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Biomedical subjects

D A Weigand

Publications and source records attributed to D A Weigand.

At least 19 recordsLinked to original sources
PubMed1992

Basal cell carcinoma and melanocytic nevus in the same lesion.

We report a case of basal cell carcinoma and intradermal melanocytic nevus in the same lesion. A review of the literature indicates that the simultaneous occurrence of two or more neoplasms in one specimen is a relatively infrequent finding. The pathogenesis of simultaneously occurring neoplasms remains the subject of speculation. Based on currently available evidence, we believe that the coexistence of lesions in this particular patient is most likely coincidental.

Aged↗
PubMed1989

Cutaneous immunofluorescence.

Immunofluorescence, especially direct immunofluorescence of skin, has been widely used for diagnosis of connective tissue diseases. It is principally of value for LE and may be used also as a prognostic test for SLE. It is of relatively little value for diagnosis of other connective tissue diseases and appears not to predict the outcome of undifferentiated connective tissue disease. Cutaneous immunofluorescence is widely available and has an established place in the field of dermatopathology. As with other microscopic interpretations, substantial experience, along with an understanding of basic immunopathologic principles, is necessary for reliable results. Antibodies to certain ultrastructural components of the basement membrane zone are now available. These are being evaluated for further diagnostic applications to LE and other diseases that involve the epidermal basement membrane zone.

Autoantibodies↗
PubMed1989

Late circumcision and lichen sclerosus et atrophicus of the penis.

We retrieved the records of 20 cases of lichen sclerosus et atrophicus of the penis from pathology service files at five metropolitan hospitals. Histologic sections were available for study in 16 cases. All patient records were available. All cases except one (that of a 14-year-old boy) occurred in men aged 34 to 77 years. Most of the patients (14) were circumcised for phimosis, often with meatal stenosis, and the diagnosis was made on the foreskin specimen. The remaining six patients had already been circumcised, at ages 32 to 54 years, and had lichen sclerosus et atrophicus of the glans penis. Therefore in this series of patients, the condition occurred only in patients circumcised late in life. This must be considered when a decision about neonatal circumcision is made.

Adolescent↗
PubMed1989

Direct immunofluorescence in bullous pemphigoid: effects of extent and location of lesions.

We have reevaluated the previously reported conclusion that direct immunofluorescence in bullous pemphigoid is often negative in biopsy specimens from the legs. Duplicate tests from the trunk and legs were generally of equal intensity in a prospectively evaluated series of eight patients with generalized bullous pemphigoid. Also, in 36 patients evaluated retrospectively, the intensity of the direct immunofluorescence reaction correlated roughly with extent of disease, rather than with specific anatomic region. Localized disease predictably required less vigorous treatment to achieve control, but the intensity of the immunofluorescence reaction was not similarly predictive. Direct immunofluorescence is a less useful diagnostic test in localized bullous pemphigoid than in generalized bullous pemphigoid.

Aged↗
PubMed1989

Chemical warfare.

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Chemical Warfare Agents↗
PubMed1987

Circumcision.

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Attitude↗
PubMed1987

Anesthesia in alopecia mucinosa.

A 23-year-old woman was noted to have a hypoesthetic patch over the right mandible. A biopsy specimen revealed alopecia mucinosa. Anesthesia in the involved area has been reported rarely in the literature.

Adult↗
PubMed1986

Lupus band test: anatomic regional variations in discoid lupus erythematosus.

Seventy-one patients with discoid lupus erythematosus were studied for anatomic regional variations in the lupus band test. The test was positive in 82% of biopsies from the scalp, face, neck, and upper extremity. Only three out of fourteen biopsies (21%) from the trunk were positive. Discoid lupus erythematosus could be confirmed or strongly suspected in the light microscopic sections from all cases. Truncal lesions should not be selected for the lupus band test in discoid lupus erythematosus if there are other choices.

Abdomen↗
PubMed1985

Effect of anatomic region on immunofluorescence diagnosis of bullous pemphigoid.

Forty-six patients with clinical and histologic criteria of bullous pemphigoid, and on whom direct immunofluorescence testing had been done, were analyzed by anatomic region of the lesions and by the region from which the immunofluorescence biopsy specimens were taken. One third of immunofluorescence tests from the lower extremities were negative, compared with only 3% from all other areas. The possible reasons for this result are discussed. The lower extremity should probably be avoided as a biopsy site for immunofluorescence in bullous pemphigoid.

Adult↗
PubMed1979

Leucine as an in vitro precursor to lipids in rat sciatic nerve.

The in vitro incorporation of lucine, isoleucine and pyruvate into lipids was compared and the possibility that leucine might serve as an in situ precursor to the corresponding iso fatty acids in the rat sciatic nerve was studied. The relative incorporation of 14C from leucine into lipids vs. nonlipids was 20%, and the incorporation of label into total lipids from leucine was one-halp that from pyruvate. The incorporation of label from leucine and pyruvate into sterols was nearly equivalent, but the incorporation of label into all other lipid classes from leucine was less than that from pyruvate, and the incorporation of label from isoleucine into lipids was much less in all cases. No detectable label from leucine was incorporated into brached chain fatty acids. It is concluded that leucine may be a substantial in vitro precursor to all major lipids in peripheral nerve, espeically sterols. The possibility and significance of a leucine catabolic pathway in the cytosol in relation to availability of 3-hydroxy-3-methylglutaryl CoA for sterol biosynthesis is discussed.

Animals↗
PubMed1977

Studies concerning the specificity of the effect of leucine on the turnover of proteins in muscles of control and diabetic rats.

The protein anabolic effect of branched chain amino acids was studied in isolated quarter diaphragms of rats. Protein synthesis was estimated by measuring tyrosine incorporation into muscle proteins in vitro. Tyrosine release during incubation with cycloheximide served as an index of protein degradation. In muscles from normal rats the addition of 0.5 mM leucine stimulated protein synthesis 36--38% (P less than 0.01), while equimolar isoleucine or valine, singly or in combination were ineffective. The three branched chain amino acids together stimulated no more than leucine alone. The product of leucine transamination, alpha-keto-isocaproate, did not stmino norborane-2-carboxylic acid (a leucine analogue) were ineffective. Leucine and isoleucine stimulated protein synthesis in muscles from diabetic rats.Leucine, isoleucine, valine and the norbornane amino acid but not alpha-ketoisocaproate or beta-hydroxybutyrate decreased the concentration of free tyrosine in tissues during incubation with cycloheximide; tyrosine release into the medium did not decrease significantly. Leucine caused a small decrease in total tyrosine release, (measured as the sum of free tyrosine in tissues and media), suggesting inhibition of protein degradation. The data suggest that leucine may be rate limiting for protein synthesis in muscles. The branched chain amino acids may exert a restraining effect on muscle protein catabolism during prolonged fasting and diabetes.

Animals↗
PubMed1977

Leucine and isoleucine as in vitro precursors for lipid synthesis by rat aorta.

The in vitro conversion of 14C-labeled leucine, isoleucine, and pyruvate to specific lipids was compared in rat aorta, diaphragm, anf fat pad. Total lipid specific radioactivity from all precursors was greatest in aorta. The ratio of label incorporated into polar lipids vs. neutral lipids by aorta was generally several-fold that incorporated by muscle and fat pad. The labeling of sterols in the aorta from 14C-leucine and pyruvate was equivalent. It is concluded that leucine may be a substantial precursor to polar lipids and to sterols in rat aorta.

Animals↗