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Biomedical subjects

D Allen

Publications and source records attributed to D Allen.

At least 271 records · Page 15Linked to original sources
PubMed1984

Zinc intakes of lactating and nonlactating women: analyzed vs. calculated values.

The zinc intake of a group of 23 lactating and 13 nonlactating postpartum women was assessed longitudinally from 37 weeks' gestation through six months postpartum. Dietary zinc was determined by chemical analysis of three-day duplicate food and beverage composites and by calculation of three-day records. There was no significant difference between the analyzed and calculated values for daily zinc intake. Both groups of postpartum women consumed 42% or less of the Recommended Dietary Allowance (RDA) for zinc. To attain the RDA within present food selections, both groups of postpartum women would need to eat unreasonably high amounts of calories and protein.

Adult↗
PubMed1983

Deoxyribonucleic acid (DNA) content of carcinomas and prognosis in human breast cancer.

DNA content was estimated in 705 breast carcinomas from patients with stage I or stage II disease undergoing mastectomy, to investigate whether it would predict the clinical course of the disease. The patients were then followed for up to 84 months during which time 166 of them developed recurrences. There were no statistically significant differences in the DNA content of carcinomas between stage I and stage II patients or between those with various numbers of involved nodes. The tumours from pre-menopausal patients as a group had significantly higher DNA content than those from post-menopausal cases. There was a gradual rise in DNA content with the malignancy grade of the carcinoma which showed significant differences between Grades I and III and between II and III. Life-table analyses showed that the measurement of DNA would not be an aid to prognosis. Furthermore, when the data were stratified according to menopausal status, stage, malignancy grade or numbers of lymph nodes involved, the results indicated that these estimation do not add significantly to the information obtained through established prognostic factors. Survival data were available on III patients with recurrent disease. There were no significant differences in tumour DNA content between short- and long-term survivors. It is concluded that these measurements seem unlikely to be a prognostic factor in human breast cancer.

Adult↗
PubMed1983

Maternal and child characteristics associated with mothers' perceptions of their high risk/developmentally delayed infants.

Mothers of 46 high risk infants, many of whom were developmentally delayed, were visited in their homes at approximately 9 months post-expected-date-of-delivery. The Perception of Baby Temperament (PBT) scales and the Profile of Mood States were administered and an observation of infant, parent, and parent-infant behavior was made. Infants were most likely to receive higher PBT scores on more than one scale if their mothers were more highly educated and reported lesser degrees of depression and anxiety. Infants who were perceived as more active and approaching by their mothers tended to be rated as more responsive by the observer and to show less developmental delay. Mothers who rated their infants as more active tended to show greater responsiveness to the infant and to participate in reciprocal activities with the infant more frequently during the home visit. These findings demonstrate that mothers' perceptions of high risk/developmentally delayed infant temperament are associated with similar categories of variables found to correlate with maternal perceptions of developmentally normal infant temperament.

Adaptation, Psychological↗
PubMed1983

High- and low-affinity binding of [3H]imipramine in mouse cerebral cortex.

Binding of [3H]imipramine in mouse cerebral cortex was found to be nonhomogeneous. Competition experiments, Scatchard analysis, and Hill plots are compatible with the existence of binding with high (nanomolar) and low (micromolar) affinity. Low-affinity binding could be eliminated by the use of low concentrations of imipramine as the competing ligand. In contrast to the high-affinity binding, the low-affinity binding was found to be unrelated to the neuronal uptake system for serotonin.

Animals↗
PubMed1982

Cost of counselling women who undergo mastectomy.

The psychiatric morbidity associated with mastectomy and brest cancer was substantially reduced by a specialist nurse who counselled women before and after surgery and monitored their progress. A controlled study was, therefore, carried out to determine what this cost. National Health Service costs were almost wholly covered by savings made because counselled subjects who developed psychiatric problems were recognised and treated much earlier than control patients. Counselled and control subjects suffered considerable financial losses, but these were offset in the counselled group by their relatives' earlier return to work. Such counselling schemes are necessary and effective and may be implemented at little extra cost.

Community Health Services↗
PubMed1982

Biosynthesis of cell surface sulfated glycoproteins by cultured vascular endothelial cells.

