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Biomedical subjects

D Albrechtsen

Publications and source records attributed to D Albrechtsen.

At least 127 records · Page 7Linked to original sources
PubMed1979

Cell-mediated lymphocytotoxicity directed against HLA-D gene products.

Cytotoxic effector cells were generated in human mixed lymphocyte cultures (MLCs) between cell donors differing at the HLA-D/DR region but not at HLA-A,B(C). These cells killed Epstein-Barr virus-infected lymphoblasts (EBV blasts) from donors sharing the HLA-D/DR determinants with the normal MLC-stimulating cells, but not phytohaemagglutinin (PHA) lymphoblasts from the same donors. Because EBV blasts are derived from B cells and express HLA-D/DR antigens, the most likely interpretation of these findings is that the HLA-D/DR antigens may, by themselves, act as targets for cell-mediated cytotoxicity.

Cytotoxicity, Immunologic↗
PubMed1979

Significance of HLA matching in renal transplantation. A prospective one-center study of 485 transplants matched or mismatched for HLA-A, B, C, D, and DR antigens.

Matching for HLA haplotypes as well as for HLA-A and B antigens improved graft survival in 112 living related first transplants. In cadaveric first transplants, matching for HLA-A and B antigens had a beneficial effect on the fate of 373 grafts, while matching for HLA-C antigens had no predictive value. One hundred seventeen cadaveric transplants and their recipients were prospectively typed for the HLA-DR antigens. Compatibility for HLA-DR was found to be prognostically beneficial irrespective of matching for HLA-A and B antigens, and with no difference between transfused and nontransfused patients. Matching both for HLA-A , B and D/DR was thus found to influence the outcome of renal transplantation.

Adult↗
PubMed1978

Significance of HLA-D/DR matching in renal transplantation.

A low mixed-lymphocyte-culture response, indicating matching for HLA-D, was associated with a relatively good chance of graft survival in 62 recipients of renal transplants from living relatives mismatched for one or two HLA A and/or B antigens. In 96 cadaveric transplants prospectively typed for HLA-DR antigens, compatibility for these antigens improved the prognosis, irrespective of matching for HLA A and B antigens. In cadaveric transplants, a positive B-cell cross-match test before transplantation tended to predict inferior graft survival.

ABO Blood-Group System↗
PubMed1978

Stimulating capacity of human lymphoid cell subpopulations in mixed lymphocyte cultures.

The content and yield or B cells, T cells and monocytes were investigated in cell fractions separated from human peripheral blood by various techniques. These cell fractions, which were highly enriched for either B cells, T cells or monocytes, were tested for their capacity to stimulate allogenic peripheral blood mononuclear cells in mixed lymphocyte cultures (MLC). B cells were found to be strongly stimulatory. Monocytes stimulated strongly at low stimulator/responder cell ratios, while less stimulation was observed using larger amounts of cells. T cells were demonstrated to stimulate weakly, if at all.

B-Lymphocytes↗
PubMed1978

Relationship of HLA--D associated B lymphocyte (Ia-like) antigens in the cell membrane.

The molecular relationship of HLA--D-associated by B lymphocyte antigens was studied in lysostrip, immunofluorescence and MLC inhibition experiments. Two B cell antigens, strongly associated with HLA--Dw2 and Dw3 respectively, redistributed independently of each other in the cell membrane. A more broadly reacting B cell antiserum, which apparently recognized a public antigenic structure present on B cells of different HLA-D phenotypes, coaggregated with the HLA-DW3-associated B cell antigen. Antibodies directed against the HLA-Dw2 and-Dw3 associated cell antigens caused specific stimulating cell inhibition in MLC. Less.inhibition was observed if the target B cell antigen was shared between the responding and stimulating cell donor and thus did not constitute an allogenic difference, indicating that these antigens are MLC stimulating determinants and that they reside on separate molecules in the cell membrane.

B-Lymphocytes↗
PubMed1978

Strong association between the HLA-Dw3-related B cell alloantigen -DRw3 and coeliac disease.

The frequency of HLA-A, -B, and -C antigens, of -DRw3 and the associated B-lymphocyte alloantigen -DRw3 has been studied in 68 children with coeliac disease (CD). CD was found to be primarily associated with the B-lymphocyte alloantigen, the relative risk being calculated to 54. The possible relation between the B-lymphocyte alloantigen DRw3 and -Dw3 is discussed. The high relative risk and the fact that 95% of the patients with probable or definite CD had the B-lymphocyte alloantigen DRw3, underline the diagnostic value of B-lymphocyte typing in coeliac disease.

