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Biomedical subjects

D A Saville

Publications and source records attributed to D A Saville.

3 recordsLinked to original sources
PubMed1990

The effects of electroosmosis on the structure of isotachophoresis boundaries.

The influence of convection on the structure of an isotachophoretic boundary is investigated using an asymptotic method to simplify the conservation equations. To illustrate the methodology it is applied to a simple strong electrolyte system consisting of three ions and the relevant equations solved numerically. The technique, however, is of general applicability and can be useful in exploring the influence of various flow processes on analytical and preparative electrophoretic separation techniques in addition to isotachophoresis.

Diffusion↗
PubMed1989

Computer simulation and experimental validation of the electrophoretic behavior of proteins.

A mathematical model of the electrophoretic behavior of proteins is presented. The Debye-Hückel-Henry theory is used for the description of protein mobility, which has the important result of making net mobility a function of ionic strength. A net charge vs pH relationship and a diffusion coefficient are required to describe a specific protein. The model is employed for the computer simulation of three distinct electrophoretic modes: isoelectric focusing, isotachophoresis, and zone electrophoresis. The validity of the model is tested by comparing simulation with experimental data. Excellent qualitative agreement was found.

Computer Simulation↗