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Biomedical subjects

D A Shmarov

Publications and source records attributed to D A Shmarov.

At least 19 recordsLinked to original sources
PubMed2000

[Comparative assessment of myelocariocytes cell cycle in patient with hemodepressions].

The proliferative activity of bone marrow cells was studied by flow cytofluorometry in 8 patients with aplastic anemia (AA), 6 with myelodyspastic syndrome (MDS), and 26 normal subjects aged 21-40 years. The myelokaryocyte proliferation was inhibited at the G0-1 stages of cell cycle, the number of cells in the S phase was decreased, and the coefficient of variations cV of DNA cytofluorometry values were increased in AA patients in comparison with the norm. By contrast, in MDS patients the number of cells in the G0-1 phase was decreased and that of cells in the S phase increased in comparison with the norm and with AA patients; cV was increased. These differences in the cell cycle characteristics are suggested to be used as a differential diagnostic criterion in AA and MDS.

Adult↗
PubMed2000

[The erythron system in body exposure to nitrogen oxides].

AIM: Assessment of erythron parameters in subjects exposed occupationally to nitrogen oxides polluted air versus those who work in healthy environment. MATERIALS AND METHODS: Levels of red cells, reticulocytes, packed cell volume, resistance of red cell membranes to acid buffer were measured in 95 subjects working in polluted environment and 89 healthy controls. RESULTS: Long-term (10 years and longer) contact with nitrogases entails a tendency to lowering of red cell number in the exposed subjects, significant rise of packed cell volume, increased mean cell volume of red cells, anisocytosis and reticulocytosis, high acid resistance of red cells and their membrane permeability. The causes of these changes are discussed in terms of "uneffective erythropoiesis" conception. CONCLUSION: Thus, erythron parameters can serve for indication of toxic effects of some chemicals on human body.

Adult↗
PubMed1999

[The flow cytometric analysis of hemopoietic cell proliferation. The methodological questions].

Studies of hemopoietic cell (myelokaryocyte) cycle and adequacy of applying flow DNA-cytometry for such analysis are discussed. Heterogeneity of the bone marrow, possibility of diluting a blood sample during puncture, reproducibility and stability of parameters, reliability of computer analysis of histograms demonstrating cell distribution, and correspondence of cell distribution by the cell cycle stages to the level of proliferative activity are the factors which may cause difficulties in the course of studies and while interpreting the results. Probable effects of these factors on the final results and efficacy of the method in clinical laboratory diagnosis are discussed.

Biopsy, Needle↗
PubMed1997

[Proliferative activity of lymphocytes in a culture with phytohemagglutinin and immunity parameters in acute pneumonia].

Proliferative activity of lymphocytes in a culture was assessed by flow cytometry and lymphocyte blast transformation test (LBTT) with the proliferative pool of cells (S+G2+M) from 150 patients with acute pneumonia, 30 of these with the mild form, 97 with medium-severe, and 27 with grave form; in 43 patients the disease duration was over 30 days (protracted pneumonia). In addition, levels of R.protein, homoreactant, immunoglobulins, complement components, T, B, and active T lymphocytes, phagocytosis, and granulocyte oxidative metabolism were assessed. LBTT values were in inverse relationship with the disease severity. Correlations between the studied immunity parameters were analyzed. The findings indicate that LBTT is an informative method for assessing the immune reactivity and may serve for predicting the disease course and for the differential diagnosis of pulmonary diseases.

Acute Disease↗
PubMed1997

[Flow cytometry in hematology. Methods and technique of flow cytometric analysis].

Reviews the history of flow cytometry, the philosophy of the method, main methods of cell investigation (conductometry, registration of light absorption and diffusion, cytofluorometry, study of fluorescence polarization, and analysis of a wave-shaped signal). Describes the equipment and methods for flow cytofluorometric analysis of cells, used in hematology. Characterizes the hematological analyzers with differentiated estimation of leukocytes.

Animals↗
PubMed1997

[Flow cytometry of bone marrow cells in health, anemias of different etiology and acute leukemias].

