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Biomedical subjects

D A Kennedy

Publications and source records attributed to D A Kennedy.

35 records · Page 2Linked to original sources
PubMed1985

Microcarriers in combination with enzyme immunofiltration and immunofluroescence for the detection of herpes simplex virus antigens in culture.

A microcarrier culture system in combination with enzyme immunofiltration with a herpes simplex virus (HSV) group monoclonal antibody was found to be as sensitive as immunofluorescence for the detection of HSV type 1 (HSV-1) in cell cultures and to give specific identification at the same time as the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the appearance of the cytopathic effect with low to high inocula. Multiplicities of infection from 10(-5) to 10(1) were tested at 8 to 96 h postinfection. When applied to the identification of HSV-1 and HSV-2 in cultures of clinical samples, the system detected HSV antigens in 50% of the samples after 2 days and in 100% of the samples after 3 days. With 2 ml of microcarrier suspension and with 50 to 300 microliter per sample, several portions are available for replicate and sequential sampling without destroying the culture. The system requires only that 2 X 10(5) microcarriers be added to the culture tube at the time or before it is seeded with cells, at an extra cost of 6 cents (U.S.) per tube and little extra labor.

Antigens, Viral↗
PubMed1984

Hepatitis B follow-up among Indochinese refugees.

During 1979 and 1980 the Department of National Health and Welfare carried out a survey and later routine testing to determine the prevalence of serologic markers of hepatitis B in Indochinese refugees entering Canada. Between March and July 1981 the hepatitis-B-marker status of 220 selected refugees in the Ottawa-Hull area was reassessed. Overall, 173 (79%) of the participants had the same serologic markers when retested, but in 47 (21%) the markers had changed. The most significant changes were the loss of antigenemia in 22% of those who had been HBsAg-positive and the appearance of evidence of infection in 18% of those who had been seronegative. Most of these changes occurred among persons under 30 years of age, especially females aged 10 to 19 years.

Adolescent↗
PubMed1984

Rapid differentiation of herpes simplex virus types 1 and 2 by cytopathic effect in BS-C-1 cells.

Conditions are defined under which herpes simplex virus (HSV) type 2 produces a characteristic cytopathic effect (c.p.e.) in African green monkey kidney (BS-C-1) cells. The BS-C-1 microplate c.p.e. test was simultaneously evaluated against immunological and biological marker procedures on 359 HSV strains. A BS-C-1 cell suspension was added to serial dilutions of HSV strains in Falcon Micro Test II plates and incubated at 36.5 degrees C in a 4% CO2 atmosphere. When the infected cultures were examined microscopically, distinctive pseudopod-like extensions were seen in cultures infected with type 2 strains but not seen when the infecting virus was type 1, forming the basis for a differentiating test. The test was 100% accurate in differentiating between HSV-1 and -2 on a one test per specimen basis. The method is simple, rapid and requires no specialized reagents.

Animals↗
PubMed1981

Prevalence of hepatitis B markers in Indochinese refugees.

The prevalence of hepatitis B surface antigen (HBsAg) in 14 347 Indochinese refugees was 11.6%; the rate was significantly higher (P less than 0.01) in males (14.0%) than in females (8.8%). Most of the HBsAg-positive refugees were in the age group 20 to 29 years. Antibody to HBsAg was detected in 50.9% of the males and 46.6% of the females, a significant difference (P less than 0.01). Antibody to core antigen alone was found in 2.4% of the males and 2.2% of the females. The cumulative prevalence of one or more hepatitis B markers was significantly higher (P less than 0.001) in the males (67.3%) than in the females (57.5%). The hepatitis B e antigen and its antibody were detected in 54.8% and 39.2% respectively of 1050 HBsAg-positive blood samples. The adw, adr and ayw determinants of HBsAg were found in 23%, 35% and 42% respectively of 74 samples.

Adolescent↗
PubMed1981

Tentative protocol for the evaluation of coagulometers, based on one-stage prothrombin time.

Information on performance is invaluable to those who have to decide on the choice of an instrument. More interest is being shown in the use of coagulometers. Accordingly, a tentative protocol for the evaluation of coagulometers is presented for discussion. While it is based on the use of one-stage prothrombin time, principles of evaluation are laid down which should make it generally applicable. It is hoped that the tentative protocol will form the basis of a reference protocol and to this end comments are invited from users of coagulometers and suppliers. Comments for publication may be sent to the Editor of Clinical and Laboratory Haematology or to the Secretary of the Working Party.

Blood Coagulation Tests↗
PubMed1979

Inhibition of coronavirus 229E replication by actinomycin D.

The yields of human coronavirus 229E grown in L132 cells were markedly inhibited by actinomycin D, the 50% inhibitory dose being 0.1 micron/ml. Inhibition was maximal during the early phase of virus replication, did not appear to involve viral RNA synthesis per se, and was shown to be dependent on the input multiplicity of infection.

Cell Line↗
PubMed1977

A sophomore physical diagnosis course in an outpatient setting.

A program designed to teach sophomore medical students physical diagnosis in an outpatient setting was evaluated as an alternative to an inpatient-oriented course. A comparison of the ability of 12 students trained in an outpatient setting to examine hospitalized patients with that of students trained in the traditional inpatient-oriented program revealed no difference between the two groups. These results are consistent with the hypothesis that the clinical status of the patient and the clinical setting have little impact on students' acquisition of physical diagnosis skills. Thus, the ambulatory care setting can provide an effective alternative for inpatient-oriented physical diagnosis programs. This finding has implications for curriculum planners who are seeking ways to restructure fundamental courses that emphasize development of primary care skills.

Ambulatory Care↗
PubMed1975

A core course in family medicine for first-year medical students.

This article, the second in a series on a predoctoral curriculum in family medicine, describes in detail the processes involved in planning, implementing, and evaluating the first-year medical school core course at Hershey. The major teaching methods described are seminars, clinical correlation conferences, and practice tutorials. Student involvement in curriculum planning and teaching, flexibility and variability in modalities used, development of close relationships between faculty and students, pairing of seasoned and neophyte teachers, weekly review-preview conferences of students and faculty, and oral examinations are cited as key factors in acceptance of this curriculum by both faculty and students.

Curriculum↗
PubMed1969

The type-specific substance from Pneumococcus type 11A(43).

1. The type-specific substance from Pneumococcus type 11A(43) is a polymer containing d-glucose, d-galactose, glycerol, phosphate and O-acetyl in the approximate molecular proportions 2:2:1:1:2. 2. Removal of the O-acetyl groups with ammonia gave a compound no longer active towards type 11A antiserum. 3. Treatment of S.11A with sodium borohydride, followed by hydrolysis with alkali yielded a phosphorus-free polysaccharide, whose structure was studied by methylation and by degradation with periodate. 4. Examination of S.11A and its de-O-acetyl derivative by periodate oxidation led to the partial structure (XI) for the type-specific substance, which thus has several features in common with S.18.

Ammonia↗