Cultured vascular endothelial cells incorporate 35SO4 into a class of oligosaccharides which are N-glycosidically linked to cell-surface or extracellular proteins. This type of sulfated oligosaccharide was not synthesized by smooth muscle cell cultures and may represent sulfation of vascular tissue glycoproteins which are unique to endothelial cells. These endothelial cell 35S-labeled oligosaccharides are not released from the polypeptides under alkaline conditions that cleave O-glycosidically linked chains. The incorporation of both [3H]hexosamine and 35SO4 into these oligosaccharides is inhibited by tunicamycin, an antibiotic which inhibits protein N-glycosylation. 35S-Labeled oligosaccharides, isolated as glycopeptides, were resistant to a variety of chemical and enzymatic treatments which degraded vascular cell sulfated glycosaminoglycans but appear to contain terminal sialic acid residues. Endothelial cell sulfated oligosaccharide chains could also be distinguished from sulfated glycosaminoglycans by the apparent size and charge. Endothelial cells released or secreted 35S-labeled glycoproteins into the culture medium. These soluble 35S-labeled glycoproteins were partially purified by DEAE-cellulose chromatography and separated from the 35S-labeled proteoglycans, which are also released into the medium. The major 35S-labeled glycoproteins released from the cells had apparent molecular weights of 24 000--66 000. The cell-associated 35S-labeled glycoproteins, released with ethylenediaminetetraacetate or extracted with 0.5% octyl glucoside, were partially purified on DEAE-cellulose and had apparent molecular weights of 45 000--250 000.

Animals↗
PubMed1982

Relation of concentrations of Haemophilus influenzae type b in cerebrospinal fluid to late sequelae of patients with meningitis.

Forty-four patients with Haemophilus influenzae type b meningitis had follow-up evaluations approximately one year after hospital discharge. Patients with greater than or equal to 10(7) colony-forming units of H. influenzae type b per milliliters CSF had significantly greater frequencies of speech impairment (P less than 0.001), hearing loss (P = 0.04), and moderate or severe neurologic sequelae (P less than 0.01). Patients with greater than or equal to 1 microgram H. influenzae b antigen/ml CSF had a greater incidence of hearing loss (P = 0.03) but not of speech abnormalities (P = 0.06) or other neurologic sequelae (P = 0.64). Glucose concentrations less than 10 mg/dl correlated with the incidence of hearing loss (P = 0.02) and speech impairment (P = 0.02). "Partial" antibiotic therapy, CSF protein concentrations, and number of CSF polymorphonuclear leukocytes did not correlate well with sequelae. These data indicate that pretreatment concentrations of H. influenzae b and glucose concentrations in CSF were the best predictors of late sequelae of patients with H. influenzae b meningitis.

Adolescent↗
PubMed1982

A method for freezing sheep lymphocytes prior to cytotoxicity testing.

The two stage freezing technique was adapted for use with sheep lymphocytes. Parameters investigated were lymphocyte concentration, composition of thawing medium and DMSO concentration. The main difference between this and earlier published techniques is the use of a high DMSO concentration (17%). The technique adopted was both simple and reliable. It consistently gave lymphocyte viabilities of 95% or more in thawed cell suspensions. The procedure is apparently without effect on lymphocyte antigen expression and thus appears very suitable for use in microlymphocytotoxicity tests.

Animals↗
PubMed1982

Home environments of developmentally disabled infants as a function of parent and infant characteristics.

Variables from three categories were inspected as correlates of HOME Inventory ratings for 43 severe perinatal risk or developmentally disabled infants at 8 to 9 months post-expected date of delivery. These variables were maternal perceptions of the infant's temperament, maternal self-reported mood, and interactional characteristics of the infant as rated by a home observer. Stepwise regression analysis of HOME total scores showed that infants who were viewed by their mothers as more active and by the observer as less irritable were particularly likely to experience more optimal developmental practices in the home. Significant predictors in regression equations for various of the HOME subscales were: maternal ratings of the infant's mood, approach, adaptability, and rhythmicity; maternal self-reported depression, tension, and confusion; and infant pleasure in physical contact. The finding that homes of fathers with high occupational status were rated lower on Maternal Involvement and Emotional and Verbal Responsivity of the Mother is inconsistent with results of studies of the homes of developmentally normal children.

Behavior↗
PubMed1982

Maternal causal attributions at hospital discharge of high-risk infants.

Interviews were conducted at hospital discharge with mothers of 43 infants with severe perinatal complications posing risks to development. Information gathered included mothers' beliefs about the causes of the infant's medical problems, expectations of caretaking difficulties, and self-reported mood on the Profile of Mood States. Of the various demographic characteristics studied, only maternal parity was related to causal attributions, with mothers of later-born infants more likely to blame themselves and mothers of first-born infants more likely to blame others and to attribute the infant's condition to chance. Mothers who blamed others reported greater mood disturbance and greater anticipated caretaking problems than did mothers who attributed the infant's condition to a maternal behavior during pregnancy. These findings were discussed in relation to studies of attributional processes and coping with misfortune.

Attitude↗