest the existence of a CML-S locus (loci) distinct from HLA-A, HLA-B and HLA-D. The degree of cytotoxicity generated in a proliferative versus a "nonproliferative" MLC was comparable. In addition, these studies demonstrate that antigens other than the currently definable HLA-A, HLA-B, HLA-C, and HLA-D can serve as target determinants in cell-mediated lympholysis.

Chromosome Mapping↗
PubMed1975

Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera.

Human sera were tested for cytotoxicity to pairs of long-term tissue-cultured cell lines. Each pair had been derived from the same individual and one of the pairs possessed the characteristics of either "T" or "B" cells. The alloantisera used were HL-A-typing reagents or sera obtained from Amish multiparas. Selected cytotoxicity was found against the B-cell lines by direct testing. Cytotoxicity was abolished by absorption with B-cell line but not by absorption with the T-cell lines. The results suggest that a group of allotypic antigens may be expressed exculsively on human B cells.

B-Lymphocytes↗
PubMed1975

Specific human B lymphocyte alloantigens linked to HL-A.

Sera, previously found to react specifically with B lymphoid cultured cells, were tested on isolated T and B peripheral blood lymphocytes in a microcytotoxicity assay. Studies were performed on lymphocytes obtained from several large Amish families. The sera used in these studies were cytotoxic to peripheral blood, B lymphocytes, but not cytotoxic to T lymphocytes. The antigens detected followed the inheritance pattern of HL-A haplotypes. The strong linkage disequilibrium with HL-A antigens suggests that genes controlling the expression of B lymphocyte antigens are linked to genes controlling HL-A alloantigens.

B-Lymphocytes↗