Biomedical subjects

Claudio Nicolini

Publications and source records attributed to Claudio Nicolini.

39 records · Page 3Linked to original sources

PubMed2002

DNASER I: layout and data analysis.

We present the DNA analyzer (DNASER), a novel bioinstrumentation for real-time acquisition and elaboration of images from fluorescent DNA microarrays. A white light beam illuminates the target sample allowing the images grabbing on a high sensibility and wide-band charge-coupled device camera (ORCA II-Hamamatsu). This high-performance device permits to acquire images faster and of higher quality than the traditional systems. The DNA microarrays images are processed to recognize the DNA chip spots, to analyze their superficial distribution on the glass slide and to evaluate their geometric and intensity properties. Differently form conventional techniques, the spots analysis is fully automated and the DNASER does not require any additional information about the DNA microarray geometry. The DNASER hardware and software architecture is illustrated. Preliminary results are shown from experiments performed on real DNA samples.

Algorithms↗

PubMed2002

DNASER II: novel surface patterning for biomolecular microarray.

A new matrix-integral part of the new DNA microarray instrumentation DNA analyzer (DNASER) is introduced based on a novel DNA patterning on the solid support surface. Such patterning found the way to modify a glass surface for a precise positioning of small droplets of aqueous DNA solutions, without special robots (arrayers), within the boundaries of the modified regions. The physically heterogeneous surface consists of highly hydrophilic spots surrounded by a highly hydrophobic area leading to the surface patterning needed for a DNA microarray: a matrix of hydrophilic spots properly activated for immobilization of oligonucleotides has been fabricated on absolutely passive hydrophobic surface. The optimal efficiency of the above functionalitation technology of a glass-substrate in obtaining DNA microarray was confirmed by the Cy3-dCTP-labeled DNA sample, as shown by charge-coupled device images of the DNASER previously described.

Biocompatible Materials↗

PubMed2002

Hybrid organic-inorganic electrolytic capacitors.

Organic capacitors are among the most significant applications of conductive polymers, allowing the realization of lightweight and flexible dispositives. The main drawback of completely organic capacitors is the very low dielectric rigidity, due to irregular surface of the polymer-polymer interface. This paper describes our hybrid solution using aluminum electrodes, with the very important characteristic of easy connection between contacts of the discrete dispositive and electrodes. Chemical, technological, and functional tests have been conducted to estimate the effective values of this device, and natural properties of self-healing and nonpolarity have been investigated.

Aluminum↗