The distribution of bone marrow cells by stages of cell cycle was studied by flow cytometry in 26 normal subjects and 107 adult patients (42 with anemias and 65 with acute lymphoblastic or myeloblastic leukemias). Normal myelokaryocyte cell cycle is rather stable, which manifests by low variability of cells. In anemias and, more so, in leukemias the proliferative status of cells varies within a wide range, which is explained by unstable and ineffective hemopoiesis.

Adolescent↗
PubMed1997

[Changes in the stability of the parameters of the bone marrow cell cycle in hematologic diseases].

Cell cycle stage distribution of bone marrow cells was studied at flow cytometry in 137 hematological patients. They had acute lymphoblastic leukemia, acute myeloblastic leukemia, lymphosarcoma with leukemization, blast crisis of chronic myeloid leukemia, iron deficiency anemia, pernicious anemia, autoimmune hemolytic anemia, aplastic anemia (31, 34, 18, 12, 17, 8, 9, 8 cases, respectively). It was found that in leukemia, and to a lesser degree in anemia, stability of the cell cycle was impaired. In leukemia onset, recurrence and remission, the proportion S/(G2+M) appeared increased evidencing defective DNA synthesis by myelokaryocytes and ineffective hemopoiesis. Similar changes were seen in pernicious and aplastic anemia.

Adolescent↗
PubMed1996

[Flow cytometry in the determination of DNA aneuploidy in patients with acute leukemias].

Bone marrow cellular DNA content was measured by flow cytometry in 107 adult patients with various types of acute leukemia. DNA-aneuploidy was detected in 28 of 107 patients (26%). In 18 patients with DNA-aneuploidy bone marrow was examined throughout 2-36 months. DNA-flow cytometry of bone marrow allows to determine DNA content in leukemia cells and distinguish hypodiploid, diploid, hyperdiploid and tetraploid DNA clones in patients with acute leukemia. In some cases this method allows monitoring of the minimal residual disease.

Acute Disease↗
PubMed1993

[The mitotic cycle parameters of bone marrow cells after irradiation].

The study was performed of mitotic cycle stage distribution (G1/0, S, G2 + M) by means of flow cytometry in rats irradiated locally and totally and in 25 cancer patients receiving radiotherapy. Radiation effects manifested themselves in diminished percentage of cells in S-stage, in the block G2 + M reflecting the direct affection of myelokaryocytes. Logarithmic dependence of the quantity, of cells synthetizing DNA on radiation dose occurred in the animals in the range 0.5-6 Gy 24 hours after the exposure. Investigation of the patients' sternal puncture biopsies indicated that the proportion of S-stage cells and the value S/(G2 + M) are significant diagnostic markers of more than 1 Gy irradiation 24-48 h after the exposure. For establishing radiation overdosage these parameters proved much more informative than hemocytological indices (myelogram).

Animals↗
PubMed1989

[Flow cytofluorimetric analysis of the proliferative activity of lymphocytes in a culture with phytohemagglutinin].

Phytohemagglutinin (PHA)-effected lymphocyte blastogenesis test (LBGT) in culture has been assessed by flow cytofluorometry in 63 normal subjects and in 160 patients suffering from various pulmonary diseases. An algorithm for the estimation of cellular distribution in accordance with the mitotic cycle stages is suggested, and a computer program for the analysis of the results, based on the mitotic cycle stages, has been compiled. The lymphocyte proliferative activities have been estimated with various PHA doses. The findings have been in good correlation with the results of radionuclide studies (3H thymidine label). The described approach to LBGT assessment yields reliable results and may be used in diagnostic studies.

Adult↗
PubMed1979

[Study of the "stimulus--excitation" function in the peripheral portion of the frog taste analyzer].

The intensity of glossopharyngeal nerve afferent discharges in the frog was studied during stimulation of gustatory receptors by caffeine, acetic acid, saccharose and sodium chloride ranging widely in the concentration changes. It was shown, that the function "stimulus-exitation" for the summary discharges in response on the coffeine and acetic acid was being logaryphmic. The character of off-effects dependence on the concentration of bitter and sour stimuli. Was proved to be the same. The curve "stimulus-exitation" for the saccharose was "S" shaped, but for the sodium chloride it might be described by the power function with the exponent equal 0,8.

Acetates↗