B-Lymphocytes↗
PubMed1977

Inhibition of human mixed lymphocyte culture stimulation by anti-HLA-D-associated antisera: studies with primed responding cells.

The inhibitory effects of HLA-D-associated antisera on stimulating lymphocytes when cultured together with allogeneic primed responding lymphocytes were investigated. The responding lymphocytes were primed for either the HLA-Dw2 or Dw3 determinants. The presence of HLA-D-associated (Ia-like) antibodies during the culture period specifically inhibited the stimulatory capability of lymphocytes possessing the HLA-D determinants with which the antisera were apparently reacting, as long as the stimulating cells carried the determinant for which the responding cells were primed. HLA-D-associated antisera of other specificities caused no decrease in the stimulatory capability. These antisera, therefore, apparently contain antibodies which are reactive with determinants closely associated or identical to the HLA-D determinants and may be human analogues to the mouse Ia antigens.

Binding Sites, Antibody↗
PubMed1977

HLA-D associated alloantisera react with molecules similar to Ia antigens.

Two human alloantisera specific to bone marrow-derived lymphocytes (B cells) were shown to precipitate polypeptide chains of 29,000 and 34,000 daltons from human lymphoblastoid B cell lines. These molecules are similar to murine Ia antigens and are also precipitated by a rabbit B-cell specific heteroantiserum. Since the alloantisera are thought to recognize determinants coded by HLA-D or closely linked loci, these data support the hypothesis that these B-cell specific alloantigens are the human counterpart of the mouse Ia antigens and may be the products of HLA-D.

B-Lymphocytes↗
PubMed1977

HLA-D-associated 'Ia-like' antigens on human macrophages.

Human macrophages procured by culture of adherent peripheral blood mononuclear cells were investigated for reactivity in complement-dependent cytotoxicity tests with antisera having specificity for HLA-D-associated 'Ia-like' B-cell antigens. The antisera were found to be cytotoxic for 30%-70% of the macrophages from donors whose B cells were also reactive, and absorption with B cells also removed macrophage cytotoxicity. A B-cell xenoantiserum was also cytotoxic to macrophages. It is concluded that the HLA-D-associated 'Ia-like' antigens present on B cells are also expressed by macrophages, at least on a subpopulation.

B-Lymphocytes↗
PubMed1977

Human Ia-like antigens associated with HLA-d.

Antisera raised with HLA-A- and -B-compatible, HLA-D-disparate combinations were cytotoxic to B lymphocytes from the immunizing donor's HLA-D phenotype. Four antisera recognized structures closely associated with the HLA-D determinants Dw2, Dw3, Dw4, and LD 108. One antiserum had a broad reactivity pattern, including Dw3, Dw6, and some unknown specificity(ies). In population and family studies these B-lymphocyte antigens behaved as if they were governed by one genetic locus in the B-D region of the HLA complex. Furthermore, the antisera were cytotoxic to a minor concavalin-A-reactive T-cell subpopulation. The antisera had previously been shown to inhibit the stimulating cells in mixed lymphocyte culture and to be capable of inhibiting the Fc receptor in the EA rosette assay. We conclude that the antisera produced by this method recognize Ia-like antigens closely associated with the HLA-D determinants.

B-Lymphocytes↗
PubMed1977

Stimulation of T lymphocytes by autologous non-T lymphoid cells. Participation of HLA-D?

Non-T lymphoid cells were found to be able to stimulate autologous T cells in vitro. This "autologous" response was shown to be about 20% of the response to allogeneic unfractionated lymphocytes. The ability to induce autostimulation appeared to be a property of the Ig-positive lymphocyte fraction. Blocking experiments with allosera recognizing HLA-D-associated determinants suggested that the HLA-D determinants present on the stimulating cells might be involved in the stimulation of autologous T cells.

Cell Fractionation↗
PubMed1977

Serological identification of five HLA-D associated (Ia-like) determinants.

Five antisera raised within HLA-A and -B compatible, HLA-D disparate combinations were reactive in a modified NIH microcytotoxicity test with B lymphocytes, but not with T lymphocytes from the immunizing donor, as well as with B lymphocytes from most or all donors sharing his immunizing HLA-D phenotype. Four antisera recognized structures closely associated with the HLA-D determinants Dw2, Dw3, Dw4 and LD 108. One serum had a broad reactivity pattern including Dw3, Dw6 and some unknown specificity(ies). In population and family studies, these B lymphocyte antigens behaved as if they were governed by one genetic locus in the B-D region of the HLA complex. We conclude that the antisera produced by this method recognize Ia-like antigens identical to or very closely associated with the HLA-D determinants.

B-Lymphocytes↗